Immunofluorescence (IF) pattern, autoantigens of anti-neutrophil cytoplasmic antibodies (ANCA) and their clinical associations

and monocytes’ lysosomes. In general, c-ANCA is strongly associated with vasculitic disorders mainly in ANCA-associated systemic vasculitis (AASV). p-ANCA have been identified in several diseases such as primary (AASV) and secondary vasculitis such as collagen vascular diseases, rheumatoid arthritis...

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Main Authors:	Masita Arip, Marlyn Mohammad, Salbiah Nawi, Shahnaz Murad
Format:	Article
Language:	English
Published:	Penerbit UKM 2010
Online Access:	http://journalarticle.ukm.my/3671/ http://journalarticle.ukm.my/3671/ http://journalarticle.ukm.my/3671/1/Immunofluorescence_%28IF%29_Pattern%2C_Autoantigens.pdf

id	ukm-3671
recordtype	eprints
spelling	ukm-36712016-12-14T06:35:05Z http://journalarticle.ukm.my/3671/ Immunofluorescence (IF) pattern, autoantigens of anti-neutrophil cytoplasmic antibodies (ANCA) and their clinical associations Masita Arip, Marlyn Mohammad, Salbiah Nawi, Shahnaz Murad, and monocytes’ lysosomes. In general, c-ANCA is strongly associated with vasculitic disorders mainly in ANCA-associated systemic vasculitis (AASV). p-ANCA have been identified in several diseases such as primary (AASV) and secondary vasculitis such as collagen vascular diseases, rheumatoid arthritis and inflammatory bowel diseases given the term ‘ANCA-associated disease.’ The objective of this study was to determine the rate of ANCA positivity by indirect immunofluorescent (IF) and enzyme linked immunosorbent assay (ELISA) and its association with AASV and ANCA associated diseases. Serum from patients with history suspicion of systemic vasculitis were tested for ANCA by IF. Those samples positive for ANCA by IF were further tested for antibodies against myeloperoxidase (MPO) and proteinase 3 (PR3) using the ELISA. Clinical data from medical records were obtained and analyzed. Of 468 samples, a total of 110 were positive for ANCA by IF. IF results showed a p-ANCA pattern in 96 patients and c-ANCA in 14. Of 110 IF positive ANCA, 45 patients were positive by ELISA. Seventeen were positive for MPO-ANCA, 9 were PR3-ANCA positive and 19 were both MPO and PR3 positive. Only 2 patients were classified AASV ie Wegener granulomatosis and the other with microscopic polyangiitis. The remaining patients (n = 108) may be classified as ANCA associated diseases. Our study showed that p- ANCA (87.3%) was the more common ANCA pattern and 40.9% of IF positive samples were positive for PR3- and MPOANCA. Penerbit UKM 2010 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/3671/1/Immunofluorescence_%28IF%29_Pattern%2C_Autoantigens.pdf Masita Arip, and Marlyn Mohammad, and Salbiah Nawi, and Shahnaz Murad, (2010) Immunofluorescence (IF) pattern, autoantigens of anti-neutrophil cytoplasmic antibodies (ANCA) and their clinical associations. Jurnal Sains Kesihatan Malaysia, 8 (2). pp. 37-41. ISSN 1675-8161 http://www.fsk.ukm.my/jurnal/jilid%208%282%29%202010/Chap%207.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	and monocytes’ lysosomes. In general, c-ANCA is strongly associated with vasculitic disorders mainly in ANCA-associated systemic vasculitis (AASV). p-ANCA have been identified in several diseases such as primary (AASV) and secondary vasculitis such as collagen vascular diseases, rheumatoid arthritis and inflammatory bowel diseases given the term ‘ANCA-associated disease.’ The objective of this study was to determine the rate of ANCA positivity by indirect immunofluorescent (IF) and enzyme linked immunosorbent assay (ELISA) and its association with AASV and ANCA associated diseases. Serum from patients with history suspicion of systemic vasculitis were tested for ANCA by IF. Those samples positive for ANCA by IF were further tested for antibodies against myeloperoxidase (MPO) and proteinase 3 (PR3) using the ELISA. Clinical data from medical records were obtained and analyzed. Of 468 samples, a total of 110 were positive for ANCA by IF. IF results showed a p-ANCA pattern in 96 patients and c-ANCA in 14. Of 110 IF positive ANCA, 45 patients were positive by ELISA. Seventeen were positive for MPO-ANCA, 9 were PR3-ANCA positive and 19 were both MPO and PR3 positive. Only 2 patients were classified AASV ie Wegener granulomatosis and the other with microscopic polyangiitis. The remaining patients (n = 108) may be classified as ANCA associated diseases. Our study showed that p- ANCA (87.3%) was the more common ANCA pattern and 40.9% of IF positive samples were positive for PR3- and MPOANCA.
format	Article
author	Masita Arip, Marlyn Mohammad, Salbiah Nawi, Shahnaz Murad,
spellingShingle	Masita Arip, Marlyn Mohammad, Salbiah Nawi, Shahnaz Murad, Immunofluorescence (IF) pattern, autoantigens of anti-neutrophil cytoplasmic antibodies (ANCA) and their clinical associations
author_facet	Masita Arip, Marlyn Mohammad, Salbiah Nawi, Shahnaz Murad,
author_sort	Masita Arip,
title	Immunofluorescence (IF) pattern, autoantigens of anti-neutrophil cytoplasmic antibodies (ANCA) and their clinical associations
title_short	Immunofluorescence (IF) pattern, autoantigens of anti-neutrophil cytoplasmic antibodies (ANCA) and their clinical associations
title_full	Immunofluorescence (IF) pattern, autoantigens of anti-neutrophil cytoplasmic antibodies (ANCA) and their clinical associations
title_fullStr	Immunofluorescence (IF) pattern, autoantigens of anti-neutrophil cytoplasmic antibodies (ANCA) and their clinical associations
title_full_unstemmed	Immunofluorescence (IF) pattern, autoantigens of anti-neutrophil cytoplasmic antibodies (ANCA) and their clinical associations
title_sort	immunofluorescence (if) pattern, autoantigens of anti-neutrophil cytoplasmic antibodies (anca) and their clinical associations
publisher	Penerbit UKM
publishDate	2010
url	http://journalarticle.ukm.my/3671/ http://journalarticle.ukm.my/3671/ http://journalarticle.ukm.my/3671/1/Immunofluorescence_%28IF%29_Pattern%2C_Autoantigens.pdf
first_indexed	2023-09-18T19:39:25Z
last_indexed	2023-09-18T19:39:25Z
_version_	1777405490296258560

Immunofluorescence (IF) pattern, autoantigens of anti-neutrophil cytoplasmic antibodies (ANCA) and their clinical associations

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