Development of the Double Digest Selective Label (DDSL) Typing Technique and Its Application to Staphylococcus aureus Epidemiology

Bacterial typing is a key technology in human and veterinary medicine, community health, consumer protection and in agricultural research. The importance of the development of epidemiological tracking tools is underlined by numerous outbreaks of diseases due to bacterial pathogens. Particularly impo...

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Main Authors:	V. Terletskiy, V. Tyshchenko, D.A. Myrzakozha, O.O. Zhanserkenova, Y.S. Ussenbekov, Nurbek L. Adaev
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/8159/ http://journalarticle.ukm.my/8159/ http://journalarticle.ukm.my/8159/1/20_V._Terletskiy.pdf

id	ukm-8159
recordtype	eprints
spelling	ukm-81592016-12-14T06:46:24Z http://journalarticle.ukm.my/8159/ Development of the Double Digest Selective Label (DDSL) Typing Technique and Its Application to Staphylococcus aureus Epidemiology V. Terletskiy, V. Tyshchenko, D.A. Myrzakozha, O.O. Zhanserkenova, Y.S. Ussenbekov, Nurbek L. Adaev, Bacterial typing is a key technology in human and veterinary medicine, community health, consumer protection and in agricultural research. The importance of the development of epidemiological tracking tools is underlined by numerous outbreaks of diseases due to bacterial pathogens. Particularly important is tracing pathogen dissemination in ‘real time’ i.e. to use a fast typing technique to distinguish between clonally related (epidemic) strains and unrelated (sporadic) strains. The aim of the research was to develop a fast discriminatory molecular typing technique - double digest selective label (DDSL) for Staphylococcus aureus isolates and to compare typing data with that obtained by pulsed-field gel electrophoresis. In this new typing method, large DNA fragments are produced with a restriction enzyme commonly used for PFGE but are trimmed by a second enzyme to a size which can be separated on a conventional agarose gel within a short period of time. Selective labelling of a subset of the numerous restriction fragments gives a distinct banding pattern for each isolate. Discriminatory power obtained with DDSL calculated over two different sets was higher than that of pulsed-field gel electrophoresis. Clusters of identical isolates were further resolved to unique DDSL strains. Two combinations of restriction enzymes for DDSL technique has been proposed with approximately equal discriminatory power. It has been demonstrated that DDSL approach is a fast, discriminatory alternative to other typing techniques suitable for short-term epidemiological studies. Universiti Kebangsaan Malaysia 2014-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8159/1/20_V._Terletskiy.pdf V. Terletskiy, and V. Tyshchenko, and D.A. Myrzakozha, and O.O. Zhanserkenova, and Y.S. Ussenbekov, and Nurbek L. Adaev, (2014) Development of the Double Digest Selective Label (DDSL) Typing Technique and Its Application to Staphylococcus aureus Epidemiology. Sains Malaysiana, 43 (12). pp. 1965-1972. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Bacterial typing is a key technology in human and veterinary medicine, community health, consumer protection and in agricultural research. The importance of the development of epidemiological tracking tools is underlined by numerous outbreaks of diseases due to bacterial pathogens. Particularly important is tracing pathogen dissemination in ‘real time’ i.e. to use a fast typing technique to distinguish between clonally related (epidemic) strains and unrelated (sporadic) strains. The aim of the research was to develop a fast discriminatory molecular typing technique - double digest selective label (DDSL) for Staphylococcus aureus isolates and to compare typing data with that obtained by pulsed-field gel electrophoresis. In this new typing method, large DNA fragments are produced with a restriction enzyme commonly used for PFGE but are trimmed by a second enzyme to a size which can be separated on a conventional agarose gel within a short period of time. Selective labelling of a subset of the numerous restriction fragments gives a distinct banding pattern for each isolate. Discriminatory power obtained with DDSL calculated over two different sets was higher than that of pulsed-field gel electrophoresis. Clusters of identical isolates were further resolved to unique DDSL strains. Two combinations of restriction enzymes for DDSL technique has been proposed with approximately equal discriminatory power. It has been demonstrated that DDSL approach is a fast, discriminatory alternative to other typing techniques suitable for short-term epidemiological studies.
format	Article
author	V. Terletskiy, V. Tyshchenko, D.A. Myrzakozha, O.O. Zhanserkenova, Y.S. Ussenbekov, Nurbek L. Adaev,
spellingShingle	V. Terletskiy, V. Tyshchenko, D.A. Myrzakozha, O.O. Zhanserkenova, Y.S. Ussenbekov, Nurbek L. Adaev, Development of the Double Digest Selective Label (DDSL) Typing Technique and Its Application to Staphylococcus aureus Epidemiology
author_facet	V. Terletskiy, V. Tyshchenko, D.A. Myrzakozha, O.O. Zhanserkenova, Y.S. Ussenbekov, Nurbek L. Adaev,
author_sort	V. Terletskiy,
title	Development of the Double Digest Selective Label (DDSL) Typing Technique and Its Application to Staphylococcus aureus Epidemiology
title_short	Development of the Double Digest Selective Label (DDSL) Typing Technique and Its Application to Staphylococcus aureus Epidemiology
title_full	Development of the Double Digest Selective Label (DDSL) Typing Technique and Its Application to Staphylococcus aureus Epidemiology
title_fullStr	Development of the Double Digest Selective Label (DDSL) Typing Technique and Its Application to Staphylococcus aureus Epidemiology
title_full_unstemmed	Development of the Double Digest Selective Label (DDSL) Typing Technique and Its Application to Staphylococcus aureus Epidemiology
title_sort	development of the double digest selective label (ddsl) typing technique and its application to staphylococcus aureus epidemiology
publisher	Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/8159/ http://journalarticle.ukm.my/8159/ http://journalarticle.ukm.my/8159/1/20_V._Terletskiy.pdf
first_indexed	2023-09-18T19:51:39Z
last_indexed	2023-09-18T19:51:39Z
_version_	1777406259033538560

Development of the Double Digest Selective Label (DDSL) Typing Technique and Its Application to Staphylococcus aureus Epidemiology

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