Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1

Cunninghamella bainieri 2A1 is an oleaginous fungus whose lipid accumulation profile is significantly influenced by metal ion concentrations in growth medium. Mg2+, Fe3+, Mn2+ and Cu2+ were found to be the important elements affecting lipid accumulation in this fungus. This study employs a statistic...

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Main Authors:	Vidyah Manikan, Mohd Sahaid Kalil, Othman Omar, Abdul Jalil Abdul Kader, Aidil Abdul Hamid
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/6931/ http://journalarticle.ukm.my/6931/ http://journalarticle.ukm.my/6931/1/15_Vidyah_Manikan.pdf

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spelling	ukm-69312016-12-14T06:42:37Z http://journalarticle.ukm.my/6931/ Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1 Vidyah Manikan, Mohd Sahaid Kalil, Othman Omar, Abdul Jalil Abdul Kader, Aidil Abdul Hamid, Cunninghamella bainieri 2A1 is an oleaginous fungus whose lipid accumulation profile is significantly influenced by metal ion concentrations in growth medium. Mg2+, Fe3+, Mn2+ and Cu2+ were found to be the important elements affecting lipid accumulation in this fungus. This study employs a statistical method (Response Surface Methodology – RSM) to study the combined effects of Mg2+, Fe3+, Mn2+ and Cu2+ on lipid accumulation of C. baineri 2A1. Cultivation was carried out in 250 mL Erlenmeyer flasks containing 100 mL nitrogen limited medium at 30ºC and 250 rpm agitation for 120 h. A thirty-run central composite design experiment was employed to identify and optimize the significant factors. In addition to Mg2+ and Fe3+ which were shown to have significant effects on lipid accumulation, the interactions between Mg2+ and Cu2+, as well as the effect of Cu2+ in quadratic terms were also found to have significant effect on the process (p<0.05). The highest amount of lipid obtained in this study was 39% g/g biomass with optimal levels of Mg2+, Fe3+ and Cu2+ at 5.00, 0.017 and 0.0005 g/L, respectively, while Mn2+ was omitted. A 32% increment in lipid yield was recorded, where the lipid content increased to 38%, compared to initial yield of 29% g/g biomass prior to optimization. In conclusion, Mg2+ and Fe3+ have significant positive effect on the lipid accumulation of this fungus, whereas Mn2+ and Cu2+ exert negative effects in combination. Universiti Kebangsaan Malaysia 2014-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6931/1/15_Vidyah_Manikan.pdf Vidyah Manikan, and Mohd Sahaid Kalil, and Othman Omar, and Abdul Jalil Abdul Kader, and Aidil Abdul Hamid, (2014) Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1. Sains Malaysiana, 43 (3). pp. 443-449. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Cunninghamella bainieri 2A1 is an oleaginous fungus whose lipid accumulation profile is significantly influenced by metal ion concentrations in growth medium. Mg2+, Fe3+, Mn2+ and Cu2+ were found to be the important elements affecting lipid accumulation in this fungus. This study employs a statistical method (Response Surface Methodology – RSM) to study the combined effects of Mg2+, Fe3+, Mn2+ and Cu2+ on lipid accumulation of C. baineri 2A1. Cultivation was carried out in 250 mL Erlenmeyer flasks containing 100 mL nitrogen limited medium at 30ºC and 250 rpm agitation for 120 h. A thirty-run central composite design experiment was employed to identify and optimize the significant factors. In addition to Mg2+ and Fe3+ which were shown to have significant effects on lipid accumulation, the interactions between Mg2+ and Cu2+, as well as the effect of Cu2+ in quadratic terms were also found to have significant effect on the process (p<0.05). The highest amount of lipid obtained in this study was 39% g/g biomass with optimal levels of Mg2+, Fe3+ and Cu2+ at 5.00, 0.017 and 0.0005 g/L, respectively, while Mn2+ was omitted. A 32% increment in lipid yield was recorded, where the lipid content increased to 38%, compared to initial yield of 29% g/g biomass prior to optimization. In conclusion, Mg2+ and Fe3+ have significant positive effect on the lipid accumulation of this fungus, whereas Mn2+ and Cu2+ exert negative effects in combination.
format	Article
author	Vidyah Manikan, Mohd Sahaid Kalil, Othman Omar, Abdul Jalil Abdul Kader, Aidil Abdul Hamid,
spellingShingle	Vidyah Manikan, Mohd Sahaid Kalil, Othman Omar, Abdul Jalil Abdul Kader, Aidil Abdul Hamid, Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1
author_facet	Vidyah Manikan, Mohd Sahaid Kalil, Othman Omar, Abdul Jalil Abdul Kader, Aidil Abdul Hamid,
author_sort	Vidyah Manikan,
title	Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1
title_short	Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1
title_full	Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1
title_fullStr	Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1
title_full_unstemmed	Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1
title_sort	effects of mg2+, fe3+, mn2+ and cu2+ ions on lipid accumulation by cunninghamella bainieri 2a1
publisher	Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/6931/ http://journalarticle.ukm.my/6931/ http://journalarticle.ukm.my/6931/1/15_Vidyah_Manikan.pdf
first_indexed	2023-09-18T19:48:16Z
last_indexed	2023-09-18T19:48:16Z
_version_	1777406046912905216

Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1

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