Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations

Stem cells that made up of embryonic stem cells and adult stem cells are well known for their ability to self renew and differentiate into matured cells. Adult stem cells isolated from peripheral blood system were used as the study sample. The objective of this study was to determine the relationshi...

Full description

Bibliographic Details
Main Authors:	Muniandy, M.K.R., Ruzanna, A.K., Intan Zarina, Z.A., Rohaya, M.A.W., Sahidan, S., Majlis, B.Y., Shahrul Hisham, Z.A.
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/7277/ http://journalarticle.ukm.my/7277/ http://journalarticle.ukm.my/7277/1/42_2_07.pdf

id	ukm-7277
recordtype	eprints
spelling	ukm-72772016-12-14T06:43:36Z http://journalarticle.ukm.my/7277/ Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations Muniandy, M.K.R. Ruzanna, A.K. Intan Zarina, Z.A. Rohaya, M.A.W. Sahidan, S. Majlis, B.Y. Shahrul Hisham, Z.A. Stem cells that made up of embryonic stem cells and adult stem cells are well known for their ability to self renew and differentiate into matured cells. Adult stem cells isolated from peripheral blood system were used as the study sample. The objective of this study was to determine the relationship between size and proliferation potential of stem cells from 3 different culturing terms, i.e., short (7 days), medium (14 days), and long (30 days) terms. Density gradient centrifugation was applied in isolating mononucleated cells (MNCs) from peripheral blood sample. Isolated MNCs were washed with Hank’s Balanced Salt Solution and Phosphate Buffer Saline. The MNCs which cultured in complete media were then subjected to proliferation analysis every day for a time period of 30 days. RT-PCR analyses and cell size measurements were carried out at day 7, 14, and 30. Activation of KIT and SLAMF1 marker genes during RT-PCR analyses indicated that the isolated MNCs were stem cells. Analyses of proliferation rate and cell size showed that short term stem cells have the largest cell size with the lowest proliferation potential. While, medium term stem cells gave rise to a smaller stem cell population with higher proliferation potential compared to short term stem cells. Long term stem cells have smallest cell size with highest proliferation potential. In conclusion, each culturing term cell population had their own sizes and can be isolated based on those sizes. Penerbit Universiti Kebangsaan Malaysia 2013-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7277/1/42_2_07.pdf Muniandy, M.K.R. and Ruzanna, A.K. and Intan Zarina, Z.A. and Rohaya, M.A.W. and Sahidan, S. and Majlis, B.Y. and Shahrul Hisham, Z.A. (2013) Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations. Malaysian Applied Biology, 42 (2). pp. 59-63. ISSN 0126-8643 http://mabjournal.com/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Stem cells that made up of embryonic stem cells and adult stem cells are well known for their ability to self renew and differentiate into matured cells. Adult stem cells isolated from peripheral blood system were used as the study sample. The objective of this study was to determine the relationship between size and proliferation potential of stem cells from 3 different culturing terms, i.e., short (7 days), medium (14 days), and long (30 days) terms. Density gradient centrifugation was applied in isolating mononucleated cells (MNCs) from peripheral blood sample. Isolated MNCs were washed with Hank’s Balanced Salt Solution and Phosphate Buffer Saline. The MNCs which cultured in complete media were then subjected to proliferation analysis every day for a time period of 30 days. RT-PCR analyses and cell size measurements were carried out at day 7, 14, and 30. Activation of KIT and SLAMF1 marker genes during RT-PCR analyses indicated that the isolated MNCs were stem cells. Analyses of proliferation rate and cell size showed that short term stem cells have the largest cell size with the lowest proliferation potential. While, medium term stem cells gave rise to a smaller stem cell population with higher proliferation potential compared to short term stem cells. Long term stem cells have smallest cell size with highest proliferation potential. In conclusion, each culturing term cell population had their own sizes and can be isolated based on those sizes.
format	Article
author	Muniandy, M.K.R. Ruzanna, A.K. Intan Zarina, Z.A. Rohaya, M.A.W. Sahidan, S. Majlis, B.Y. Shahrul Hisham, Z.A.
spellingShingle	Muniandy, M.K.R. Ruzanna, A.K. Intan Zarina, Z.A. Rohaya, M.A.W. Sahidan, S. Majlis, B.Y. Shahrul Hisham, Z.A. Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations
author_facet	Muniandy, M.K.R. Ruzanna, A.K. Intan Zarina, Z.A. Rohaya, M.A.W. Sahidan, S. Majlis, B.Y. Shahrul Hisham, Z.A.
author_sort	Muniandy, M.K.R.
title	Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations
title_short	Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations
title_full	Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations
title_fullStr	Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations
title_full_unstemmed	Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations
title_sort	proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/7277/ http://journalarticle.ukm.my/7277/ http://journalarticle.ukm.my/7277/1/42_2_07.pdf
first_indexed	2023-09-18T19:49:16Z
last_indexed	2023-09-18T19:49:16Z
_version_	1777406109522329600

Proliferation rate and cell size analyses of human peripheral blood suspension stem cells from three culturing terms populations

Similar Items