Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species

Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species

Xanthine oxidase (XO) is an enzyme that catalyzes the metabolism of hypoxanthine and xanthine into uric acid. XO also serves as an important biological source of free radicals that contribute to oxidative damage involved in many pathological processes. Antioxidant effects of several Primulaceae spec...

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Main Authors: Norlida Mamat, Jamia Azdina Jamal, Ibrahim Jantan, Khairana Husain
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2014
Online Access:http://journalarticle.ukm.my/8142/
http://journalarticle.ukm.my/8142/
http://journalarticle.ukm.my/8142/1/03_Norlida_Mamat.pdf
id ukm-8142
recordtype eprints
spelling ukm-81422016-12-14T06:46:20Z http://journalarticle.ukm.my/8142/ Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species Norlida Mamat, Jamia Azdina Jamal, Ibrahim Jantan, Khairana Husain, Xanthine oxidase (XO) is an enzyme that catalyzes the metabolism of hypoxanthine and xanthine into uric acid. XO also serves as an important biological source of free radicals that contribute to oxidative damage involved in many pathological processes. Antioxidant effects of several Primulaceae species have been reported but their XO inhibitory activity has not been investigated. Thus, this study was conducted to determine the XO inhibitory and free radical scavenging activities of Primulaceae species and to correlate these activities with their total phenolic contents (TPC). A total of 129 extracts of different plant parts of twelve Primulaceae species were assayed for XO inhibition spectrophotometrically at 290 nm using allopurinol as a positive control. The antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and TPC of the extracts were determined by the Folin-Ciocalteau method. The Pearson correlation analysis indicated that the TPC of the extracts showed moderate positive correlations with XO inhibition (r=0.31, p<0.05) and DPPH antioxidant activity (r=0.31, p<0.05) for all of the dichloromethane extracts. Amongst the extracts tested, the dichloromethane extract of the roots of Labisia pumila var. alata showed the strongest inhibitory effects for XO (IC50 4.8 μg/mL) and DPPH free radical capacity (IC50 1.7 μg/mL). The results suggested that Primulaceae species, particularly the dichloromethane extract of L. pumila var. alata roots, are the potential source of useful leads for the development of XO inhibitors. Universiti Kebangsaan Malaysia 2014-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8142/1/03_Norlida_Mamat.pdf Norlida Mamat, and Jamia Azdina Jamal, and Ibrahim Jantan, and Khairana Husain, (2014) Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species. Sains Malaysiana, 43 (12). pp. 1827-1833. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description Xanthine oxidase (XO) is an enzyme that catalyzes the metabolism of hypoxanthine and xanthine into uric acid. XO also serves as an important biological source of free radicals that contribute to oxidative damage involved in many pathological processes. Antioxidant effects of several Primulaceae species have been reported but their XO inhibitory activity has not been investigated. Thus, this study was conducted to determine the XO inhibitory and free radical scavenging activities of Primulaceae species and to correlate these activities with their total phenolic contents (TPC). A total of 129 extracts of different plant parts of twelve Primulaceae species were assayed for XO inhibition spectrophotometrically at 290 nm using allopurinol as a positive control. The antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and TPC of the extracts were determined by the Folin-Ciocalteau method. The Pearson correlation analysis indicated that the TPC of the extracts showed moderate positive correlations with XO inhibition (r=0.31, p<0.05) and DPPH antioxidant activity (r=0.31, p<0.05) for all of the dichloromethane extracts. Amongst the extracts tested, the dichloromethane extract of the roots of Labisia pumila var. alata showed the strongest inhibitory effects for XO (IC50 4.8 μg/mL) and DPPH free radical capacity (IC50 1.7 μg/mL). The results suggested that Primulaceae species, particularly the dichloromethane extract of L. pumila var. alata roots, are the potential source of useful leads for the development of XO inhibitors.
format Article
author Norlida Mamat,
Jamia Azdina Jamal,
Ibrahim Jantan,
Khairana Husain,
spellingShingle Norlida Mamat,
Jamia Azdina Jamal,
Ibrahim Jantan,
Khairana Husain,
Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species
author_facet Norlida Mamat,
Jamia Azdina Jamal,
Ibrahim Jantan,
Khairana Husain,
author_sort Norlida Mamat,
title Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species
title_short Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species
title_full Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species
title_fullStr Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species
title_full_unstemmed Xanthine oxidase inhibitory and DPPH radical scavenging activities of some primulaceae species
title_sort xanthine oxidase inhibitory and dpph radical scavenging activities of some primulaceae species
publisher Universiti Kebangsaan Malaysia
publishDate 2014
url http://journalarticle.ukm.my/8142/
http://journalarticle.ukm.my/8142/
http://journalarticle.ukm.my/8142/1/03_Norlida_Mamat.pdf
first_indexed 2023-09-18T19:51:36Z
last_indexed 2023-09-18T19:51:36Z
_version_ 1777406256527441920

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