Direct and indirect plant regenerations of pineapple var. MD2 Ananas comosus L.)
The variety MD2 of pineapple (Ananas comosus L.) was used in this study. This variety is highly demanded in the international market and known for possessing harvest quality with aroma, high sugar content (14% Brix), vitamins and longer shelf life. However, shortage of planting material has limited...
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Penerbit Universiti Kebangsaan Malaysia
2013
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| Online Access: | http://journalarticle.ukm.my/7206/ http://journalarticle.ukm.my/7206/ http://journalarticle.ukm.my/7206/1/42_1_07.pdf |
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ukm-72062016-12-14T06:43:23Z http://journalarticle.ukm.my/7206/ Direct and indirect plant regenerations of pineapple var. MD2 Ananas comosus L.) Hamid, N.S. Bukhori, M.F.M. Jalil, M. The variety MD2 of pineapple (Ananas comosus L.) was used in this study. This variety is highly demanded in the international market and known for possessing harvest quality with aroma, high sugar content (14% Brix), vitamins and longer shelf life. However, shortage of planting material has limited the production in Malaysia. In vitro mass propagation using the direct and indirect shoot proliferation techniques was tested on variety MD2. The plantlets were successfully initiated from sucker on solid MS basal medium containing 30 g/L sucrose, 0.1 g/L Myo-inositol and 3 mg/L BAP after one month of culture. The highest direct shoot tips regeneration was obtained on solid MS medium when added with 30 g/L sucrose, 0.1 g/L Myoinositol,3 mg/L BAP and 1 mg/L NAA. Indirect shoot regeneration was obtained on medium containing 3 mg/L Zeatin after one month of culture. In average, 10 shoots were regenerated from approximately 1 gram of calli. The techniques can produce 100-200 number of plantlet within 4 to 6 months of culture, and ready for planting after 7 months of culture. Penerbit Universiti Kebangsaan Malaysia 2013-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7206/1/42_1_07.pdf Hamid, N.S. and Bukhori, M.F.M. and Jalil, M. (2013) Direct and indirect plant regenerations of pineapple var. MD2 Ananas comosus L.). Malaysian Applied Biology, 42 (1). pp. 61-66. ISSN 0126-8643 http://mabjournal.com/ |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Kebangasaan Malaysia |
| building |
UKM Institutional Repository |
| collection |
Online Access |
| language |
English |
| description |
The variety MD2 of pineapple (Ananas comosus L.) was used in this study. This variety is highly demanded in the international market and known for possessing harvest quality with aroma, high sugar content (14% Brix), vitamins and longer shelf life. However, shortage of planting material has limited the production in Malaysia. In vitro mass propagation using the direct and indirect shoot proliferation techniques was tested on variety MD2. The plantlets were successfully initiated from sucker on
solid MS basal medium containing 30 g/L sucrose, 0.1 g/L Myo-inositol and 3 mg/L BAP after one month of culture. The
highest direct shoot tips regeneration was obtained on solid MS medium when added with 30 g/L sucrose, 0.1 g/L Myoinositol,3 mg/L BAP and 1 mg/L NAA. Indirect shoot regeneration was obtained on medium containing 3 mg/L Zeatin after one month of culture. In average, 10 shoots were regenerated from approximately 1 gram of calli. The techniques can produce 100-200 number of plantlet within 4 to 6 months of culture, and ready for planting after 7 months of culture. |
| format |
Article |
| author |
Hamid, N.S. Bukhori, M.F.M. Jalil, M. |
| spellingShingle |
Hamid, N.S. Bukhori, M.F.M. Jalil, M. Direct and indirect plant regenerations of pineapple var. MD2 Ananas comosus L.) |
| author_facet |
Hamid, N.S. Bukhori, M.F.M. Jalil, M. |
| author_sort |
Hamid, N.S. |
| title |
Direct and indirect plant regenerations of pineapple var. MD2 Ananas comosus L.) |
| title_short |
Direct and indirect plant regenerations of pineapple var. MD2 Ananas comosus L.) |
| title_full |
Direct and indirect plant regenerations of pineapple var. MD2 Ananas comosus L.) |
| title_fullStr |
Direct and indirect plant regenerations of pineapple var. MD2 Ananas comosus L.) |
| title_full_unstemmed |
Direct and indirect plant regenerations of pineapple var. MD2 Ananas comosus L.) |
| title_sort |
direct and indirect plant regenerations of pineapple var. md2 ananas comosus l.) |
| publisher |
Penerbit Universiti Kebangsaan Malaysia |
| publishDate |
2013 |
| url |
http://journalarticle.ukm.my/7206/ http://journalarticle.ukm.my/7206/ http://journalarticle.ukm.my/7206/1/42_1_07.pdf |
| first_indexed |
2023-09-18T19:49:03Z |
| last_indexed |
2023-09-18T19:49:03Z |
| _version_ |
1777406096337534976 |