Survivin expression in colorectal: real time polymerase chain reaction and correlation with clinicopathological features
Colorectal cancer develops in a multi-step process and is associated with genetic alterations. Blocking apoptosis is a key factor to unlimited cell proliferation and immortalization. Survivin expression inhibits the programmed cell death process, apoptosis. This molecule could play a potential rol...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2014
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| Online Access: | http://journalarticle.ukm.my/8677/ http://journalarticle.ukm.my/8677/ http://journalarticle.ukm.my/8677/1/43_2_05.pdf |
| Summary: | Colorectal cancer develops in a multi-step process and is associated with genetic alterations. Blocking apoptosis is a key
factor to unlimited cell proliferation and immortalization. Survivin expression inhibits the programmed cell death process,
apoptosis. This molecule could play a potential role in cancer development. Survivin is an attractive target for clinical trials to
develop cancer treatment. RNA was prepared from colorectal tumor and adjacent non-tumor tissues and then transcribed to
complementary DNA. Human survivin mRNA levels were quantitatively measured by real time polymerase chain reaction
(PCR) in tumor and adjacent non-tumor tissues. Expression levels of survivin mRNA in adenocarcinomas were significantly
higher than in non-tumor mucosa (p < 0.0001). The expressions of survivin mRNA in adenocarcinomas were related to the
degree of differentiation (survivin; p=0.034). No difference was found with other clinicopathological features. These findings
indicate survivin role in colorectal carcinogenesis. Survivin could be used as a potential diagnostic and prognostic marker in
colorectal cancer. Successful inhibition of this molecule could lead to the development of a new drug for cancer therapy. |
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