Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining

The Piperaceae family comprises about 1000 to 2000 of the species that are widely distributed in tropical and subtropical area. In this study, the ethanol extracts were used to evaluate the cytotoxic activity on human hepatocellular carcinoma (HepG2) and non-malignant Chang’s liver cell lines by usi...

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Main Authors:	Wan Haifa Haryani Wan Omar, Shahrul Hisham Zainal Ariffin, Zaidah Zainal Ariffin, Muhd Fauzi Safian, Sahidan Senafi, Rohaya Megat Abdul Wahab
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2010
Online Access:	http://journalarticle.ukm.my/7437/ http://journalarticle.ukm.my/7437/ http://journalarticle.ukm.my/7437/1/26_Ayiesah.pdf

id	ukm-7437
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spelling	ukm-74372016-12-14T06:44:05Z http://journalarticle.ukm.my/7437/ Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining Wan Haifa Haryani Wan Omar, Shahrul Hisham Zainal Ariffin, Zaidah Zainal Ariffin, Muhd Fauzi Safian, Sahidan Senafi, Rohaya Megat Abdul Wahab, The Piperaceae family comprises about 1000 to 2000 of the species that are widely distributed in tropical and subtropical area. In this study, the ethanol extracts were used to evaluate the cytotoxic activity on human hepatocellular carcinoma (HepG2) and non-malignant Chang’s liver cell lines by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Ethanol extracts from eight Piperaceae families were selected randomly. Results showed that all eight spesies (P. sarmentosum, P. ramifilum, P. paucistigmum, P. betle, P. macronatum, P. ridleyi, P. magnibaccum and P. miniatum) showed cytotoxicity activity with Piper sarmentosum exhibit higher cytotoxicity activity with the IC50 value at 12.5 μg/mL. On the other hand, there were no cytotoxicity activity of Chang’s cell that could be induced by the ethanol extracts because the IC50 values for non-malignant Chang’s cell were greater than 30 μg/mL. Viability analysis using trypan blue showed that P. sarmentosum ethanol extract produced significant (p<0.05) decreased of HepG2 cells as compared to control (p<0.05). In conclusion, all ethanol extracts selected randomly from Piperaceae family showed cytotoxic using MTT assay. Viability analysis using trypan blue staining demonstrates that this type of analysis can become an alternative approach in determining cytotoxicity activity of cells in the presence of extracts. Universiti Kebangsaan Malaysia 2010-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7437/1/26_Ayiesah.pdf Wan Haifa Haryani Wan Omar, and Shahrul Hisham Zainal Ariffin, and Zaidah Zainal Ariffin, and Muhd Fauzi Safian, and Sahidan Senafi, and Rohaya Megat Abdul Wahab, (2010) Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining. Sains Malaysiana, 39 (6). pp. 941-949. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol39num6_2010/contentsVol39num6_2010.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The Piperaceae family comprises about 1000 to 2000 of the species that are widely distributed in tropical and subtropical area. In this study, the ethanol extracts were used to evaluate the cytotoxic activity on human hepatocellular carcinoma (HepG2) and non-malignant Chang’s liver cell lines by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Ethanol extracts from eight Piperaceae families were selected randomly. Results showed that all eight spesies (P. sarmentosum, P. ramifilum, P. paucistigmum, P. betle, P. macronatum, P. ridleyi, P. magnibaccum and P. miniatum) showed cytotoxicity activity with Piper sarmentosum exhibit higher cytotoxicity activity with the IC50 value at 12.5 μg/mL. On the other hand, there were no cytotoxicity activity of Chang’s cell that could be induced by the ethanol extracts because the IC50 values for non-malignant Chang’s cell were greater than 30 μg/mL. Viability analysis using trypan blue showed that P. sarmentosum ethanol extract produced significant (p<0.05) decreased of HepG2 cells as compared to control (p<0.05). In conclusion, all ethanol extracts selected randomly from Piperaceae family showed cytotoxic using MTT assay. Viability analysis using trypan blue staining demonstrates that this type of analysis can become an alternative approach in determining cytotoxicity activity of cells in the presence of extracts.
format	Article
author	Wan Haifa Haryani Wan Omar, Shahrul Hisham Zainal Ariffin, Zaidah Zainal Ariffin, Muhd Fauzi Safian, Sahidan Senafi, Rohaya Megat Abdul Wahab,
spellingShingle	Wan Haifa Haryani Wan Omar, Shahrul Hisham Zainal Ariffin, Zaidah Zainal Ariffin, Muhd Fauzi Safian, Sahidan Senafi, Rohaya Megat Abdul Wahab, Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
author_facet	Wan Haifa Haryani Wan Omar, Shahrul Hisham Zainal Ariffin, Zaidah Zainal Ariffin, Muhd Fauzi Safian, Sahidan Senafi, Rohaya Megat Abdul Wahab,
author_sort	Wan Haifa Haryani Wan Omar,
title	Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
title_short	Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
title_full	Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
title_fullStr	Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
title_full_unstemmed	Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
title_sort	anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining
publisher	Universiti Kebangsaan Malaysia
publishDate	2010
url	http://journalarticle.ukm.my/7437/ http://journalarticle.ukm.my/7437/ http://journalarticle.ukm.my/7437/1/26_Ayiesah.pdf
first_indexed	2023-09-18T19:49:43Z
last_indexed	2023-09-18T19:49:43Z
_version_	1777406138132725760

Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining

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