Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (Mertensiothuria) leucospilota collected from Malaysian waters

This preliminary study aimed to isolate and identify microbes that inhabit the coelomic fluid of two local species of sea cucumbers collected from Malaysian waters - a ‘gamat’ species i.e. Stichopus chloronotus Brandt, 1835 and the most abundant ‘timun laut’ species in Malaysia i.e. Holothuria (Mert...

Full description

Bibliographic Details
Main Authors:	Ahmad Lutfi Lukman, Noor Faizul Hadry Nordin, Kamarul Rahim Kamarudin
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/7256/ http://journalarticle.ukm.my/7256/ http://journalarticle.ukm.my/7256/1/07_Ahmad_Lutfi.pdf

id	ukm-7256
recordtype	eprints
spelling	ukm-72562016-12-14T06:43:32Z http://journalarticle.ukm.my/7256/ Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (Mertensiothuria) leucospilota collected from Malaysian waters Ahmad Lutfi Lukman, Noor Faizul Hadry Nordin, Kamarul Rahim Kamarudin, This preliminary study aimed to isolate and identify microbes that inhabit the coelomic fluid of two local species of sea cucumbers collected from Malaysian waters - a ‘gamat’ species i.e. Stichopus chloronotus Brandt, 1835 and the most abundant ‘timun laut’ species in Malaysia i.e. Holothuria (Mertensiothuria) leucospilota (Brandt 1835). Phylogenetic analyses of partial 16S rRNA mtDNA gene sequences suggested the presence of at least eight microbial genera i.e. five bacterial genera - Bacillus, Exiguobacterium, Pseudomonas, Stenotrophomonas and Vibrio - isolated from the coelomic fluid of H. leucospilota and three genera of gram-positive bacteria from the Micrococcaceae family - Kytococcus, Micrococcus and either Kocuria or Rothia - isolated from the coelomic fluid of S. chloronotus. We speculate that less diverse microbial population in S. chloronotus as compared to H. leucospilota could be due to a number of environmental factors e.g. penetration of light surrounding the habitats of both species, the feeding behaviour of H. leucospilota and the higher level of antimicrobial properties of coelomic fluid in S. chloronotus. In terms of antimicrobial-resistance capability test, an isolate from genus Pseudomonas that is suspected to be P. alcaligenes exhibited high resistance towards streptomycin. Another isolate from genus Stenotrophomonas that was suspected to be S. maltophilia showed moderate resistance towards streptomycin and lower resistance towards kanamycin. Both isolates were from the coelomic fluid of H. leucospilota. Tetracycline inhibited the growth of all bacterial isolates tested. Further studies with more specimens of S. choronotus and H. leucospilota from broader geographical locations and the use of complete mtDNA genes along with morphological approaches for species identification may facilitate to provide better insights into the microbial population in the coelomic fluid of both local sea cucumber species. Universiti Kebangsaan Malaysia 2014-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7256/1/07_Ahmad_Lutfi.pdf Ahmad Lutfi Lukman, and Noor Faizul Hadry Nordin, and Kamarul Rahim Kamarudin, (2014) Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (Mertensiothuria) leucospilota collected from Malaysian waters. Sains Malaysiana, 43 (7). pp. 1013-1021. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This preliminary study aimed to isolate and identify microbes that inhabit the coelomic fluid of two local species of sea cucumbers collected from Malaysian waters - a ‘gamat’ species i.e. Stichopus chloronotus Brandt, 1835 and the most abundant ‘timun laut’ species in Malaysia i.e. Holothuria (Mertensiothuria) leucospilota (Brandt 1835). Phylogenetic analyses of partial 16S rRNA mtDNA gene sequences suggested the presence of at least eight microbial genera i.e. five bacterial genera - Bacillus, Exiguobacterium, Pseudomonas, Stenotrophomonas and Vibrio - isolated from the coelomic fluid of H. leucospilota and three genera of gram-positive bacteria from the Micrococcaceae family - Kytococcus, Micrococcus and either Kocuria or Rothia - isolated from the coelomic fluid of S. chloronotus. We speculate that less diverse microbial population in S. chloronotus as compared to H. leucospilota could be due to a number of environmental factors e.g. penetration of light surrounding the habitats of both species, the feeding behaviour of H. leucospilota and the higher level of antimicrobial properties of coelomic fluid in S. chloronotus. In terms of antimicrobial-resistance capability test, an isolate from genus Pseudomonas that is suspected to be P. alcaligenes exhibited high resistance towards streptomycin. Another isolate from genus Stenotrophomonas that was suspected to be S. maltophilia showed moderate resistance towards streptomycin and lower resistance towards kanamycin. Both isolates were from the coelomic fluid of H. leucospilota. Tetracycline inhibited the growth of all bacterial isolates tested. Further studies with more specimens of S. choronotus and H. leucospilota from broader geographical locations and the use of complete mtDNA genes along with morphological approaches for species identification may facilitate to provide better insights into the microbial population in the coelomic fluid of both local sea cucumber species.
format	Article
author	Ahmad Lutfi Lukman, Noor Faizul Hadry Nordin, Kamarul Rahim Kamarudin,
spellingShingle	Ahmad Lutfi Lukman, Noor Faizul Hadry Nordin, Kamarul Rahim Kamarudin, Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (Mertensiothuria) leucospilota collected from Malaysian waters
author_facet	Ahmad Lutfi Lukman, Noor Faizul Hadry Nordin, Kamarul Rahim Kamarudin,
author_sort	Ahmad Lutfi Lukman,
title	Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (Mertensiothuria) leucospilota collected from Malaysian waters
title_short	Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (Mertensiothuria) leucospilota collected from Malaysian waters
title_full	Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (Mertensiothuria) leucospilota collected from Malaysian waters
title_fullStr	Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (Mertensiothuria) leucospilota collected from Malaysian waters
title_full_unstemmed	Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (Mertensiothuria) leucospilota collected from Malaysian waters
title_sort	microbial population in the coelomic fluid of stichopus chloronotus and holothuria (mertensiothuria) leucospilota collected from malaysian waters
publisher	Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/7256/ http://journalarticle.ukm.my/7256/ http://journalarticle.ukm.my/7256/1/07_Ahmad_Lutfi.pdf
first_indexed	2023-09-18T19:49:10Z
last_indexed	2023-09-18T19:49:10Z
_version_	1777406103594729472

Microbial population in the coelomic fluid of stichopus chloronotus and holothuria (Mertensiothuria) leucospilota collected from Malaysian waters

Similar Items