Limit of DNA Barcode in delineating penaeus monodon and in its developing stages

Mysis, post larvae and adult specimens of Penaeus monodon were sequenced for Cyctochrome C Oxidase subunit I (COI) gene (DNA barcode) to check the efficiency of DNA barcode in delineating species irrespective of its different life stages. COI gene sequences of different life stages of P. monodon wer...

Full description

Bibliographic Details
Main Authors:	Prasanna Kumar, C, Akbar John, B, Ajmal Khan, S, Lyla, P.S, Jalal, K.C.A
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2012
Online Access:	http://journalarticle.ukm.my/5669/ http://journalarticle.ukm.my/5669/ http://journalarticle.ukm.my/5669/1/05%2520C.%2520Prasanna%2520Kumar.pdf

id	ukm-5669
recordtype	eprints
spelling	ukm-56692016-12-14T06:39:08Z http://journalarticle.ukm.my/5669/ Limit of DNA Barcode in delineating penaeus monodon and in its developing stages Prasanna Kumar, C Akbar John, B Ajmal Khan, S Lyla, P.S Jalal, K.C.A Mysis, post larvae and adult specimens of Penaeus monodon were sequenced for Cyctochrome C Oxidase subunit I (COI) gene (DNA barcode) to check the efficiency of DNA barcode in delineating species irrespective of its different life stages. COI gene sequences of different life stages of P. monodon were subjected to pair-wise distance analysis, nucleotide sequence diversity estimation, phylogenetic and BOLD analysis. The pair-wise distance estimation revealed that intra-specific variations within the barcode sequences of P. monodon were low when compared with other species of genus Penaeus spp. The average pair-wise distance within the sequences of different stages of P. monodon was two times lesser than the pair-wise distance of the out-group used. The nucleotide diversity within the barcodes were low (π = 0.324). The constructed phylogram clearly showed that the DNA barcoding using partial COI gene would provide an accurate delineation of species irrespective of their different life stages. The BOLD analysis accurately identified all sequences as belonging to P. monodon. Universiti Kebangsaan Malaysia 2012-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/5669/1/05%2520C.%2520Prasanna%2520Kumar.pdf Prasanna Kumar, C and Akbar John, B and Ajmal Khan, S and Lyla, P.S and Jalal, K.C.A (2012) Limit of DNA Barcode in delineating penaeus monodon and in its developing stages. Sains Malaysiana, 41 (12). pp. 1527-1533. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Mysis, post larvae and adult specimens of Penaeus monodon were sequenced for Cyctochrome C Oxidase subunit I (COI) gene (DNA barcode) to check the efficiency of DNA barcode in delineating species irrespective of its different life stages. COI gene sequences of different life stages of P. monodon were subjected to pair-wise distance analysis, nucleotide sequence diversity estimation, phylogenetic and BOLD analysis. The pair-wise distance estimation revealed that intra-specific variations within the barcode sequences of P. monodon were low when compared with other species of genus Penaeus spp. The average pair-wise distance within the sequences of different stages of P. monodon was two times lesser than the pair-wise distance of the out-group used. The nucleotide diversity within the barcodes were low (π = 0.324). The constructed phylogram clearly showed that the DNA barcoding using partial COI gene would provide an accurate delineation of species irrespective of their different life stages. The BOLD analysis accurately identified all sequences as belonging to P. monodon.
format	Article
author	Prasanna Kumar, C Akbar John, B Ajmal Khan, S Lyla, P.S Jalal, K.C.A
spellingShingle	Prasanna Kumar, C Akbar John, B Ajmal Khan, S Lyla, P.S Jalal, K.C.A Limit of DNA Barcode in delineating penaeus monodon and in its developing stages
author_facet	Prasanna Kumar, C Akbar John, B Ajmal Khan, S Lyla, P.S Jalal, K.C.A
author_sort	Prasanna Kumar, C
title	Limit of DNA Barcode in delineating penaeus monodon and in its developing stages
title_short	Limit of DNA Barcode in delineating penaeus monodon and in its developing stages
title_full	Limit of DNA Barcode in delineating penaeus monodon and in its developing stages
title_fullStr	Limit of DNA Barcode in delineating penaeus monodon and in its developing stages
title_full_unstemmed	Limit of DNA Barcode in delineating penaeus monodon and in its developing stages
title_sort	limit of dna barcode in delineating penaeus monodon and in its developing stages
publisher	Universiti Kebangsaan Malaysia
publishDate	2012
url	http://journalarticle.ukm.my/5669/ http://journalarticle.ukm.my/5669/ http://journalarticle.ukm.my/5669/1/05%2520C.%2520Prasanna%2520Kumar.pdf
first_indexed	2023-09-18T19:44:48Z
last_indexed	2023-09-18T19:44:48Z
_version_	1777405828642373632

Limit of DNA Barcode in delineating penaeus monodon and in its developing stages

Similar Items