Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.

The effects of ammonium tartrate and glucose concentration on biomass, lipid and GLA accumulation in Cunninghamella sp. 2A1 were investigated using Response Surface Methodology (RSM). Cultivation was carried out in 250 mL shake flask containing 100 mL of nitrogen limiting medium (with various combin...

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Main Authors:	Farhila Muhid, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff, Othman Omar, Aidil Abdul Hamid
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2010
Online Access:	http://journalarticle.ukm.my/7430/ http://journalarticle.ukm.my/7430/ http://journalarticle.ukm.my/7430/1/26_Ayiesah.pdf

id	ukm-7430
recordtype	eprints
spelling	ukm-74302016-12-14T06:44:03Z http://journalarticle.ukm.my/7430/ Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1. Farhila Muhid, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff, Othman Omar, Aidil Abdul Hamid, The effects of ammonium tartrate and glucose concentration on biomass, lipid and GLA accumulation in Cunninghamella sp. 2A1 were investigated using Response Surface Methodology (RSM). Cultivation was carried out in 250 mL shake flask containing 100 mL of nitrogen limiting medium (with various combinations of concentration of ammonium tartrate (1-3 g/L) and glucose (30-60 g/L) at 30°C and 250 rpm agitation for 120 h. The concentration of both compounds significantly affected the biomass, lipid and GLA yield (p<0.05), with the production of each of them being represented by quadratic models. Higher concentration of ammonium tartrate and glucose (2.99 and 59.33 g/L, respectively) was required for enhanced biomass production whereas low nitrogen content with excess glucose was otherwise favoured for lipid and GLA production. Ammonium tartrate and glucose concentration at 1 and 43 g/L, respectively were estimated by the model and proven to give the highest lipid production and GLA yield of 31.06 % (g/g biomass) and 4.15 ×10-2 (g/g lipid less biomass), respectively. Universiti Kebangsaan Malaysia 2010-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7430/1/26_Ayiesah.pdf Farhila Muhid, and Abdul Jalil Abdul Kader, and Wan Mohtar Wan Yusoff, and Othman Omar, and Aidil Abdul Hamid, (2010) Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1. Sains Malaysiana, 39 (6). pp. 891-899. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol39num6_2010/contentsVol39num6_2010.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The effects of ammonium tartrate and glucose concentration on biomass, lipid and GLA accumulation in Cunninghamella sp. 2A1 were investigated using Response Surface Methodology (RSM). Cultivation was carried out in 250 mL shake flask containing 100 mL of nitrogen limiting medium (with various combinations of concentration of ammonium tartrate (1-3 g/L) and glucose (30-60 g/L) at 30°C and 250 rpm agitation for 120 h. The concentration of both compounds significantly affected the biomass, lipid and GLA yield (p<0.05), with the production of each of them being represented by quadratic models. Higher concentration of ammonium tartrate and glucose (2.99 and 59.33 g/L, respectively) was required for enhanced biomass production whereas low nitrogen content with excess glucose was otherwise favoured for lipid and GLA production. Ammonium tartrate and glucose concentration at 1 and 43 g/L, respectively were estimated by the model and proven to give the highest lipid production and GLA yield of 31.06 % (g/g biomass) and 4.15 ×10-2 (g/g lipid less biomass), respectively.
format	Article
author	Farhila Muhid, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff, Othman Omar, Aidil Abdul Hamid,
spellingShingle	Farhila Muhid, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff, Othman Omar, Aidil Abdul Hamid, Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.
author_facet	Farhila Muhid, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff, Othman Omar, Aidil Abdul Hamid,
author_sort	Farhila Muhid,
title	Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.
title_short	Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.
title_full	Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.
title_fullStr	Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.
title_full_unstemmed	Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.
title_sort	optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by cunninghamella sp. 2a1.
publisher	Universiti Kebangsaan Malaysia
publishDate	2010
url	http://journalarticle.ukm.my/7430/ http://journalarticle.ukm.my/7430/ http://journalarticle.ukm.my/7430/1/26_Ayiesah.pdf
first_indexed	2023-09-18T19:49:42Z
last_indexed	2023-09-18T19:49:42Z
_version_	1777406137108267008

Optimisation of ammonium tartrate and glucose concentration for gamma linolenic acid production by Cunninghamella sp. 2A1.

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