Asymbiotic seed germination and seedling development of Vanda dearei

The effects of basal media, complex additives, plant growth regulators and carbon sources on in vitro seed germination and seedling development of Vanda dearei are reported. Immature seeds from four months old capsule were used as plant materials. All cultures were grown under 24h light at 25±2ºC....

Full description

Bibliographic Details
Main Authors:	Roslina, J., Devina, D., Hartinie, M., Sharon, J.S., Jualang, A.G.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/8676/ http://journalarticle.ukm.my/8676/ http://journalarticle.ukm.my/8676/1/43_2_04.pdf

id	ukm-8676
recordtype	eprints
spelling	ukm-86762016-12-14T06:47:51Z http://journalarticle.ukm.my/8676/ Asymbiotic seed germination and seedling development of Vanda dearei Roslina, J. Devina, D. Hartinie, M. Sharon, J.S. Jualang, A.G. The effects of basal media, complex additives, plant growth regulators and carbon sources on in vitro seed germination and seedling development of Vanda dearei are reported. Immature seeds from four months old capsule were used as plant materials. All cultures were grown under 24h light at 25±2ºC. Results showed that seeds cultured on Knudson C (KC) basal medium germinated after 25 days with 63.0±3.2% germination rate followed by half-strength Murashige & Skoog (½MS) (45.4±10.4%) and Vacin and Went (VW) (41.8±4.0%). Addition of 0.5% (w/v) yeast extract significantly enhanced (85.9±0.7%) seed germination and shortened germination time to 23 days. A NAA at 0.1mg/l had similar performance (80.2±20.5%), however, this treatment delayed seed germination and induced necrosis to protocorm development. Sucrose at 1% (w/v) also enhanced seed germination (98.3±2.3%), while glucose and fructose treatments showed moderate effects. For growth and development of protocorms, KC basal media recorded the highest percentage of protocorm with root (37.0±4.3%), mean number of leaf (4.50±1.00) and mean number of roots produced (2.0±0.6) with largest leaf area (3.7x2.3mm) and longest root length (11.7±8.4mm). Addition of 20% (v/v) coconut water significantly improved protocorm development and shoot growth. Penerbit Universiti Kebangsaan Malaysia 2014-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8676/1/43_2_04.pdf Roslina, J. and Devina, D. and Hartinie, M. and Sharon, J.S. and Jualang, A.G. (2014) Asymbiotic seed germination and seedling development of Vanda dearei. Malaysian Applied Biology, 43 (2). pp. 25-33. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=460&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The effects of basal media, complex additives, plant growth regulators and carbon sources on in vitro seed germination and seedling development of Vanda dearei are reported. Immature seeds from four months old capsule were used as plant materials. All cultures were grown under 24h light at 25±2ºC. Results showed that seeds cultured on Knudson C (KC) basal medium germinated after 25 days with 63.0±3.2% germination rate followed by half-strength Murashige & Skoog (½MS) (45.4±10.4%) and Vacin and Went (VW) (41.8±4.0%). Addition of 0.5% (w/v) yeast extract significantly enhanced (85.9±0.7%) seed germination and shortened germination time to 23 days. A NAA at 0.1mg/l had similar performance (80.2±20.5%), however, this treatment delayed seed germination and induced necrosis to protocorm development. Sucrose at 1% (w/v) also enhanced seed germination (98.3±2.3%), while glucose and fructose treatments showed moderate effects. For growth and development of protocorms, KC basal media recorded the highest percentage of protocorm with root (37.0±4.3%), mean number of leaf (4.50±1.00) and mean number of roots produced (2.0±0.6) with largest leaf area (3.7x2.3mm) and longest root length (11.7±8.4mm). Addition of 20% (v/v) coconut water significantly improved protocorm development and shoot growth.
format	Article
author	Roslina, J. Devina, D. Hartinie, M. Sharon, J.S. Jualang, A.G.
spellingShingle	Roslina, J. Devina, D. Hartinie, M. Sharon, J.S. Jualang, A.G. Asymbiotic seed germination and seedling development of Vanda dearei
author_facet	Roslina, J. Devina, D. Hartinie, M. Sharon, J.S. Jualang, A.G.
author_sort	Roslina, J.
title	Asymbiotic seed germination and seedling development of Vanda dearei
title_short	Asymbiotic seed germination and seedling development of Vanda dearei
title_full	Asymbiotic seed germination and seedling development of Vanda dearei
title_fullStr	Asymbiotic seed germination and seedling development of Vanda dearei
title_full_unstemmed	Asymbiotic seed germination and seedling development of Vanda dearei
title_sort	asymbiotic seed germination and seedling development of vanda dearei
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/8676/ http://journalarticle.ukm.my/8676/ http://journalarticle.ukm.my/8676/1/43_2_04.pdf
first_indexed	2023-09-18T19:52:56Z
last_indexed	2023-09-18T19:52:56Z
_version_	1777406340210098176

Asymbiotic seed germination and seedling development of Vanda dearei

Similar Items