Screening and optimization of biosurfactant production by the hydrocarbon-degrading bacteria

Biosurfactants are amphiphilic compounds produced by microorganisms as secondary metabolite. The unique properties of biosurfactants make them possible to replace or to be added to synthetic surfactants which are mainly used in food, cosmetics and pharmaceutical industries and in environmental appli...

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Main Authors:	Ainon Hamzah, Noramiza Sabturani, Shahidan Radiman
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/6154/ http://journalarticle.ukm.my/6154/ http://journalarticle.ukm.my/6154/1/08_Ainon_Hamzah.pdf

id	ukm-6154
recordtype	eprints
spelling	ukm-61542016-12-14T06:40:30Z http://journalarticle.ukm.my/6154/ Screening and optimization of biosurfactant production by the hydrocarbon-degrading bacteria Ainon Hamzah, Noramiza Sabturani, Shahidan Radiman, Biosurfactants are amphiphilic compounds produced by microorganisms as secondary metabolite. The unique properties of biosurfactants make them possible to replace or to be added to synthetic surfactants which are mainly used in food, cosmetics and pharmaceutical industries and in environmental applications. In this study twenty hydrocarbon-degrading bacteria were screened for biosurfactant production. All of the bacterial isolates were grown in mineral salt medium (MSM) with addition of 1% (v/v) Tapis crude oil as carbon source. The presence of biosurfactant was determined by the drop-collapse test, microplate analysis, oil spreading technique, emulsification index (%EI24) and surface tension measurement. Only one isolate, Pseudomonas aeruginosa UKMP14T, was found to be positive for all the qualitative tests and reducing the surface tension of the medium to 49.5 dynes/cm with emulsification index of 25.29%. This isolate produced biosurfactant optimally at pH9.0 and incubation temperature of 37°C. Furthermore, P. aeruginosa UKMP14T when grown in MSM with addition of 1% (v/v) glycerol and 1.3 g/L ammonium sulphate with C/N ratio 14:1 produced biosurfactant with percentage of surface tension reduction at 55% or 30.6 dynes/cm with %EI24 of 43%. This percentage of surface tension reduction represents an increasing reduction in surface tension of medium by 39% over the value before optimization. This study showed that P. aeruginosa UKMP14T has the ability to biodegrade hydrocarbon and concurrently produce biosurfactant. Universiti Kebangsaan Malaysia 2013-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6154/1/08_Ainon_Hamzah.pdf Ainon Hamzah, and Noramiza Sabturani, and Shahidan Radiman, (2013) Screening and optimization of biosurfactant production by the hydrocarbon-degrading bacteria. Sains Malaysiana, 42 (5). pp. 615-623. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Biosurfactants are amphiphilic compounds produced by microorganisms as secondary metabolite. The unique properties of biosurfactants make them possible to replace or to be added to synthetic surfactants which are mainly used in food, cosmetics and pharmaceutical industries and in environmental applications. In this study twenty hydrocarbon-degrading bacteria were screened for biosurfactant production. All of the bacterial isolates were grown in mineral salt medium (MSM) with addition of 1% (v/v) Tapis crude oil as carbon source. The presence of biosurfactant was determined by the drop-collapse test, microplate analysis, oil spreading technique, emulsification index (%EI24) and surface tension measurement. Only one isolate, Pseudomonas aeruginosa UKMP14T, was found to be positive for all the qualitative tests and reducing the surface tension of the medium to 49.5 dynes/cm with emulsification index of 25.29%. This isolate produced biosurfactant optimally at pH9.0 and incubation temperature of 37°C. Furthermore, P. aeruginosa UKMP14T when grown in MSM with addition of 1% (v/v) glycerol and 1.3 g/L ammonium sulphate with C/N ratio 14:1 produced biosurfactant with percentage of surface tension reduction at 55% or 30.6 dynes/cm with %EI24 of 43%. This percentage of surface tension reduction represents an increasing reduction in surface tension of medium by 39% over the value before optimization. This study showed that P. aeruginosa UKMP14T has the ability to biodegrade hydrocarbon and concurrently produce biosurfactant.
format	Article
author	Ainon Hamzah, Noramiza Sabturani, Shahidan Radiman,
spellingShingle	Ainon Hamzah, Noramiza Sabturani, Shahidan Radiman, Screening and optimization of biosurfactant production by the hydrocarbon-degrading bacteria
author_facet	Ainon Hamzah, Noramiza Sabturani, Shahidan Radiman,
author_sort	Ainon Hamzah,
title	Screening and optimization of biosurfactant production by the hydrocarbon-degrading bacteria
title_short	Screening and optimization of biosurfactant production by the hydrocarbon-degrading bacteria
title_full	Screening and optimization of biosurfactant production by the hydrocarbon-degrading bacteria
title_fullStr	Screening and optimization of biosurfactant production by the hydrocarbon-degrading bacteria
title_full_unstemmed	Screening and optimization of biosurfactant production by the hydrocarbon-degrading bacteria
title_sort	screening and optimization of biosurfactant production by the hydrocarbon-degrading bacteria
publisher	Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/6154/ http://journalarticle.ukm.my/6154/ http://journalarticle.ukm.my/6154/1/08_Ainon_Hamzah.pdf
first_indexed	2023-09-18T19:46:08Z
last_indexed	2023-09-18T19:46:08Z
_version_	1777405911998922752

Screening and optimization of biosurfactant production by the hydrocarbon-degrading bacteria

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