Study on the Preparation of Cellulose Nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application

This paper discussed on the preparation of natural CNF from kenaf bast fibre for the application as a support structure in enzyme immobilization. The treatments involved for this preparation were delignification, bleaching and high-intensity ultra-sonication process to obtain nanofibre with high cel...

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Main Authors:	Safwan Sulaiman, Mohd Noriznan Mokhtar, Mohd Nazli Naim, Azhari Samsu Baharuddin, Mohamad Amran Mohd Salleh, Alawi Sulaiman
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/9361/ http://journalarticle.ukm.my/9361/ http://journalarticle.ukm.my/9361/1/01_Safwan_Sulaiman.pdf

id	ukm-9361
recordtype	eprints
spelling	ukm-93612016-12-14T06:49:42Z http://journalarticle.ukm.my/9361/ Study on the Preparation of Cellulose Nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application Safwan Sulaiman, Mohd Noriznan Mokhtar, Mohd Nazli Naim, Azhari Samsu Baharuddin, Mohamad Amran Mohd Salleh, Alawi Sulaiman, This paper discussed on the preparation of natural CNF from kenaf bast fibre for the application as a support structure in enzyme immobilization. The treatments involved for this preparation were delignification, bleaching and high-intensity ultra-sonication process to obtain nanofibre with high cellulose content and less than 100 nm diameter. Chemical composition analysis showed the influence of each process treatment on cellulose content of raw bast fibre, bleached pulp fibre and CNF(63.67, 81.12 and 91.97%, respectively). By increasing the cellulose content and decreasing the size of cellulose fibre, it resulted in a greater number of –OH functional group on its surface that plays as important role in enzyme immobilization. FTIR spectroscopy confirms that the removal of lignin and hemicellulose from the fibre after the treatments, as well as its interaction with coupling agents and CGTase enzyme. About 62.10% of enzyme loading and 45.62% of its activity yield were obtained after immobilization. Enzymatic reaction of immobilized CGTase on CNF indicates about more than 60% relative production yield of α-CD was achieved and its reusability was able to retain about 67.0% from its initial activity after 8 cycles of reaction. Therefore, the CNF is a good potential as a support for enzyme immobilization. Universiti Kebangsaan Malaysia 2015-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9361/1/01_Safwan_Sulaiman.pdf Safwan Sulaiman, and Mohd Noriznan Mokhtar, and Mohd Nazli Naim, and Azhari Samsu Baharuddin, and Mohamad Amran Mohd Salleh, and Alawi Sulaiman, (2015) Study on the Preparation of Cellulose Nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application. Sains Malaysiana, 44 (11). pp. 1541-1550. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol44num11_2015/contentsVol44num11_2015.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This paper discussed on the preparation of natural CNF from kenaf bast fibre for the application as a support structure in enzyme immobilization. The treatments involved for this preparation were delignification, bleaching and high-intensity ultra-sonication process to obtain nanofibre with high cellulose content and less than 100 nm diameter. Chemical composition analysis showed the influence of each process treatment on cellulose content of raw bast fibre, bleached pulp fibre and CNF(63.67, 81.12 and 91.97%, respectively). By increasing the cellulose content and decreasing the size of cellulose fibre, it resulted in a greater number of –OH functional group on its surface that plays as important role in enzyme immobilization. FTIR spectroscopy confirms that the removal of lignin and hemicellulose from the fibre after the treatments, as well as its interaction with coupling agents and CGTase enzyme. About 62.10% of enzyme loading and 45.62% of its activity yield were obtained after immobilization. Enzymatic reaction of immobilized CGTase on CNF indicates about more than 60% relative production yield of α-CD was achieved and its reusability was able to retain about 67.0% from its initial activity after 8 cycles of reaction. Therefore, the CNF is a good potential as a support for enzyme immobilization.
format	Article
author	Safwan Sulaiman, Mohd Noriznan Mokhtar, Mohd Nazli Naim, Azhari Samsu Baharuddin, Mohamad Amran Mohd Salleh, Alawi Sulaiman,
spellingShingle	Safwan Sulaiman, Mohd Noriznan Mokhtar, Mohd Nazli Naim, Azhari Samsu Baharuddin, Mohamad Amran Mohd Salleh, Alawi Sulaiman, Study on the Preparation of Cellulose Nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application
author_facet	Safwan Sulaiman, Mohd Noriznan Mokhtar, Mohd Nazli Naim, Azhari Samsu Baharuddin, Mohamad Amran Mohd Salleh, Alawi Sulaiman,
author_sort	Safwan Sulaiman,
title	Study on the Preparation of Cellulose Nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application
title_short	Study on the Preparation of Cellulose Nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application
title_full	Study on the Preparation of Cellulose Nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application
title_fullStr	Study on the Preparation of Cellulose Nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application
title_full_unstemmed	Study on the Preparation of Cellulose Nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application
title_sort	study on the preparation of cellulose nanofibre (cnf) from kenaf bast fibre for enzyme immobilization application
publisher	Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/9361/ http://journalarticle.ukm.my/9361/ http://journalarticle.ukm.my/9361/1/01_Safwan_Sulaiman.pdf
first_indexed	2023-09-18T19:54:39Z
last_indexed	2023-09-18T19:54:39Z
_version_	1777406448311992320

Study on the Preparation of Cellulose Nanofibre (CNF) from kenaf bast fibre for enzyme immobilization application

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