Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway

Recent studies suggest that reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) may exert beneficial effects on health. Therefore this study evaluated the effects of H2O2 and gamma-tocotrienol (GTT), an antioxidant, on D-[2-3H]Glucose uptake by myotubes compared to insulin. Results showed...

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Main Authors:	Musalmah Mazlan, Rehanna Mansor, Wan Zurinah Wan Ngah
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2010
Online Access:	http://journalarticle.ukm.my/7338/ http://journalarticle.ukm.my/7338/ http://journalarticle.ukm.my/7338/1/01_Md_Yeaminhossain.pdf

id	ukm-7338
recordtype	eprints
spelling	ukm-73382016-12-14T06:43:47Z http://journalarticle.ukm.my/7338/ Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway Musalmah Mazlan, Rehanna Mansor, Wan Zurinah Wan Ngah, Recent studies suggest that reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) may exert beneficial effects on health. Therefore this study evaluated the effects of H2O2 and gamma-tocotrienol (GTT), an antioxidant, on D-[2-3H]Glucose uptake by myotubes compared to insulin. Results showed that H2O2 and insulin significantly increased D-[2-3H]Glucose uptake. This was associated with an increased in Vmax but Michaelis constant (Km) of the transport system remained unchanged indicating that an increase in amount, rather than affinity, of the glucose transporter was involved in the process. Western blot studies confirmed that H2O2 increased the expressions of insulin sensitive glucose transporter, GLUT4 and also hexokinase (an enzyme which phosphorylates glucose and indirectly stimulates glucose uptake by cells). H2O2 and insulin also stimulated increased in PPAR-γ and IRS-1, which are proteins of the insulin transduction pathway. The present data however showed that GTT did not exert any effect on glucose uptake. Thus, the study showed that H2O2 mimicked insulin action in stimulating glucose uptake in myotube cells by influencing the insulin signaling pathway. Universiti Kebangsaan Malaysia 2010 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7338/1/01_Md_Yeaminhossain.pdf Musalmah Mazlan, and Rehanna Mansor, and Wan Zurinah Wan Ngah, (2010) Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway. Sains Malaysiana, 39 (2). pp. 315-320. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol39num2_2010/contentsVol39num2_2010.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Recent studies suggest that reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) may exert beneficial effects on health. Therefore this study evaluated the effects of H2O2 and gamma-tocotrienol (GTT), an antioxidant, on D-[2-3H]Glucose uptake by myotubes compared to insulin. Results showed that H2O2 and insulin significantly increased D-[2-3H]Glucose uptake. This was associated with an increased in Vmax but Michaelis constant (Km) of the transport system remained unchanged indicating that an increase in amount, rather than affinity, of the glucose transporter was involved in the process. Western blot studies confirmed that H2O2 increased the expressions of insulin sensitive glucose transporter, GLUT4 and also hexokinase (an enzyme which phosphorylates glucose and indirectly stimulates glucose uptake by cells). H2O2 and insulin also stimulated increased in PPAR-γ and IRS-1, which are proteins of the insulin transduction pathway. The present data however showed that GTT did not exert any effect on glucose uptake. Thus, the study showed that H2O2 mimicked insulin action in stimulating glucose uptake in myotube cells by influencing the insulin signaling pathway.
format	Article
author	Musalmah Mazlan, Rehanna Mansor, Wan Zurinah Wan Ngah,
spellingShingle	Musalmah Mazlan, Rehanna Mansor, Wan Zurinah Wan Ngah, Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
author_facet	Musalmah Mazlan, Rehanna Mansor, Wan Zurinah Wan Ngah,
author_sort	Musalmah Mazlan,
title	Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
title_short	Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
title_full	Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
title_fullStr	Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
title_full_unstemmed	Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
title_sort	hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
publisher	Universiti Kebangsaan Malaysia
publishDate	2010
url	http://journalarticle.ukm.my/7338/ http://journalarticle.ukm.my/7338/ http://journalarticle.ukm.my/7338/1/01_Md_Yeaminhossain.pdf
first_indexed	2023-09-18T19:49:24Z
last_indexed	2023-09-18T19:49:24Z
_version_	1777406118005309440

Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway

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