Evaluation of antimicrobial efficacy of antibiotics and calcium hydroxide against enterococcus faecalis biofilm in dentine

The objective of this study was to investigate the antimicrobial efficacy of erythromycin, oxytetracycline and calcium hydroxide [Ca(OH)2] against Enterococcus faecalis biofilm in dentine. E. faecalis ATCC 29212 (American type culture collection) was inoculated into standard tooth sections and incub...

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Main Authors:	Chai, W.L, Hamimah, H, Hamimah, M
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/5707/ http://journalarticle.ukm.my/5707/ http://journalarticle.ukm.my/5707/1/12%2520W.I.%2520Chai.pdf

id	ukm-5707
recordtype	eprints
spelling	ukm-57072016-12-14T06:39:16Z http://journalarticle.ukm.my/5707/ Evaluation of antimicrobial efficacy of antibiotics and calcium hydroxide against enterococcus faecalis biofilm in dentine Chai, W.L Hamimah, H Hamimah, M The objective of this study was to investigate the antimicrobial efficacy of erythromycin, oxytetracycline and calcium hydroxide [Ca(OH)2] against Enterococcus faecalis biofilm in dentine. E. faecalis ATCC 29212 (American type culture collection) was inoculated into standard tooth sections and incubated in aerobic atmosphere at 37°C for 21 days. The infected tooth sections were then exposed to the test agents for 5 and 10 min. The colony forming units (CFU) after the exposure periods at three different depths <100 μm, 100-350 μm and 350-500 μm were enumerated. After 5 min of exposure, both antibiotics had significantly lower CFU count than Ca(OH)2 solution at three dentinal depths. Comparing with the oxytetracycline, the CFU count of the erythromycin was significantly (p<0.05) lower at the depth of 100-500 μm. Similarly, after 10 min of exposure, erythromycin had significantly lower CFU count (p<0.05) at three dentinal depths. Oxytetracycline showed significantly lower CFU count than Ca(OH)2 at 100 μm depth. Comparing with the two exposure times, the erythromycin and Ca(OH)2 groups showed significant lower CFU counts after 10 min of exposure in the antimicrobial agents to 5 min. In conclusion, both antibiotics show better antimicrobial activity than Ca(OH)2 in removing the E. faecalis biofilm in dentine. Universiti Kebangsaan Malaysia 2013-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/5707/1/12%2520W.I.%2520Chai.pdf Chai, W.L and Hamimah, H and Hamimah, M (2013) Evaluation of antimicrobial efficacy of antibiotics and calcium hydroxide against enterococcus faecalis biofilm in dentine. Sains Malaysiana, 42 (1). pp. 73-80. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The objective of this study was to investigate the antimicrobial efficacy of erythromycin, oxytetracycline and calcium hydroxide [Ca(OH)2] against Enterococcus faecalis biofilm in dentine. E. faecalis ATCC 29212 (American type culture collection) was inoculated into standard tooth sections and incubated in aerobic atmosphere at 37°C for 21 days. The infected tooth sections were then exposed to the test agents for 5 and 10 min. The colony forming units (CFU) after the exposure periods at three different depths <100 μm, 100-350 μm and 350-500 μm were enumerated. After 5 min of exposure, both antibiotics had significantly lower CFU count than Ca(OH)2 solution at three dentinal depths. Comparing with the oxytetracycline, the CFU count of the erythromycin was significantly (p<0.05) lower at the depth of 100-500 μm. Similarly, after 10 min of exposure, erythromycin had significantly lower CFU count (p<0.05) at three dentinal depths. Oxytetracycline showed significantly lower CFU count than Ca(OH)2 at 100 μm depth. Comparing with the two exposure times, the erythromycin and Ca(OH)2 groups showed significant lower CFU counts after 10 min of exposure in the antimicrobial agents to 5 min. In conclusion, both antibiotics show better antimicrobial activity than Ca(OH)2 in removing the E. faecalis biofilm in dentine.
format	Article
author	Chai, W.L Hamimah, H Hamimah, M
spellingShingle	Chai, W.L Hamimah, H Hamimah, M Evaluation of antimicrobial efficacy of antibiotics and calcium hydroxide against enterococcus faecalis biofilm in dentine
author_facet	Chai, W.L Hamimah, H Hamimah, M
author_sort	Chai, W.L
title	Evaluation of antimicrobial efficacy of antibiotics and calcium hydroxide against enterococcus faecalis biofilm in dentine
title_short	Evaluation of antimicrobial efficacy of antibiotics and calcium hydroxide against enterococcus faecalis biofilm in dentine
title_full	Evaluation of antimicrobial efficacy of antibiotics and calcium hydroxide against enterococcus faecalis biofilm in dentine
title_fullStr	Evaluation of antimicrobial efficacy of antibiotics and calcium hydroxide against enterococcus faecalis biofilm in dentine
title_full_unstemmed	Evaluation of antimicrobial efficacy of antibiotics and calcium hydroxide against enterococcus faecalis biofilm in dentine
title_sort	evaluation of antimicrobial efficacy of antibiotics and calcium hydroxide against enterococcus faecalis biofilm in dentine
publisher	Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/5707/ http://journalarticle.ukm.my/5707/ http://journalarticle.ukm.my/5707/1/12%2520W.I.%2520Chai.pdf
first_indexed	2023-09-18T19:44:53Z
last_indexed	2023-09-18T19:44:53Z
_version_	1777405834021568512

Evaluation of antimicrobial efficacy of antibiotics and calcium hydroxide against enterococcus faecalis biofilm in dentine

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