Challenges in culturing macaca fascicularisBone marrow stem cells

Challenges in culturing macaca fascicularisBone marrow stem cells

Culturing Macaca fascicularis bone marrow stem cells in fetal bovine serum (FBS) resulted in low proliferation and long period of incubation. Therefore, its potential uses are exhausted. Here we report the establishment of culturing the Macaca fascicularis bone marrow stem cells using the FBS in com...

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Main Authors: Sharen, S@Sharen Aini, Masfueh, R, Badiah, B, Ng, M.H, Shamsul, B.S, Chua, K.H, Low, C.K, Mohd Nazimi, A.J, Rifqah, N, Norziha, Y, Shariffah Shuriana, A.S, Ruszymah, B.H.I, Siar, C, Ramli, R
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2012
Online Access:http://journalarticle.ukm.my/3930/
http://journalarticle.ukm.my/3930/
http://journalarticle.ukm.my/3930/1/05%2520Sharen.pdf
id ukm-3930
recordtype eprints
spelling ukm-39302016-12-14T06:35:20Z http://journalarticle.ukm.my/3930/ Challenges in culturing macaca fascicularisBone marrow stem cells Sharen, S@Sharen Aini Masfueh, R Badiah, B Ng, M.H Shamsul, B.S Chua, K.H Low, C.K Mohd Nazimi, A.J Rifqah, N Norziha, Y Shariffah Shuriana, A.S Ruszymah, B.H.I Siar, C Ramli, R Culturing Macaca fascicularis bone marrow stem cells in fetal bovine serum (FBS) resulted in low proliferation and long period of incubation. Therefore, its potential uses are exhausted. Here we report the establishment of culturing the Macaca fascicularis bone marrow stem cells using the FBS in combination with autologous serum. Five percent autologous serum was added to the Minimum Essential Medium (MEM) alpha medium and 10% FBS while 0.2 mM acid ascorbic 2-phosphate, 10 mM β-glycerolphosphate, 10-8 molar dexamethasone were used for osteogenic induction. Following this combination, our results showed higher growth kinetic i.e. 1.41% growth rate higher compared to only 0.46% growth rates of the cells using FBS alone and shorter population doubling time (4 to 7 days) compared to the culture without the combination of FBS and autologous serum (30 days). Thus, the combination of the FBS and autologous serum permits fast cell growth and tissue construction. Universiti Kebangsaan Malaysia 2012-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/3930/1/05%2520Sharen.pdf Sharen, S@Sharen Aini and Masfueh, R and Badiah, B and Ng, M.H and Shamsul, B.S and Chua, K.H and Low, C.K and Mohd Nazimi, A.J and Rifqah, N and Norziha, Y and Shariffah Shuriana, A.S and Ruszymah, B.H.I and Siar, C and Ramli, R (2012) Challenges in culturing macaca fascicularisBone marrow stem cells. Sains Malaysiana, 41 (4). pp. 419-422. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description Culturing Macaca fascicularis bone marrow stem cells in fetal bovine serum (FBS) resulted in low proliferation and long period of incubation. Therefore, its potential uses are exhausted. Here we report the establishment of culturing the Macaca fascicularis bone marrow stem cells using the FBS in combination with autologous serum. Five percent autologous serum was added to the Minimum Essential Medium (MEM) alpha medium and 10% FBS while 0.2 mM acid ascorbic 2-phosphate, 10 mM β-glycerolphosphate, 10-8 molar dexamethasone were used for osteogenic induction. Following this combination, our results showed higher growth kinetic i.e. 1.41% growth rate higher compared to only 0.46% growth rates of the cells using FBS alone and shorter population doubling time (4 to 7 days) compared to the culture without the combination of FBS and autologous serum (30 days). Thus, the combination of the FBS and autologous serum permits fast cell growth and tissue construction.
format Article
author Sharen, S@Sharen Aini
Masfueh, R
Badiah, B
Ng, M.H
Shamsul, B.S
Chua, K.H
Low, C.K
Mohd Nazimi, A.J
Rifqah, N
Norziha, Y
Shariffah Shuriana, A.S
Ruszymah, B.H.I
Siar, C
Ramli, R
spellingShingle Sharen, S@Sharen Aini
Masfueh, R
Badiah, B
Ng, M.H
Shamsul, B.S
Chua, K.H
Low, C.K
Mohd Nazimi, A.J
Rifqah, N
Norziha, Y
Shariffah Shuriana, A.S
Ruszymah, B.H.I
Siar, C
Ramli, R
Challenges in culturing macaca fascicularisBone marrow stem cells
author_facet Sharen, S@Sharen Aini
Masfueh, R
Badiah, B
Ng, M.H
Shamsul, B.S
Chua, K.H
Low, C.K
Mohd Nazimi, A.J
Rifqah, N
Norziha, Y
Shariffah Shuriana, A.S
Ruszymah, B.H.I
Siar, C
Ramli, R
author_sort Sharen, S@Sharen Aini
title Challenges in culturing macaca fascicularisBone marrow stem cells
title_short Challenges in culturing macaca fascicularisBone marrow stem cells
title_full Challenges in culturing macaca fascicularisBone marrow stem cells
title_fullStr Challenges in culturing macaca fascicularisBone marrow stem cells
title_full_unstemmed Challenges in culturing macaca fascicularisBone marrow stem cells
title_sort challenges in culturing macaca fascicularisbone marrow stem cells
publisher Universiti Kebangsaan Malaysia
publishDate 2012
url http://journalarticle.ukm.my/3930/
http://journalarticle.ukm.my/3930/
http://journalarticle.ukm.my/3930/1/05%2520Sharen.pdf
first_indexed 2023-09-18T19:40:09Z
last_indexed 2023-09-18T19:40:09Z
_version_ 1777405535584256000

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