Determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method

Candidiasis infections caused by the fungi Candida spp. are currently the most common systemic fungal infection in hospitalised patients. Diagnostic procedures involving culture or serological tests are either slow or lacking sensitivity and specificity. Thus polymerase chain reaction (PCR) assays w...

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Main Authors:	Jacinta Santhanam, Siti Azura Zainon, Chin, Chook Fung, Faezah Shekh Abdullah
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2010
Online Access:	http://journalarticle.ukm.my/7339/ http://journalarticle.ukm.my/7339/ http://journalarticle.ukm.my/7339/1/01_Md_Yeaminhossain.pdf

id	ukm-7339
recordtype	eprints
spelling	ukm-73392016-12-14T06:43:47Z http://journalarticle.ukm.my/7339/ Determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method Jacinta Santhanam, Siti Azura Zainon, Chin, Chook Fung Faezah Shekh Abdullah, Candidiasis infections caused by the fungi Candida spp. are currently the most common systemic fungal infection in hospitalised patients. Diagnostic procedures involving culture or serological tests are either slow or lacking sensitivity and specificity. Thus polymerase chain reaction (PCR) assays which detect fungal DNA have been developed to provide a rapid, accurate diagnosis. In this study we evaluated a seminested PCR assay for the detection of systemic Candida albicans infection in laboratory animals. Adult Sprague-Dawley rats were infected with C. albicans by intravenous injection of yeast cells. Blood was collected from the animals every 3 to 4 days for extraction of DNA. Each week, 3 animals were sacrificed and their organs were cultured to confirm systemic C. albicans infection. The study period was 4 weeks (28 days). A seminested PCR was performed on DNA samples using universal fungal primers (ITS1 and ITS3) and a C. albicans specific primer (CALB1). The PCR product was detected by agarose gel electrophoresis. The PCR assay was able to detect C. albicans DNA in the blood samples of infected animals consistently from day 2 until day 25 post-infection. Organ culture of kidneys, liver and spleen revealed that the rats were systemically infected until day 21 post-infection and had recovered from infection (negative culture results) by day 28 post-infection. This study shows that the seminested PCR was an effective method to detect systemic C. albicans infection throughout the infection period. Therefore this assay is not only a diagnostic tool but has prognostic value as well for the monitoring of infection status. Universiti Kebangsaan Malaysia 2010-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7339/1/01_Md_Yeaminhossain.pdf Jacinta Santhanam, and Siti Azura Zainon, and Chin, Chook Fung and Faezah Shekh Abdullah, (2010) Determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method. Sains Malaysiana, 39 (2). pp. 321-327. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol39num2_2010/contentsVol39num2_2010.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Candidiasis infections caused by the fungi Candida spp. are currently the most common systemic fungal infection in hospitalised patients. Diagnostic procedures involving culture or serological tests are either slow or lacking sensitivity and specificity. Thus polymerase chain reaction (PCR) assays which detect fungal DNA have been developed to provide a rapid, accurate diagnosis. In this study we evaluated a seminested PCR assay for the detection of systemic Candida albicans infection in laboratory animals. Adult Sprague-Dawley rats were infected with C. albicans by intravenous injection of yeast cells. Blood was collected from the animals every 3 to 4 days for extraction of DNA. Each week, 3 animals were sacrificed and their organs were cultured to confirm systemic C. albicans infection. The study period was 4 weeks (28 days). A seminested PCR was performed on DNA samples using universal fungal primers (ITS1 and ITS3) and a C. albicans specific primer (CALB1). The PCR product was detected by agarose gel electrophoresis. The PCR assay was able to detect C. albicans DNA in the blood samples of infected animals consistently from day 2 until day 25 post-infection. Organ culture of kidneys, liver and spleen revealed that the rats were systemically infected until day 21 post-infection and had recovered from infection (negative culture results) by day 28 post-infection. This study shows that the seminested PCR was an effective method to detect systemic C. albicans infection throughout the infection period. Therefore this assay is not only a diagnostic tool but has prognostic value as well for the monitoring of infection status.
format	Article
author	Jacinta Santhanam, Siti Azura Zainon, Chin, Chook Fung Faezah Shekh Abdullah,
spellingShingle	Jacinta Santhanam, Siti Azura Zainon, Chin, Chook Fung Faezah Shekh Abdullah, Determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method
author_facet	Jacinta Santhanam, Siti Azura Zainon, Chin, Chook Fung Faezah Shekh Abdullah,
author_sort	Jacinta Santhanam,
title	Determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method
title_short	Determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method
title_full	Determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method
title_fullStr	Determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method
title_full_unstemmed	Determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method
title_sort	determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method
publisher	Universiti Kebangsaan Malaysia
publishDate	2010
url	http://journalarticle.ukm.my/7339/ http://journalarticle.ukm.my/7339/ http://journalarticle.ukm.my/7339/1/01_Md_Yeaminhossain.pdf
first_indexed	2023-09-18T19:49:24Z
last_indexed	2023-09-18T19:49:24Z
_version_	1777406118151061504

Determining prognosis of systemic candidiasis infection in rats with the polymerase chain reaction method

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