Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens

Bacteriocin or Bacteriocin like inhibitory substances (BLIS) is a protein antibiotic that has a relatively narrow spectrum of killing activity. It could potentially serve as a natural alternative to antibiotics in reducing the development of multi-drug resistant bacteria. Antimicrobial activity of t...

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Main Authors:	Azizah Ahmad Fauzi, Zaleha Shafiei, Badiah Baharin, Nurulhuda Mohd
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2013
Online Access:	http://journalarticle.ukm.my/5699/ http://journalarticle.ukm.my/5699/ http://journalarticle.ukm.my/5699/1/04%2520Azizah%2520Ahmad.pdf

id	ukm-5699
recordtype	eprints
spelling	ukm-56992016-12-14T06:39:14Z http://journalarticle.ukm.my/5699/ Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens Azizah Ahmad Fauzi, Zaleha Shafiei, Badiah Baharin, Nurulhuda Mohd, Bacteriocin or Bacteriocin like inhibitory substances (BLIS) is a protein antibiotic that has a relatively narrow spectrum of killing activity. It could potentially serve as a natural alternative to antibiotics in reducing the development of multi-drug resistant bacteria. Antimicrobial activity of the strains of Lactobacillus sp. isolated from healthy subjects (test strains) against Aggregatibacter actinomycetemcomitans and other periodontal pathogens (indicator strains) isolated from subgingival plaques of aggressive periodontitis patients were determined by using deferred antagonism test and agar-well diffusion method. Strains of Lactobacillus sp., Aggregatibacter actinomycetemcomitans and black pigmented bacteria were selectively isolated from TJA, TSBV and TSBA agars, respectively. Mean diameter zone of inhibition of at least 10 mm was considered as positive results for both methods. Out of 25 strains of Lactobacillus sp. screened, only eight test strains of Lactobacillus sp. showed the specific antimicrobial activity against certain strains of indicator periodontal pathogens during deferred antagonism test. However, out of eight potential strains, only three strains, which were Lactobacillus sp. strain S, Lactobacillus sp. strain V and Lactobacillus sp. strain W consistently showed positive inhibitory activity against black pigmented bacteria by deferred antagonism test and agar-well diffusion method. Therefore, these three strains should be considered as potential BLIS producer strains for further study Universiti Kebangsaan Malaysia 2013-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/5699/1/04%2520Azizah%2520Ahmad.pdf Azizah Ahmad Fauzi, and Zaleha Shafiei, and Badiah Baharin, and Nurulhuda Mohd, (2013) Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens. Sains Malaysiana, 42 (1). pp. 19-24. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Bacteriocin or Bacteriocin like inhibitory substances (BLIS) is a protein antibiotic that has a relatively narrow spectrum of killing activity. It could potentially serve as a natural alternative to antibiotics in reducing the development of multi-drug resistant bacteria. Antimicrobial activity of the strains of Lactobacillus sp. isolated from healthy subjects (test strains) against Aggregatibacter actinomycetemcomitans and other periodontal pathogens (indicator strains) isolated from subgingival plaques of aggressive periodontitis patients were determined by using deferred antagonism test and agar-well diffusion method. Strains of Lactobacillus sp., Aggregatibacter actinomycetemcomitans and black pigmented bacteria were selectively isolated from TJA, TSBV and TSBA agars, respectively. Mean diameter zone of inhibition of at least 10 mm was considered as positive results for both methods. Out of 25 strains of Lactobacillus sp. screened, only eight test strains of Lactobacillus sp. showed the specific antimicrobial activity against certain strains of indicator periodontal pathogens during deferred antagonism test. However, out of eight potential strains, only three strains, which were Lactobacillus sp. strain S, Lactobacillus sp. strain V and Lactobacillus sp. strain W consistently showed positive inhibitory activity against black pigmented bacteria by deferred antagonism test and agar-well diffusion method. Therefore, these three strains should be considered as potential BLIS producer strains for further study
format	Article
author	Azizah Ahmad Fauzi, Zaleha Shafiei, Badiah Baharin, Nurulhuda Mohd,
spellingShingle	Azizah Ahmad Fauzi, Zaleha Shafiei, Badiah Baharin, Nurulhuda Mohd, Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
author_facet	Azizah Ahmad Fauzi, Zaleha Shafiei, Badiah Baharin, Nurulhuda Mohd,
author_sort	Azizah Ahmad Fauzi,
title	Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
title_short	Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
title_full	Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
title_fullStr	Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
title_full_unstemmed	Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
title_sort	isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
publisher	Universiti Kebangsaan Malaysia
publishDate	2013
url	http://journalarticle.ukm.my/5699/ http://journalarticle.ukm.my/5699/ http://journalarticle.ukm.my/5699/1/04%2520Azizah%2520Ahmad.pdf
first_indexed	2023-09-18T19:44:52Z
last_indexed	2023-09-18T19:44:52Z
_version_	1777405833015984128

Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens

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