Antioxidant Activity of Rhodomyrtus tomentosa (Kemunting) Fruits and Its Effect on Lipid Profile in Induced-cholesterol New Zealand White Rabbits

The objective of this study was to determine the antioxidant activity of Rhodomyrtus tomentosa fruit extract and its effect on triacylglycerides (TG), total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL) and lipid peroxidation in induced-cholesterol New Zealand White...

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Main Authors:	Muhamad Fahrin Maskam, Jamaludin Mohamad, Mahmood Ameen Abdulla, Adlin Afzan, Isa Wasiman
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/8037/ http://journalarticle.ukm.my/8037/ http://journalarticle.ukm.my/8037/1/05_Muhamad_Fahrin.pdf

id	ukm-8037
recordtype	eprints
spelling	ukm-80372016-12-14T06:46:01Z http://journalarticle.ukm.my/8037/ Antioxidant Activity of Rhodomyrtus tomentosa (Kemunting) Fruits and Its Effect on Lipid Profile in Induced-cholesterol New Zealand White Rabbits Muhamad Fahrin Maskam, Jamaludin Mohamad, Mahmood Ameen Abdulla, Adlin Afzan, Isa Wasiman, The objective of this study was to determine the antioxidant activity of Rhodomyrtus tomentosa fruit extract and its effect on triacylglycerides (TG), total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL) and lipid peroxidation in induced-cholesterol New Zealand White Rabbits. In DPPH assay, at concentration of 200 ug/mL methanol extract give 62.13% inhibition of DPPH free radicals with IC50 of 107 μg/mL. Similarly, in FRAP assay the methanol extract at concentration of 500 ug/mL showed the highest absorbance (0.16) for antioxidant activity. Whereas, in metal chelating assay the methanol extract at concentration of 100 mg/mL exhibited 36% inhibition of metal chelating ions. The antioxidant activities were due to the presence of phenolics compounds of quinic acid, gallic acid and caffeic acid, which were identified with Q-TQF MS. Total phenolic and total flavonoid content was the highest in water extract at 66.515 mg of GAE/g and 1.828 mg of QE/g, respectively. The water extract of R. tomentosa was non-toxic at LC50 = 616.083 μg/mL. The white New Zealand rabbits group A was fed by oral gavages with normal diet; Group B cholesterol 1% diet; group C cholesterol 1% diet with fruit extract 50 mg/kg and Group D cholesterol 1% diet with simvastatin standard drug 5 mg/kg. The rabbits in group C has a significantly reduced (p<0.05) total cholesterol (TC), low density lipoprotein (LDL) and significantly increased (p<0.05) high density lipoprotein (HDL) and triacylglycerides (TG) as compared with group B. The lipid peroxidation was reduced significantly (p<0.05) as indicated by the low TBARs-MDA level in group C. Thus, these results showed that the R. tomentosa fruit extracts was able to reduce cholesterol level and increased HDL level which can prevent the formation of atherosclerosis in New Zealand white rabbits. Universiti Kebangsaan Malaysia 2014-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8037/1/05_Muhamad_Fahrin.pdf Muhamad Fahrin Maskam, and Jamaludin Mohamad, and Mahmood Ameen Abdulla, and Adlin Afzan, and Isa Wasiman, (2014) Antioxidant Activity of Rhodomyrtus tomentosa (Kemunting) Fruits and Its Effect on Lipid Profile in Induced-cholesterol New Zealand White Rabbits. Sains Malaysiana, 43 (11). pp. 1673-1684. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The objective of this study was to determine the antioxidant activity of Rhodomyrtus tomentosa fruit extract and its effect on triacylglycerides (TG), total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL) and lipid peroxidation in induced-cholesterol New Zealand White Rabbits. In DPPH assay, at concentration of 200 ug/mL methanol extract give 62.13% inhibition of DPPH free radicals with IC50 of 107 μg/mL. Similarly, in FRAP assay the methanol extract at concentration of 500 ug/mL showed the highest absorbance (0.16) for antioxidant activity. Whereas, in metal chelating assay the methanol extract at concentration of 100 mg/mL exhibited 36% inhibition of metal chelating ions. The antioxidant activities were due to the presence of phenolics compounds of quinic acid, gallic acid and caffeic acid, which were identified with Q-TQF MS. Total phenolic and total flavonoid content was the highest in water extract at 66.515 mg of GAE/g and 1.828 mg of QE/g, respectively. The water extract of R. tomentosa was non-toxic at LC50 = 616.083 μg/mL. The white New Zealand rabbits group A was fed by oral gavages with normal diet; Group B cholesterol 1% diet; group C cholesterol 1% diet with fruit extract 50 mg/kg and Group D cholesterol 1% diet with simvastatin standard drug 5 mg/kg. The rabbits in group C has a significantly reduced (p<0.05) total cholesterol (TC), low density lipoprotein (LDL) and significantly increased (p<0.05) high density lipoprotein (HDL) and triacylglycerides (TG) as compared with group B. The lipid peroxidation was reduced significantly (p<0.05) as indicated by the low TBARs-MDA level in group C. Thus, these results showed that the R. tomentosa fruit extracts was able to reduce cholesterol level and increased HDL level which can prevent the formation of atherosclerosis in New Zealand white rabbits.
format	Article
author	Muhamad Fahrin Maskam, Jamaludin Mohamad, Mahmood Ameen Abdulla, Adlin Afzan, Isa Wasiman,
spellingShingle	Muhamad Fahrin Maskam, Jamaludin Mohamad, Mahmood Ameen Abdulla, Adlin Afzan, Isa Wasiman, Antioxidant Activity of Rhodomyrtus tomentosa (Kemunting) Fruits and Its Effect on Lipid Profile in Induced-cholesterol New Zealand White Rabbits
author_facet	Muhamad Fahrin Maskam, Jamaludin Mohamad, Mahmood Ameen Abdulla, Adlin Afzan, Isa Wasiman,
author_sort	Muhamad Fahrin Maskam,
title	Antioxidant Activity of Rhodomyrtus tomentosa (Kemunting) Fruits and Its Effect on Lipid Profile in Induced-cholesterol New Zealand White Rabbits
title_short	Antioxidant Activity of Rhodomyrtus tomentosa (Kemunting) Fruits and Its Effect on Lipid Profile in Induced-cholesterol New Zealand White Rabbits
title_full	Antioxidant Activity of Rhodomyrtus tomentosa (Kemunting) Fruits and Its Effect on Lipid Profile in Induced-cholesterol New Zealand White Rabbits
title_fullStr	Antioxidant Activity of Rhodomyrtus tomentosa (Kemunting) Fruits and Its Effect on Lipid Profile in Induced-cholesterol New Zealand White Rabbits
title_full_unstemmed	Antioxidant Activity of Rhodomyrtus tomentosa (Kemunting) Fruits and Its Effect on Lipid Profile in Induced-cholesterol New Zealand White Rabbits
title_sort	antioxidant activity of rhodomyrtus tomentosa (kemunting) fruits and its effect on lipid profile in induced-cholesterol new zealand white rabbits
publisher	Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/8037/ http://journalarticle.ukm.my/8037/ http://journalarticle.ukm.my/8037/1/05_Muhamad_Fahrin.pdf
first_indexed	2023-09-18T19:51:19Z
last_indexed	2023-09-18T19:51:19Z
_version_	1777406238951211008

Antioxidant Activity of Rhodomyrtus tomentosa (Kemunting) Fruits and Its Effect on Lipid Profile in Induced-cholesterol New Zealand White Rabbits

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