Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features
Colorectal cancer develops in a multi-step process and is associated with genetic alterations. Blocking apoptosis is a key factor to unlimited cell proliferation and immortalization. Survivin expression inhibits the programmed cell death process, apoptosis. This molecule could play a potential rol...
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Penerbit Universiti Kebangsaan Malaysia
2014
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| Online Access: | http://journalarticle.ukm.my/8677/ http://journalarticle.ukm.my/8677/ http://journalarticle.ukm.my/8677/1/43_2_05.pdf |
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ukm-86772016-12-14T06:47:51Z http://journalarticle.ukm.my/8677/ Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features Said Saleh, King, Alfred Yin Lam Yik, Hong Ho Colorectal cancer develops in a multi-step process and is associated with genetic alterations. Blocking apoptosis is a key factor to unlimited cell proliferation and immortalization. Survivin expression inhibits the programmed cell death process, apoptosis. This molecule could play a potential role in cancer development. Survivin is an attractive target for clinical trials to develop cancer treatment. RNA was prepared from colorectal tumor and adjacent non-tumor tissues and then transcribed to complementary DNA. Human survivin mRNA levels were quantitatively measured by real time polymerase chain reaction (PCR) in tumor and adjacent non-tumor tissues. Expression levels of survivin mRNA in adenocarcinomas were significantly higher than in non-tumor mucosa (p < 0.0001). The expressions of survivin mRNA in adenocarcinomas were related to the degree of differentiation (survivin; p=0.034). No difference was found with other clinicopathological features. These findings indicate survivin role in colorectal carcinogenesis. Survivin could be used as a potential diagnostic and prognostic marker in colorectal cancer. Successful inhibition of this molecule could lead to the development of a new drug for cancer therapy. Penerbit Universiti Kebangsaan Malaysia 2014-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8677/1/43_2_05.pdf Said Saleh, and King, Alfred Yin Lam and Yik, Hong Ho (2014) Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features. Malaysian Applied Biology, 43 (2). pp. 35-40. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=460&catid=59:current-view&Itemid=56 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Kebangasaan Malaysia |
| building |
UKM Institutional Repository |
| collection |
Online Access |
| language |
English |
| description |
Colorectal cancer develops in a multi-step process and is associated with genetic alterations. Blocking apoptosis is a key
factor to unlimited cell proliferation and immortalization. Survivin expression inhibits the programmed cell death process,
apoptosis. This molecule could play a potential role in cancer development. Survivin is an attractive target for clinical trials to
develop cancer treatment. RNA was prepared from colorectal tumor and adjacent non-tumor tissues and then transcribed to
complementary DNA. Human survivin mRNA levels were quantitatively measured by real time polymerase chain reaction
(PCR) in tumor and adjacent non-tumor tissues. Expression levels of survivin mRNA in adenocarcinomas were significantly
higher than in non-tumor mucosa (p < 0.0001). The expressions of survivin mRNA in adenocarcinomas were related to the
degree of differentiation (survivin; p=0.034). No difference was found with other clinicopathological features. These findings
indicate survivin role in colorectal carcinogenesis. Survivin could be used as a potential diagnostic and prognostic marker in
colorectal cancer. Successful inhibition of this molecule could lead to the development of a new drug for cancer therapy. |
| format |
Article |
| author |
Said Saleh, King, Alfred Yin Lam Yik, Hong Ho |
| spellingShingle |
Said Saleh, King, Alfred Yin Lam Yik, Hong Ho Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features |
| author_facet |
Said Saleh, King, Alfred Yin Lam Yik, Hong Ho |
| author_sort |
Said Saleh, |
| title |
Survivin expression in colorectal: real time
polymerase chain reaction and correlation with
clinicopathological features |
| title_short |
Survivin expression in colorectal: real time
polymerase chain reaction and correlation with
clinicopathological features |
| title_full |
Survivin expression in colorectal: real time
polymerase chain reaction and correlation with
clinicopathological features |
| title_fullStr |
Survivin expression in colorectal: real time
polymerase chain reaction and correlation with
clinicopathological features |
| title_full_unstemmed |
Survivin expression in colorectal: real time
polymerase chain reaction and correlation with
clinicopathological features |
| title_sort |
survivin expression in colorectal: real time
polymerase chain reaction and correlation with
clinicopathological features |
| publisher |
Penerbit Universiti Kebangsaan Malaysia |
| publishDate |
2014 |
| url |
http://journalarticle.ukm.my/8677/ http://journalarticle.ukm.my/8677/ http://journalarticle.ukm.my/8677/1/43_2_05.pdf |
| first_indexed |
2023-09-18T19:52:56Z |
| last_indexed |
2023-09-18T19:52:56Z |
| _version_ |
1777406340352704512 |