Sources of possible inoculum for horizontal transmission of nosema bombycis in diamondback moth, plutella xylostella (L.)

A study to identify possible sources of inoculum for horizontal transmission (within generation) of Nosema bombycis spores in diamondback moth (DBM), Plutella xylostella (L.), larvae were conducted. The body excretions (frass, silk, regurgitate and exuviae) of DBM larvae, and meconium of newly emerg...

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Main Authors:	Idris, A. B, Grafius, E.
Format:	Article
Published:	Universiti Kebangsaan Malaysia 1999
Online Access:	http://journalarticle.ukm.my/3765/ http://journalarticle.ukm.my/3765/

id	ukm-3765
recordtype	eprints
spelling	ukm-37652012-05-02T09:45:48Z http://journalarticle.ukm.my/3765/ Sources of possible inoculum for horizontal transmission of nosema bombycis in diamondback moth, plutella xylostella (L.) Idris, A. B Grafius, E. A study to identify possible sources of inoculum for horizontal transmission (within generation) of Nosema bombycis spores in diamondback moth (DBM), Plutella xylostella (L.), larvae were conducted. The body excretions (frass, silk, regurgitate and exuviae) of DBM larvae, and meconium of newly emerged DBM adults reared from Nosema-infected larvae were examined for N. bombycis spores. The presence and relative abundance of spores were observed microscopically. The effect of Nosema-contaminated Diadegma insulare (Cresson) on parasitism of DBM larvae was also investigated. Percentage infection and mortality of DBM larvae were used as evidence for the parasitoid's role in spore transmission among neonate DBM larvae. Results showed that all body excretions and the exuviae had N. bombycis spores or can be sources of Nosema spores. This suggests that these excretions are sources of N. bombycis spores for horizontal transmission. The spores were relatively more abundant in the late DBM instars than in the early instars. Percent infection and percentage mortality of DBM larvae parasitised by the Nosema-infected D. insulare was significantly higher when parasitism occurred in the early instars than in the late instars. Methods of avoiding N. bombycis infection in laboratory rearing of DBM are suggested. The importance of D. insulare as a major source for Nosema transmission and its possible diminishing role as biological control agent of DBM in the field are also discussed. Universiti Kebangsaan Malaysia 1999 Article PeerReviewed Idris, A. B and Grafius, E. (1999) Sources of possible inoculum for horizontal transmission of nosema bombycis in diamondback moth, plutella xylostella (L.). Sains Malaysiana, 28 . pp. 39-47. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol28_1999/vol28_99page39-47.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
description	A study to identify possible sources of inoculum for horizontal transmission (within generation) of Nosema bombycis spores in diamondback moth (DBM), Plutella xylostella (L.), larvae were conducted. The body excretions (frass, silk, regurgitate and exuviae) of DBM larvae, and meconium of newly emerged DBM adults reared from Nosema-infected larvae were examined for N. bombycis spores. The presence and relative abundance of spores were observed microscopically. The effect of Nosema-contaminated Diadegma insulare (Cresson) on parasitism of DBM larvae was also investigated. Percentage infection and mortality of DBM larvae were used as evidence for the parasitoid's role in spore transmission among neonate DBM larvae. Results showed that all body excretions and the exuviae had N. bombycis spores or can be sources of Nosema spores. This suggests that these excretions are sources of N. bombycis spores for horizontal transmission. The spores were relatively more abundant in the late DBM instars than in the early instars. Percent infection and percentage mortality of DBM larvae parasitised by the Nosema-infected D. insulare was significantly higher when parasitism occurred in the early instars than in the late instars. Methods of avoiding N. bombycis infection in laboratory rearing of DBM are suggested. The importance of D. insulare as a major source for Nosema transmission and its possible diminishing role as biological control agent of DBM in the field are also discussed.
format	Article
author	Idris, A. B Grafius, E.
spellingShingle	Idris, A. B Grafius, E. Sources of possible inoculum for horizontal transmission of nosema bombycis in diamondback moth, plutella xylostella (L.)
author_facet	Idris, A. B Grafius, E.
author_sort	Idris, A. B
title	Sources of possible inoculum for horizontal transmission of nosema bombycis in diamondback moth, plutella xylostella (L.)
title_short	Sources of possible inoculum for horizontal transmission of nosema bombycis in diamondback moth, plutella xylostella (L.)
title_full	Sources of possible inoculum for horizontal transmission of nosema bombycis in diamondback moth, plutella xylostella (L.)
title_fullStr	Sources of possible inoculum for horizontal transmission of nosema bombycis in diamondback moth, plutella xylostella (L.)
title_full_unstemmed	Sources of possible inoculum for horizontal transmission of nosema bombycis in diamondback moth, plutella xylostella (L.)
title_sort	sources of possible inoculum for horizontal transmission of nosema bombycis in diamondback moth, plutella xylostella (l.)
publisher	Universiti Kebangsaan Malaysia
publishDate	1999
url	http://journalarticle.ukm.my/3765/ http://journalarticle.ukm.my/3765/
first_indexed	2023-09-18T19:39:41Z
last_indexed	2023-09-18T19:39:41Z
_version_	1777405506856419328

Sources of possible inoculum for horizontal transmission of nosema bombycis in diamondback moth, plutella xylostella (L.)

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