Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells

In this study, RNA interference (RNAi) was carried out as an experimental technique to knockdown three mitogen-activated protein kinase (MAPK) pathway genes, raf-1, mekk1 and mlk3 in acute myeloid leukemia (AML) cells. Conventionally, RNAi knockdown experiments target a single gene for functional st...

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Main Authors:	Mohd Hafiz, M.R., Mazatulikhma, M.Z., Mohd Faiz, F.A., Mohamed Saifulaman, M.S.
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/6452/ http://journalarticle.ukm.my/6452/ http://journalarticle.ukm.my/6452/1/13_M.R._Mohd_Hafiz.pdf

id	ukm-6452
recordtype	eprints
spelling	ukm-64522016-12-14T06:41:13Z http://journalarticle.ukm.my/6452/ Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells Mohd Hafiz, M.R. Mazatulikhma, M.Z. Mohd Faiz, F.A. Mohamed Saifulaman, M.S. In this study, RNA interference (RNAi) was carried out as an experimental technique to knockdown three mitogen-activated protein kinase (MAPK) pathway genes, raf-1, mekk1 and mlk3 in acute myeloid leukemia (AML) cells. Conventionally, RNAi knockdown experiments target a single gene for functional studies or therapeutic purposes. We wanted to explore the potential differences or similarities between targeting single targets or multiple target genes in a single application. We achieved knockdown of gene expression levels of between 40 and 60% for the RNAi experiments, with better knockdown observed in single target gene experiments in comparison with the multiple target gene experiment. Microarray analysis indicated that the transfection process had most likely induced the immune response from the cells in every RNAi treatment. This might indicate that when the MAPK signaling pathway is partially blocked, in tandem with the immune response, the cells will begin signaling for apoptosis leading to cellular death of the leukemic cells. Universiti Kebangsaan Malaysia 2013-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6452/1/13_M.R._Mohd_Hafiz.pdf Mohd Hafiz, M.R. and Mazatulikhma, M.Z. and Mohd Faiz, F.A. and Mohamed Saifulaman, M.S. (2013) Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells. Sains Malaysiana, 42 (8). pp. 1131-1137. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In this study, RNA interference (RNAi) was carried out as an experimental technique to knockdown three mitogen-activated protein kinase (MAPK) pathway genes, raf-1, mekk1 and mlk3 in acute myeloid leukemia (AML) cells. Conventionally, RNAi knockdown experiments target a single gene for functional studies or therapeutic purposes. We wanted to explore the potential differences or similarities between targeting single targets or multiple target genes in a single application. We achieved knockdown of gene expression levels of between 40 and 60% for the RNAi experiments, with better knockdown observed in single target gene experiments in comparison with the multiple target gene experiment. Microarray analysis indicated that the transfection process had most likely induced the immune response from the cells in every RNAi treatment. This might indicate that when the MAPK signaling pathway is partially blocked, in tandem with the immune response, the cells will begin signaling for apoptosis leading to cellular death of the leukemic cells.
format	Article
author	Mohd Hafiz, M.R. Mazatulikhma, M.Z. Mohd Faiz, F.A. Mohamed Saifulaman, M.S.
spellingShingle	Mohd Hafiz, M.R. Mazatulikhma, M.Z. Mohd Faiz, F.A. Mohamed Saifulaman, M.S. Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells
author_facet	Mohd Hafiz, M.R. Mazatulikhma, M.Z. Mohd Faiz, F.A. Mohamed Saifulaman, M.S.
author_sort	Mohd Hafiz, M.R.
title	Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells
title_short	Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells
title_full	Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells
title_fullStr	Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells
title_full_unstemmed	Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells
title_sort	targeted rnai of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells
publisher	Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/6452/ http://journalarticle.ukm.my/6452/ http://journalarticle.ukm.my/6452/1/13_M.R._Mohd_Hafiz.pdf
first_indexed	2023-09-18T19:46:55Z
last_indexed	2023-09-18T19:46:55Z
_version_	1777405962153361408

Targeted RNAi of the mitogen-activated protein kinase pathway genes in acute myeloid leukemia cells

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