Inoculum development for lipid and gamma linoenic asid production by Cunninghamella bainieri2A1)

Inoculum development for lipid and gamma linoenic asid production by Cunninghamella bainieri2A1)

Inoculum for a local isolate of oleaginus fungi, Cunninghamella bainieri 2A1 was developed by establishing the inoculum types, age and size for growth, lipid and gamma linolenic acid (GLA) production. Cultivation was carried out in 500 mL shake flask containing 200 mL of nitrogen limiting medium at...

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Main Authors: Farhila Muhid, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff, Othman Omar, Aidil Abdul Hamid
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2012
Online Access:http://journalarticle.ukm.my/3932/
http://journalarticle.ukm.my/3932/
http://journalarticle.ukm.my/3932/1/07%2520Farhila.pdf
id ukm-3932
recordtype eprints
spelling ukm-39322016-12-14T06:35:21Z http://journalarticle.ukm.my/3932/ Inoculum development for lipid and gamma linoenic asid production by Cunninghamella bainieri2A1) Farhila Muhid, Abdul Jalil Abdul Kader, Wan Mohtar Wan Yusoff, Othman Omar, Aidil Abdul Hamid, Inoculum for a local isolate of oleaginus fungi, Cunninghamella bainieri 2A1 was developed by establishing the inoculum types, age and size for growth, lipid and gamma linolenic acid (GLA) production. Cultivation was carried out in 500 mL shake flask containing 200 mL of nitrogen limiting medium at 30°C and 250 rpm agitation. Direct inoculation of spores into the production cultures gave higher productivity of lipid production 0.71 (g/L/day) than using 24 and 48 vegetative cells cultures which gave only 0.51 and 0.45 (g/L/day), respectively. Besides, production of GLA (5.3 × 10-2 g/g lipid less biomass) in the cultures which directly inoculated with spores (1 × 105 spores/mL) was 23% higher than those produced in the cultures started with vegetative cells. Spore concentration of 1 × 103 spores/mL produced pelletal growth in size of 1.04 mm which contain 40% (g/g biomass) lipid and 8.34 × 10-2 (g/g lipid less biomass). Universiti Kebangsaan Malaysia 2012-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/3932/1/07%2520Farhila.pdf Farhila Muhid, and Abdul Jalil Abdul Kader, and Wan Mohtar Wan Yusoff, and Othman Omar, and Aidil Abdul Hamid, (2012) Inoculum development for lipid and gamma linoenic asid production by Cunninghamella bainieri2A1). Sains Malaysiana, 41 (4). pp. 431-437. ISSN 0126-6039 http://www.ukm.my/jsm
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institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description Inoculum for a local isolate of oleaginus fungi, Cunninghamella bainieri 2A1 was developed by establishing the inoculum types, age and size for growth, lipid and gamma linolenic acid (GLA) production. Cultivation was carried out in 500 mL shake flask containing 200 mL of nitrogen limiting medium at 30°C and 250 rpm agitation. Direct inoculation of spores into the production cultures gave higher productivity of lipid production 0.71 (g/L/day) than using 24 and 48 vegetative cells cultures which gave only 0.51 and 0.45 (g/L/day), respectively. Besides, production of GLA (5.3 × 10-2 g/g lipid less biomass) in the cultures which directly inoculated with spores (1 × 105 spores/mL) was 23% higher than those produced in the cultures started with vegetative cells. Spore concentration of 1 × 103 spores/mL produced pelletal growth in size of 1.04 mm which contain 40% (g/g biomass) lipid and 8.34 × 10-2 (g/g lipid less biomass).
format Article
author Farhila Muhid,
Abdul Jalil Abdul Kader,
Wan Mohtar Wan Yusoff,
Othman Omar,
Aidil Abdul Hamid,
spellingShingle Farhila Muhid,
Abdul Jalil Abdul Kader,
Wan Mohtar Wan Yusoff,
Othman Omar,
Aidil Abdul Hamid,
Inoculum development for lipid and gamma linoenic asid production by Cunninghamella bainieri2A1)
author_facet Farhila Muhid,
Abdul Jalil Abdul Kader,
Wan Mohtar Wan Yusoff,
Othman Omar,
Aidil Abdul Hamid,
author_sort Farhila Muhid,
title Inoculum development for lipid and gamma linoenic asid production by Cunninghamella bainieri2A1)
title_short Inoculum development for lipid and gamma linoenic asid production by Cunninghamella bainieri2A1)
title_full Inoculum development for lipid and gamma linoenic asid production by Cunninghamella bainieri2A1)
title_fullStr Inoculum development for lipid and gamma linoenic asid production by Cunninghamella bainieri2A1)
title_full_unstemmed Inoculum development for lipid and gamma linoenic asid production by Cunninghamella bainieri2A1)
title_sort inoculum development for lipid and gamma linoenic asid production by cunninghamella bainieri2a1)
publisher Universiti Kebangsaan Malaysia
publishDate 2012
url http://journalarticle.ukm.my/3932/
http://journalarticle.ukm.my/3932/
http://journalarticle.ukm.my/3932/1/07%2520Farhila.pdf
first_indexed 2023-09-18T19:40:09Z
last_indexed 2023-09-18T19:40:09Z
_version_ 1777405535860031488

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