In vitro evaluation of biological activities of citrus leaf extracts

Leaves extracts of three different citrus species namely Citrus sinensis (Malta), Citrus paradisi (Grape fruit) and Citrus jambhiri (Khatti) were evaluated for their antioxidant, cytotoxic, antitumor, antimicrobial activities and finally the mineral contents were determined. Four types of extraction...

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Main Authors:	Muhammad Adnan, Atika Umer, Imtiaz Ahmad, Khizar Hayat, Samina N. Shakeel
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/6845/ http://journalarticle.ukm.my/6845/ http://journalarticle.ukm.my/6845/1/04_Muhammad_Adnan.pdf

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recordtype	eprints
spelling	ukm-68452016-12-14T06:42:21Z http://journalarticle.ukm.my/6845/ In vitro evaluation of biological activities of citrus leaf extracts Muhammad Adnan, Atika Umer, Imtiaz Ahmad, Khizar Hayat, Samina N. Shakeel, Leaves extracts of three different citrus species namely Citrus sinensis (Malta), Citrus paradisi (Grape fruit) and Citrus jambhiri (Khatti) were evaluated for their antioxidant, cytotoxic, antitumor, antimicrobial activities and finally the mineral contents were determined. Four types of extraction solvents (100, 80 and 50% methanol and distilled water heated at 50oC) were used for extraction of antioxidant compounds. Extracts yield increased with the elevated levels of aqueous component of organic solvent and our data showed the highest yield in aqueous extracts. All extraction regimes showed in vitro antioxidant activity. Citrus paradisi showed the highest total flavonoid content (TFC), total phenolic content (TPC), reducing power and 1,1-Diphenyl-2- picrylhydrazil free radical scavenging activity in all combinations of solvents followed by Citrus sinensis and Citrus jambhiri. Antioxidant activity was also increased with higher aqueous component of organic solvents in each case. While Citrus sinensis (in 100% methanolic solvent) and Citrus paradisi (in 50% methanolic extract) showed significant cytotoxicity (LD50 values <1000). Antitumor activity was observed in all extracts, however Citrus sinensis and Citrus paradisi (in 50% aqueous methanolic) extracts had the highest antitumor activity from the selected citrus species whereas no antimicrobial activity was observed at higher concentrations (4000 μgmL-1) against specified strains. We found high calcium contents in all three citrus species tested by atomic absorption method. The results showed that the leaves of selected Citrus species possess significant antioxidant, antitumor and cytotoxic activities. Citrus leaves extracts can be potentially helpful in antioxidant protection in food as well as in human body against lipid peroxidation and free radicals damage. It can further be evaluated after in vivo studies using animal models or identifications of high throughput methods for enhanced biological activities. Universiti Kebangsaan Malaysia 2014-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6845/1/04_Muhammad_Adnan.pdf Muhammad Adnan, and Atika Umer, and Imtiaz Ahmad, and Khizar Hayat, and Samina N. Shakeel, (2014) In vitro evaluation of biological activities of citrus leaf extracts. Sains Malaysiana, 43 (2). pp. 185-194. ISSN 0126-6039 http://www.ukm.my/jsm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Leaves extracts of three different citrus species namely Citrus sinensis (Malta), Citrus paradisi (Grape fruit) and Citrus jambhiri (Khatti) were evaluated for their antioxidant, cytotoxic, antitumor, antimicrobial activities and finally the mineral contents were determined. Four types of extraction solvents (100, 80 and 50% methanol and distilled water heated at 50oC) were used for extraction of antioxidant compounds. Extracts yield increased with the elevated levels of aqueous component of organic solvent and our data showed the highest yield in aqueous extracts. All extraction regimes showed in vitro antioxidant activity. Citrus paradisi showed the highest total flavonoid content (TFC), total phenolic content (TPC), reducing power and 1,1-Diphenyl-2- picrylhydrazil free radical scavenging activity in all combinations of solvents followed by Citrus sinensis and Citrus jambhiri. Antioxidant activity was also increased with higher aqueous component of organic solvents in each case. While Citrus sinensis (in 100% methanolic solvent) and Citrus paradisi (in 50% methanolic extract) showed significant cytotoxicity (LD50 values <1000). Antitumor activity was observed in all extracts, however Citrus sinensis and Citrus paradisi (in 50% aqueous methanolic) extracts had the highest antitumor activity from the selected citrus species whereas no antimicrobial activity was observed at higher concentrations (4000 μgmL-1) against specified strains. We found high calcium contents in all three citrus species tested by atomic absorption method. The results showed that the leaves of selected Citrus species possess significant antioxidant, antitumor and cytotoxic activities. Citrus leaves extracts can be potentially helpful in antioxidant protection in food as well as in human body against lipid peroxidation and free radicals damage. It can further be evaluated after in vivo studies using animal models or identifications of high throughput methods for enhanced biological activities.
format	Article
author	Muhammad Adnan, Atika Umer, Imtiaz Ahmad, Khizar Hayat, Samina N. Shakeel,
spellingShingle	Muhammad Adnan, Atika Umer, Imtiaz Ahmad, Khizar Hayat, Samina N. Shakeel, In vitro evaluation of biological activities of citrus leaf extracts
author_facet	Muhammad Adnan, Atika Umer, Imtiaz Ahmad, Khizar Hayat, Samina N. Shakeel,
author_sort	Muhammad Adnan,
title	In vitro evaluation of biological activities of citrus leaf extracts
title_short	In vitro evaluation of biological activities of citrus leaf extracts
title_full	In vitro evaluation of biological activities of citrus leaf extracts
title_fullStr	In vitro evaluation of biological activities of citrus leaf extracts
title_full_unstemmed	In vitro evaluation of biological activities of citrus leaf extracts
title_sort	in vitro evaluation of biological activities of citrus leaf extracts
publisher	Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/6845/ http://journalarticle.ukm.my/6845/ http://journalarticle.ukm.my/6845/1/04_Muhammad_Adnan.pdf
first_indexed	2023-09-18T19:48:02Z
last_indexed	2023-09-18T19:48:02Z
_version_	1777406031612084224

In vitro evaluation of biological activities of citrus leaf extracts

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