Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa

The objectives of this study were to determine the effect of different cryoprotectants and sperm densities for long-term storage of orange mud crab, Scylla olivacea spermatozoa. Spermatozoa were obtained by homogenizing the spermatophores using a glass homogenizer in an ice-bath followed by centrifu...

Full description

Bibliographic Details
Main Authors:	Mhd Ikhwanuddin, Teng Phei Yin, Abdul Jabbar Menon, Safiah Jasmani, Ambok Bolong Abol-Munafi
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2015
Online Access:	http://journalarticle.ukm.my/9263/ http://journalarticle.ukm.my/9263/ http://journalarticle.ukm.my/9263/1/10_Mhd_Ikhwanuddin.pdf

id	ukm-9263
recordtype	eprints
spelling	ukm-92632016-12-14T06:49:23Z http://journalarticle.ukm.my/9263/ Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa Mhd Ikhwanuddin, Teng Phei Yin, Abdul Jabbar Menon, Safiah Jasmani, Ambok Bolong Abol-Munafi, The objectives of this study were to determine the effect of different cryoprotectants and sperm densities for long-term storage of orange mud crab, Scylla olivacea spermatozoa. Spermatozoa were obtained by homogenizing the spermatophores using a glass homogenizer in an ice-bath followed by centrifugation at 4°C. Spermatozoa were then suspended in calcium-free saline (Ca-F saline) containing 5% of the following cryoprotectants: Glycerol, dimethyl sulfoxide (DMSO) and methanol. Sperm which vibrated and rotated were counted as live during sperm viability assessment. Samples of spermatozoa were cooled to -196°C by two-step freezing, first to -80°C and then by plunging into liquid nitrogen (LN). Spermatozoa were gradually cooled at 1°C/min. Thawing was carried out in a 30°C water bath for 2 min. This yielded live sperm after storage in LN for 30 days. The best sperm viability was obtained from a density of 108 cells per mL in DMSO. There was no significant difference (p>0.05) among cryoprotectants toward sperm viability. However, sperm viability was significantly affected (p>0.05) by cell densities. In conclusion, DMSO gave the best protection to sperm cells of S. olivacea, but the effectiveness of DMSO as a cryoprotectant is influenced by sperm density. Universiti Kebangsaan Malaysia 2015-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9263/1/10_Mhd_Ikhwanuddin.pdf Mhd Ikhwanuddin, and Teng Phei Yin, and Abdul Jabbar Menon, and Safiah Jasmani, and Ambok Bolong Abol-Munafi, (2015) Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa. Sains Malaysiana, 44 (9). pp. 1283-1288. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil9_2015/KandunganJilid44Bil9_2015.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The objectives of this study were to determine the effect of different cryoprotectants and sperm densities for long-term storage of orange mud crab, Scylla olivacea spermatozoa. Spermatozoa were obtained by homogenizing the spermatophores using a glass homogenizer in an ice-bath followed by centrifugation at 4°C. Spermatozoa were then suspended in calcium-free saline (Ca-F saline) containing 5% of the following cryoprotectants: Glycerol, dimethyl sulfoxide (DMSO) and methanol. Sperm which vibrated and rotated were counted as live during sperm viability assessment. Samples of spermatozoa were cooled to -196°C by two-step freezing, first to -80°C and then by plunging into liquid nitrogen (LN). Spermatozoa were gradually cooled at 1°C/min. Thawing was carried out in a 30°C water bath for 2 min. This yielded live sperm after storage in LN for 30 days. The best sperm viability was obtained from a density of 108 cells per mL in DMSO. There was no significant difference (p>0.05) among cryoprotectants toward sperm viability. However, sperm viability was significantly affected (p>0.05) by cell densities. In conclusion, DMSO gave the best protection to sperm cells of S. olivacea, but the effectiveness of DMSO as a cryoprotectant is influenced by sperm density.
format	Article
author	Mhd Ikhwanuddin, Teng Phei Yin, Abdul Jabbar Menon, Safiah Jasmani, Ambok Bolong Abol-Munafi,
spellingShingle	Mhd Ikhwanuddin, Teng Phei Yin, Abdul Jabbar Menon, Safiah Jasmani, Ambok Bolong Abol-Munafi, Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
author_facet	Mhd Ikhwanuddin, Teng Phei Yin, Abdul Jabbar Menon, Safiah Jasmani, Ambok Bolong Abol-Munafi,
author_sort	Mhd Ikhwanuddin,
title	Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
title_short	Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
title_full	Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
title_fullStr	Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
title_full_unstemmed	Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
title_sort	effect of different cryoprotectants and sperm densities of orange mud crab, scylla olivacea (herbst, 1796) for long-term storage of spermatozoa
publisher	Universiti Kebangsaan Malaysia
publishDate	2015
url	http://journalarticle.ukm.my/9263/ http://journalarticle.ukm.my/9263/ http://journalarticle.ukm.my/9263/1/10_Mhd_Ikhwanuddin.pdf
first_indexed	2023-09-18T19:54:23Z
last_indexed	2023-09-18T19:54:23Z
_version_	1777406431993004032

Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa

Similar Items