Development of resistance in candida tropicalis : an in vitro study

A study was conducted to determine the development of resistance in Candida tropicalis against antifungal agents after long-term exposure to low drug concentrations. Five clinical isolates of Candida tropicalis were selected for this study. These isolates were found to have already shown resistance...

Full description

Bibliographic Details
Main Authors:	J. Santhanam, Fairuz A, Ooi KF, Muhamad Husni A.
Format:	Article
Language:	English
Published:	Department Of Community Health, Faculty of Medicine, Universiti Kebangsaan Malaysia 2002
Online Access:	http://journalarticle.ukm.my/4417/ http://journalarticle.ukm.my/4417/ http://journalarticle.ukm.my/4417/1/1.pdf

id	ukm-4417
recordtype	eprints
spelling	ukm-44172016-12-14T06:35:58Z http://journalarticle.ukm.my/4417/ Development of resistance in candida tropicalis : an in vitro study J. Santhanam, Fairuz A, Ooi KF, Muhamad Husni A., A study was conducted to determine the development of resistance in Candida tropicalis against antifungal agents after long-term exposure to low drug concentrations. Five clinical isolates of Candida tropicalis were selected for this study. These isolates were found to have already shown resistance to either amphotericin B or to fluconazole. Two isolates that were sensitive to fluconazole were exposed to 0.5 ?g/ml of fluconazole in a broth culture (Sabouraud dextrose broth) with frequent subculturing for up to 14 weeks. Three isolates that were sensitive to amphotericin B were exposed to 0.25 – 0.5 ?g/ml of amphotericin B in the same way. At different time intervals, the MIC values of the isolates were determined by the broth microdilution test as proposed by the National Committee for Clinical Laboratory Standards (NCCLS). Our results showed a 4-fold increase in the MIC values after 10 weeks of exposure to the antifungal agent, which continued to rise until the end of the experiment. Control isolates not exposed to antifungal agents showed no increase in their MIC values. Protein profiles of all isolates (using sodium dodecyl sulphate polyacrylamide gel electrophoresis) were obtained before and after exposure to antifungal agent. A change in the protein profile (new fractions or increased concentration) was observed for all isolates. In conclusion, induction of resistance was observed in Candida tropicalis isolates exposed to antifungal agents and this occurrence correlates with a change in the protein profile. Department Of Community Health, Faculty of Medicine, Universiti Kebangsaan Malaysia 2002 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/4417/1/1.pdf J. Santhanam, and Fairuz A, and Ooi KF, and Muhamad Husni A., (2002) Development of resistance in candida tropicalis : an in vitro study. Jurnal Kesihatan Masyarakat, 8 (S). pp. 50-53. ISSN 1675-1663 http://www.communityhealthjournal.org/detailarticle.asp?id=321&issue=Vol8(S):2002
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	A study was conducted to determine the development of resistance in Candida tropicalis against antifungal agents after long-term exposure to low drug concentrations. Five clinical isolates of Candida tropicalis were selected for this study. These isolates were found to have already shown resistance to either amphotericin B or to fluconazole. Two isolates that were sensitive to fluconazole were exposed to 0.5 ?g/ml of fluconazole in a broth culture (Sabouraud dextrose broth) with frequent subculturing for up to 14 weeks. Three isolates that were sensitive to amphotericin B were exposed to 0.25 – 0.5 ?g/ml of amphotericin B in the same way. At different time intervals, the MIC values of the isolates were determined by the broth microdilution test as proposed by the National Committee for Clinical Laboratory Standards (NCCLS). Our results showed a 4-fold increase in the MIC values after 10 weeks of exposure to the antifungal agent, which continued to rise until the end of the experiment. Control isolates not exposed to antifungal agents showed no increase in their MIC values. Protein profiles of all isolates (using sodium dodecyl sulphate polyacrylamide gel electrophoresis) were obtained before and after exposure to antifungal agent. A change in the protein profile (new fractions or increased concentration) was observed for all isolates. In conclusion, induction of resistance was observed in Candida tropicalis isolates exposed to antifungal agents and this occurrence correlates with a change in the protein profile.
format	Article
author	J. Santhanam, Fairuz A, Ooi KF, Muhamad Husni A.,
spellingShingle	J. Santhanam, Fairuz A, Ooi KF, Muhamad Husni A., Development of resistance in candida tropicalis : an in vitro study
author_facet	J. Santhanam, Fairuz A, Ooi KF, Muhamad Husni A.,
author_sort	J. Santhanam,
title	Development of resistance in candida tropicalis : an in vitro study
title_short	Development of resistance in candida tropicalis : an in vitro study
title_full	Development of resistance in candida tropicalis : an in vitro study
title_fullStr	Development of resistance in candida tropicalis : an in vitro study
title_full_unstemmed	Development of resistance in candida tropicalis : an in vitro study
title_sort	development of resistance in candida tropicalis : an in vitro study
publisher	Department Of Community Health, Faculty of Medicine, Universiti Kebangsaan Malaysia
publishDate	2002
url	http://journalarticle.ukm.my/4417/ http://journalarticle.ukm.my/4417/ http://journalarticle.ukm.my/4417/1/1.pdf
first_indexed	2023-09-18T19:41:28Z
last_indexed	2023-09-18T19:41:28Z
_version_	1777405618892570624

Development of resistance in candida tropicalis : an in vitro study

Similar Items