Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells

Salted fish is a locally processed raw food which is used in everyday cooking among Malaysians. Previous studies suggested that salted fish intake was a risk of nasopharyngeal cancer. Hence, this study was carried out to evaluate gene mutation effects through the induction of mutagenic effect of aqu...

Full description

Bibliographic Details
Main Authors:	Ahmad Rohi Ghazali, Rasyidah Mohamad Halim, Nor Fadilah Rajab, Noorain Ramli, Rozaini Abdullah, Firdaus Kamarulzaman, Zaliha Harun
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/6624/ http://journalarticle.ukm.my/6624/ http://journalarticle.ukm.my/6624/1/09_Ahmad_Rohi_Ghazali-1.pdf

id	ukm-6624
recordtype	eprints
spelling	ukm-66242016-12-14T06:41:43Z http://journalarticle.ukm.my/6624/ Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells Ahmad Rohi Ghazali, Rasyidah Mohamad Halim, Nor Fadilah Rajab, Noorain Ramli, Rozaini Abdullah, Firdaus Kamarulzaman, Zaliha Harun, Salted fish is a locally processed raw food which is used in everyday cooking among Malaysians. Previous studies suggested that salted fish intake was a risk of nasopharyngeal cancer. Hence, this study was carried out to evaluate gene mutation effects through the induction of mutagenic effect of aqueous and methanol extracts of salted fish from Balik Pulau, Pulau Pinang, Malaysia. Balik Pulau was chosen for sampling purpose due to its popularity as a commercial centre for local raw fisheries in Malaysia. Evaluation of mutagenic effect was carried out by hprt Gene Mutation Assay towards V79 lung fibroblast cells. It was found that the aqueous and methanol extracts of salted fish were not cytotoxic towards V79 lung fibroblast cells. It was also found that the extracts of salted fish from Balik Pulau were not mutagenic towards hprt gene of V79 lung fibroblast cells as the mutation frequency of the extracts did not exceed 3 times of the value for negative control mutation frequency. In conclusion, both aqueous and methanol extracts of salted fish from Balik Pulau did not have gene mutation effect towards hprt gene in vitro. However, other toxicological profile could be assessed to determine the mechanism of toxicity of salted fish. Universiti Kebangsaan Malaysia 2013-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6624/1/09_Ahmad_Rohi_Ghazali-1.pdf Ahmad Rohi Ghazali, and Rasyidah Mohamad Halim, and Nor Fadilah Rajab, and Noorain Ramli, and Rozaini Abdullah, and Firdaus Kamarulzaman, and Zaliha Harun, (2013) Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells. Sains Malaysiana, 42 (11). pp. 1599-1603. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Salted fish is a locally processed raw food which is used in everyday cooking among Malaysians. Previous studies suggested that salted fish intake was a risk of nasopharyngeal cancer. Hence, this study was carried out to evaluate gene mutation effects through the induction of mutagenic effect of aqueous and methanol extracts of salted fish from Balik Pulau, Pulau Pinang, Malaysia. Balik Pulau was chosen for sampling purpose due to its popularity as a commercial centre for local raw fisheries in Malaysia. Evaluation of mutagenic effect was carried out by hprt Gene Mutation Assay towards V79 lung fibroblast cells. It was found that the aqueous and methanol extracts of salted fish were not cytotoxic towards V79 lung fibroblast cells. It was also found that the extracts of salted fish from Balik Pulau were not mutagenic towards hprt gene of V79 lung fibroblast cells as the mutation frequency of the extracts did not exceed 3 times of the value for negative control mutation frequency. In conclusion, both aqueous and methanol extracts of salted fish from Balik Pulau did not have gene mutation effect towards hprt gene in vitro. However, other toxicological profile could be assessed to determine the mechanism of toxicity of salted fish.
format	Article
author	Ahmad Rohi Ghazali, Rasyidah Mohamad Halim, Nor Fadilah Rajab, Noorain Ramli, Rozaini Abdullah, Firdaus Kamarulzaman, Zaliha Harun,
spellingShingle	Ahmad Rohi Ghazali, Rasyidah Mohamad Halim, Nor Fadilah Rajab, Noorain Ramli, Rozaini Abdullah, Firdaus Kamarulzaman, Zaliha Harun, Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells
author_facet	Ahmad Rohi Ghazali, Rasyidah Mohamad Halim, Nor Fadilah Rajab, Noorain Ramli, Rozaini Abdullah, Firdaus Kamarulzaman, Zaliha Harun,
author_sort	Ahmad Rohi Ghazali,
title	Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells
title_short	Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells
title_full	Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells
title_fullStr	Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells
title_full_unstemmed	Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells
title_sort	gene mutation effect of aqueous and methanol extracts of salted fish from pulau pinang, malaysia towards v79 lung fibroblast cells
publisher	Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/6624/ http://journalarticle.ukm.my/6624/ http://journalarticle.ukm.my/6624/1/09_Ahmad_Rohi_Ghazali-1.pdf
first_indexed	2023-09-18T19:47:27Z
last_indexed	2023-09-18T19:47:27Z
_version_	1777405995150999552

Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells

Similar Items