Processing optimization and characterization of gelatin from catfish (Clarias gariepinus) skin

The extraction of catfish (Clarias gariepinus) skin gelatin was optimized by using response surface methodology (RSM) employing a central composite design (CCD). RSM with 3-factors, 5-levels CCD was carried out for the optimization. The independent variables were suggested which include NaOH concent...

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Main Authors: Ardekani, V. Sanaei, Mahmoodani, F., See, S.F., Yusop, S.M., Babji, A.S.
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2013
Online Access:http://journalarticle.ukm.my/6678/
http://journalarticle.ukm.my/6678/
http://journalarticle.ukm.my/6678/1/02_Sanaei.pdf
id ukm-6678
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spelling ukm-66782016-12-14T06:41:53Z http://journalarticle.ukm.my/6678/ Processing optimization and characterization of gelatin from catfish (Clarias gariepinus) skin Ardekani, V. Sanaei Mahmoodani, F. See, S.F. Yusop, S.M. Babji, A.S. The extraction of catfish (Clarias gariepinus) skin gelatin was optimized by using response surface methodology (RSM) employing a central composite design (CCD). RSM with 3-factors, 5-levels CCD was carried out for the optimization. The independent variables were suggested which include NaOH concentration (0.07-0.23 mol/L), acetic acid concentration (0.04-0.14 mol/L) and extraction temperature (40-80°C) with the percentage of hydroxyproline recovery (Y) as dependant variable. A maximum Y of 65.32% for gelatin processing was obtained using a combination of 0.13 mol/L NaOH and 0.09 mol/L acetic acid for 1 h, followed by a hot-water extraction at 64.92°C for 3 h. The results indicated a high protein content (88.46 g/100 g) in the extracted gelatin with a viscosity of 3.45 mPa.s, 286.71 g gel strength and 173 residues per 1000 residues of imino acids (proline and hydroxyproline). Furthermore, the gelatin from catfish also showed a relatively good instrumental texture quality according to texture profile analysis (TPA). Universiti Kebangsaan Malaysia 2013-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6678/1/02_Sanaei.pdf Ardekani, V. Sanaei and Mahmoodani, F. and See, S.F. and Yusop, S.M. and Babji, A.S. (2013) Processing optimization and characterization of gelatin from catfish (Clarias gariepinus) skin. Sains Malaysiana, 42 (12). pp. 1697-1705. ISSN 0126-6039 http://www.ukm.my/jsm/
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description The extraction of catfish (Clarias gariepinus) skin gelatin was optimized by using response surface methodology (RSM) employing a central composite design (CCD). RSM with 3-factors, 5-levels CCD was carried out for the optimization. The independent variables were suggested which include NaOH concentration (0.07-0.23 mol/L), acetic acid concentration (0.04-0.14 mol/L) and extraction temperature (40-80°C) with the percentage of hydroxyproline recovery (Y) as dependant variable. A maximum Y of 65.32% for gelatin processing was obtained using a combination of 0.13 mol/L NaOH and 0.09 mol/L acetic acid for 1 h, followed by a hot-water extraction at 64.92°C for 3 h. The results indicated a high protein content (88.46 g/100 g) in the extracted gelatin with a viscosity of 3.45 mPa.s, 286.71 g gel strength and 173 residues per 1000 residues of imino acids (proline and hydroxyproline). Furthermore, the gelatin from catfish also showed a relatively good instrumental texture quality according to texture profile analysis (TPA).
format Article
author Ardekani, V. Sanaei
Mahmoodani, F.
See, S.F.
Yusop, S.M.
Babji, A.S.
spellingShingle Ardekani, V. Sanaei
Mahmoodani, F.
See, S.F.
Yusop, S.M.
Babji, A.S.
Processing optimization and characterization of gelatin from catfish (Clarias gariepinus) skin
author_facet Ardekani, V. Sanaei
Mahmoodani, F.
See, S.F.
Yusop, S.M.
Babji, A.S.
author_sort Ardekani, V. Sanaei
title Processing optimization and characterization of gelatin from catfish (Clarias gariepinus) skin
title_short Processing optimization and characterization of gelatin from catfish (Clarias gariepinus) skin
title_full Processing optimization and characterization of gelatin from catfish (Clarias gariepinus) skin
title_fullStr Processing optimization and characterization of gelatin from catfish (Clarias gariepinus) skin
title_full_unstemmed Processing optimization and characterization of gelatin from catfish (Clarias gariepinus) skin
title_sort processing optimization and characterization of gelatin from catfish (clarias gariepinus) skin
publisher Universiti Kebangsaan Malaysia
publishDate 2013
url http://journalarticle.ukm.my/6678/
http://journalarticle.ukm.my/6678/
http://journalarticle.ukm.my/6678/1/02_Sanaei.pdf
first_indexed 2023-09-18T19:47:34Z
last_indexed 2023-09-18T19:47:34Z
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