In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine

Acanthamoeba keratitis is a serious infection of the eye which can result in permanent visual impairment. Therefore this study was performed to evaluate the effectiveness of antimicrobial agents on three Acanthamoeba clinical isolates (HS 6, HKL 95 and HTH 73). Antimicrobial agents used in this stud...

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Main Authors:	Noradilah Samseh Abdullah, Mohamed Kamel Abd Ghani, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2012
Online Access:	http://journalarticle.ukm.my/4469/ http://journalarticle.ukm.my/4469/ http://journalarticle.ukm.my/4469/1/07%2520Noradilah.pdf

id	ukm-4469
recordtype	eprints
spelling	ukm-44692016-12-14T06:36:06Z http://journalarticle.ukm.my/4469/ In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine Noradilah Samseh Abdullah, Mohamed Kamel Abd Ghani, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad, Acanthamoeba keratitis is a serious infection of the eye which can result in permanent visual impairment. Therefore this study was performed to evaluate the effectiveness of antimicrobial agents on three Acanthamoeba clinical isolates (HS 6, HKL 95 and HTH 73). Antimicrobial agents used in this study were polyhexamethylene biguanide (PHMB) and chlorhexidine and both were serially diluted. Cyst suspensions from all three strains were tested against the antimicrobial agents, respectively. After 48 h of incubation at 37°C, the suspension was filtered and the filter membrane was placed onto non-nutrient agar plate lawned with heat-killed Escherichia coli. The plates were examined daily under the inverted microscope until day 14 but were negative for Acanthamoeba trophozoites. The presence of trophozoites indicated ineffectiveness of the antimicrobial agents. Both antimicrobial agents used were found to be effective against Acanthamoeba cysts for all the strains tested. PHMB gave minimum cysticidal concentration (MCC) mean value of 4.232 μg/mL and chlorhexidine showed MCC mean value of 3.906 μg/mL. So, from this study, it can be concluded that PHMB and chlorhexidine were effective in killing the tested Acanthamoeba cysts Universiti Kebangsaan Malaysia 2012-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/4469/1/07%2520Noradilah.pdf Noradilah Samseh Abdullah, and Mohamed Kamel Abd Ghani, and Anisah Nordin, and Yusof Suboh, and Noraina Ab Rahim, and Norazah Ahmad, (2012) In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine. Sains Malaysiana, 41 (5). pp. 569-572. ISSN 0126-6039 http://www.ukm.my/jsm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Acanthamoeba keratitis is a serious infection of the eye which can result in permanent visual impairment. Therefore this study was performed to evaluate the effectiveness of antimicrobial agents on three Acanthamoeba clinical isolates (HS 6, HKL 95 and HTH 73). Antimicrobial agents used in this study were polyhexamethylene biguanide (PHMB) and chlorhexidine and both were serially diluted. Cyst suspensions from all three strains were tested against the antimicrobial agents, respectively. After 48 h of incubation at 37°C, the suspension was filtered and the filter membrane was placed onto non-nutrient agar plate lawned with heat-killed Escherichia coli. The plates were examined daily under the inverted microscope until day 14 but were negative for Acanthamoeba trophozoites. The presence of trophozoites indicated ineffectiveness of the antimicrobial agents. Both antimicrobial agents used were found to be effective against Acanthamoeba cysts for all the strains tested. PHMB gave minimum cysticidal concentration (MCC) mean value of 4.232 μg/mL and chlorhexidine showed MCC mean value of 3.906 μg/mL. So, from this study, it can be concluded that PHMB and chlorhexidine were effective in killing the tested Acanthamoeba cysts
format	Article
author	Noradilah Samseh Abdullah, Mohamed Kamel Abd Ghani, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad,
spellingShingle	Noradilah Samseh Abdullah, Mohamed Kamel Abd Ghani, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad, In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine
author_facet	Noradilah Samseh Abdullah, Mohamed Kamel Abd Ghani, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad,
author_sort	Noradilah Samseh Abdullah,
title	In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine
title_short	In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine
title_full	In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine
title_fullStr	In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine
title_full_unstemmed	In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine
title_sort	in vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (phmb) and chlorhexidine
publisher	Universiti Kebangsaan Malaysia
publishDate	2012
url	http://journalarticle.ukm.my/4469/ http://journalarticle.ukm.my/4469/ http://journalarticle.ukm.my/4469/1/07%2520Noradilah.pdf
first_indexed	2023-09-18T19:41:38Z
last_indexed	2023-09-18T19:41:38Z
_version_	1777405629059563520

In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine

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