Effective hygromycin concentration for selection of Agrobacterium-mediated transgenic Arabidopsis thaliana
The floral-dip transformation method for Arabidopsis thaliana has long been established. Following transformation, an important step is involving the selection for transgenic plants through antibiotics or herbicides. Hygromycin has been widely applied for transgenic plants selection. However, the...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2014
|
| Online Access: | http://journalarticle.ukm.my/7296/ http://journalarticle.ukm.my/7296/ http://journalarticle.ukm.my/7296/1/43_1_13.pdf |
| Summary: | The floral-dip transformation method for Arabidopsis thaliana has long been established. Following transformation, an
important step is involving the selection for transgenic plants through antibiotics or herbicides. Hygromycin has been widely
applied for transgenic plants selection. However, the concentrations used were varied between laboratories mostly in a range
of 10 mg/L up to 200 mg/L. In the present study, the hygromycin sensitivity test was performed on wild-type seeds to obtain
the most effective hygromycin concentration for selection of the transgenic. A standard curve on average mean of hypocotyls
lengths against hygromycin concentrations was constructed. Based on hypocotyls length and leaf colour, the most effective
concentration of hygromycin was determined in the range of 20 to 30 mg/L. For screening the transformed seeds, hygromycin
concentration at 25 mg/L was used. Non-transgenic plants demonstrated a clear decrease in hypocotyls lengths and no root
elongation, as compared to transgenic plants. The identified transgenic seedlings were further verified through polymerase
chain reaction (PCR), giving fragment with an expected size of 365 bp. |
|---|