Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia

Enterobacter sakazakii previously known as ‘yellow-pigmented E. cloacae’ has been classified as a new genus ‘Cronobacter’ based on taxonomic analysis and geno-and phenotypic evaluation. This pathogenic organism has been associated with rare form of infant meningitis and necrotizing enterocolitis (NE...

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Main Authors:	Norrakiah Abdullah Sani, Masomeh Ghassem, Abdul Salam Babji, Uma Priya Kupusamy, Norizan Jaafar
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/8145/ http://journalarticle.ukm.my/8145/ http://journalarticle.ukm.my/8145/1/06_Norrakiah_Abdullah.pdf

id	ukm-8145
recordtype	eprints
spelling	ukm-81452016-12-14T06:46:21Z http://journalarticle.ukm.my/8145/ Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia Norrakiah Abdullah Sani, Masomeh Ghassem, Abdul Salam Babji, Uma Priya Kupusamy, Norizan Jaafar, Enterobacter sakazakii previously known as ‘yellow-pigmented E. cloacae’ has been classified as a new genus ‘Cronobacter’ based on taxonomic analysis and geno-and phenotypic evaluation. This pathogenic organism has been associated with rare form of infant meningitis and necrotizing enterocolitis (NEC) with high mortality rate (40-80%). Some cases have been linked to the consumption of contaminated powdered infant formula milk (PIF). The objective of this study was to determine the presence of Cronobacter spp. in PIF sold in Malaysia. A selective chromogenic agar, Brilliance Enterobacter sakazakii (DFI, Oxoid), was used for detection of Cronobacter strains. Presumptive Cronobacter isolates were identified using biochemical tests (API 20E and MicrogenTM) and molecular assays (SYBR Green Real-time PCR and 16S ribosomal DNA sequencing). All presumptive Cronobacter strains produced typical blue-green colonies and non-Cronobacter strains produced yellow colonies on Brilliance Enterobacter sakazakii agar (DFI formulation). A total of 12 presumptive isolates were selected from DFI agar and identified with biochemical and molecular tests. The results indicated prevalence of 12.5% C. sakazakii contamination from 72 PIF samples. Molecular detection methods such as Real-time PCR and 16S rDNA proved to have higher identification percentage compared to the biochemical tests. In this study, it was observed that molecular assays were suitable means for sensitive identification of Cronobacter strains in PIF samples. Universiti Kebangsaan Malaysia 2014-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8145/1/06_Norrakiah_Abdullah.pdf Norrakiah Abdullah Sani, and Masomeh Ghassem, and Abdul Salam Babji, and Uma Priya Kupusamy, and Norizan Jaafar, (2014) Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia. Sains Malaysiana, 43 (12). pp. 1855-1863. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Enterobacter sakazakii previously known as ‘yellow-pigmented E. cloacae’ has been classified as a new genus ‘Cronobacter’ based on taxonomic analysis and geno-and phenotypic evaluation. This pathogenic organism has been associated with rare form of infant meningitis and necrotizing enterocolitis (NEC) with high mortality rate (40-80%). Some cases have been linked to the consumption of contaminated powdered infant formula milk (PIF). The objective of this study was to determine the presence of Cronobacter spp. in PIF sold in Malaysia. A selective chromogenic agar, Brilliance Enterobacter sakazakii (DFI, Oxoid), was used for detection of Cronobacter strains. Presumptive Cronobacter isolates were identified using biochemical tests (API 20E and MicrogenTM) and molecular assays (SYBR Green Real-time PCR and 16S ribosomal DNA sequencing). All presumptive Cronobacter strains produced typical blue-green colonies and non-Cronobacter strains produced yellow colonies on Brilliance Enterobacter sakazakii agar (DFI formulation). A total of 12 presumptive isolates were selected from DFI agar and identified with biochemical and molecular tests. The results indicated prevalence of 12.5% C. sakazakii contamination from 72 PIF samples. Molecular detection methods such as Real-time PCR and 16S rDNA proved to have higher identification percentage compared to the biochemical tests. In this study, it was observed that molecular assays were suitable means for sensitive identification of Cronobacter strains in PIF samples.
format	Article
author	Norrakiah Abdullah Sani, Masomeh Ghassem, Abdul Salam Babji, Uma Priya Kupusamy, Norizan Jaafar,
spellingShingle	Norrakiah Abdullah Sani, Masomeh Ghassem, Abdul Salam Babji, Uma Priya Kupusamy, Norizan Jaafar, Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia
author_facet	Norrakiah Abdullah Sani, Masomeh Ghassem, Abdul Salam Babji, Uma Priya Kupusamy, Norizan Jaafar,
author_sort	Norrakiah Abdullah Sani,
title	Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia
title_short	Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia
title_full	Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia
title_fullStr	Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia
title_full_unstemmed	Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia
title_sort	incidence of cronobacter sakazakii in powdered infant formula milk available in malaysia
publisher	Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/8145/ http://journalarticle.ukm.my/8145/ http://journalarticle.ukm.my/8145/1/06_Norrakiah_Abdullah.pdf
first_indexed	2023-09-18T19:51:37Z
last_indexed	2023-09-18T19:51:37Z
_version_	1777406256987766784

Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia

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