Comparison of biofilms morphology for two strains of marine sulfate-reducing bacteria using atomic force microscopy

AISI 304 stainless steel coupons were immersed in VMNI medium containing two strains of Sulfate-reducing bacteria which were isolated from Port Dickson, Negeri Sembilan. The immersion period were set for twenty-four hour, seven days, nine days and fifteen days. At the end of each period, the biofilm...

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Main Authors:	Nawawi, M.F., Sahrani, F.K., Usup, G., Ahmad, A., Wan Muda, W.M.L.
Format:	Article
Language:	English
Published:	University Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/6803/ http://journalarticle.ukm.my/6803/ http://journalarticle.ukm.my/6803/1/35-64-1-SM.pdf

id	ukm-6803
recordtype	eprints
spelling	ukm-68032016-12-14T06:42:14Z http://journalarticle.ukm.my/6803/ Comparison of biofilms morphology for two strains of marine sulfate-reducing bacteria using atomic force microscopy Nawawi, M.F. Sahrani, F.K. Usup, G. Ahmad, A. Wan Muda, W.M.L. AISI 304 stainless steel coupons were immersed in VMNI medium containing two strains of Sulfate-reducing bacteria which were isolated from Port Dickson, Negeri Sembilan. The immersion period were set for twenty-four hour, seven days, nine days and fifteen days. At the end of each period, the biofilm were formed on the surface will be maps using Atomic Force Microscopy. The image were taken using contact mode in the 70 μm x 70 μm and 10 μm x 10 μm resolution. Bacteria counts were done using the dilution and plate count technique. Image showed that SRB2 contain more bacteria cell attached to the metal surface by the 7th day of immersion compare to SRB1. But SRB1 produce more condensed and slime like biofilm with some crystallization precipitation while SRB2 bacteria cell were loosely attached. SRB1 bacteria cell were engulf in the slime like matrix while SRB2 bacteria cell is densely packed together. SRB2 showed a slightly higher rate growth in the medium compared to SRB1 but produce low amount of biofilm matrix while SRB1 produce a lot of bio-matrix precipitation and slime formation. University Kebangsaan Malaysia 2013 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6803/1/35-64-1-SM.pdf Nawawi, M.F. and Sahrani, F.K. and Usup, G. and Ahmad, A. and Wan Muda, W.M.L. (2013) Comparison of biofilms morphology for two strains of marine sulfate-reducing bacteria using atomic force microscopy. Journal of Tropical Marine Ecosystem, 3 (1). pp. 28-39. ISSN 2231-8062 http://www.ukm.my/jtme/index.php
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	AISI 304 stainless steel coupons were immersed in VMNI medium containing two strains of Sulfate-reducing bacteria which were isolated from Port Dickson, Negeri Sembilan. The immersion period were set for twenty-four hour, seven days, nine days and fifteen days. At the end of each period, the biofilm were formed on the surface will be maps using Atomic Force Microscopy. The image were taken using contact mode in the 70 μm x 70 μm and 10 μm x 10 μm resolution. Bacteria counts were done using the dilution and plate count technique. Image showed that SRB2 contain more bacteria cell attached to the metal surface by the 7th day of immersion compare to SRB1. But SRB1 produce more condensed and slime like biofilm with some crystallization precipitation while SRB2 bacteria cell were loosely attached. SRB1 bacteria cell were engulf in the slime like matrix while SRB2 bacteria cell is densely packed together. SRB2 showed a slightly higher rate growth in the medium compared to SRB1 but produce low amount of biofilm matrix while SRB1 produce a lot of bio-matrix precipitation and slime formation.
format	Article
author	Nawawi, M.F. Sahrani, F.K. Usup, G. Ahmad, A. Wan Muda, W.M.L.
spellingShingle	Nawawi, M.F. Sahrani, F.K. Usup, G. Ahmad, A. Wan Muda, W.M.L. Comparison of biofilms morphology for two strains of marine sulfate-reducing bacteria using atomic force microscopy
author_facet	Nawawi, M.F. Sahrani, F.K. Usup, G. Ahmad, A. Wan Muda, W.M.L.
author_sort	Nawawi, M.F.
title	Comparison of biofilms morphology for two strains of marine sulfate-reducing bacteria using atomic force microscopy
title_short	Comparison of biofilms morphology for two strains of marine sulfate-reducing bacteria using atomic force microscopy
title_full	Comparison of biofilms morphology for two strains of marine sulfate-reducing bacteria using atomic force microscopy
title_fullStr	Comparison of biofilms morphology for two strains of marine sulfate-reducing bacteria using atomic force microscopy
title_full_unstemmed	Comparison of biofilms morphology for two strains of marine sulfate-reducing bacteria using atomic force microscopy
title_sort	comparison of biofilms morphology for two strains of marine sulfate-reducing bacteria using atomic force microscopy
publisher	University Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/6803/ http://journalarticle.ukm.my/6803/ http://journalarticle.ukm.my/6803/1/35-64-1-SM.pdf
first_indexed	2023-09-18T19:47:56Z
last_indexed	2023-09-18T19:47:56Z
_version_	1777406026059874304

Comparison of biofilms morphology for two strains of marine sulfate-reducing bacteria using atomic force microscopy

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