Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures

Tocopherols and tocotrienols have been shown in previous studies to protect neurons from oxidative injuries, especially from hydrogen peroxide (H2O2) and buthionine sulfoximine (BSO) induced oxidative stress. In this study, we compared two vitamin E isomers, γ-tocotrienol (GTT) and α-tocopherol (ATF...

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Main Authors:	Then Sue-Mian, Wan Zurinah Wan NgH, Gapor Mat Top, Musalmah Mazlan
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2010
Online Access:	http://journalarticle.ukm.my/338/ http://journalarticle.ukm.my/338/ http://journalarticle.ukm.my/338/1/

id	ukm-338
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spelling	ukm-3382016-12-14T06:27:06Z http://journalarticle.ukm.my/338/ Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures Then Sue-Mian, Wan Zurinah Wan NgH, Gapor Mat Top, Musalmah Mazlan, Tocopherols and tocotrienols have been shown in previous studies to protect neurons from oxidative injuries, especially from hydrogen peroxide (H2O2) and buthionine sulfoximine (BSO) induced oxidative stress. In this study, we compared two vitamin E isomers, γ-tocotrienol (GTT) and α-tocopherol (ATF) in their neuroprotective effects against H2O2-induced apoptosis in primary rat cortical neurons and human neuroblastoma cell line SH-SY5Y. Cytotoxicity screening of H2O2, GTT and ATF was done to determine the IC50 levels. To screen for neuroprotective effects, cortical neurons and SH-SY5Y cell cultures were pre-incubated with GTT or ATF, respectively at different concentrations for 1 hour before concurrent treatment of H2O2 at IC50. Results of these treatments were compared to cells treated with H2O2 only and control cells. Cytotoxicology screening showed that IC50 of H2O2 for cortical neuron is at 50 μM while SH-SY5Y have higher IC50 of 100 μM. GTT is cytotoxic to cortical neurons at ≥50 μM and SH-SY5Y at ≥100 μM while ATF did not show any toxicity within the range of concentration tested (1-750 μM). Results from neuroprotection screening showed that GTT and ATF were able to protect both cortical neurons and SH-SY5Y from H2O2-induced oxidative stress at concetration of ≤10 μM. Cellular uptake of GTT is higher in both cortical neurons and SH-SY5Y as compared to ATF when both cortical neuron and SH-SY5Y were incubated with 10 μM GTT or ATF, respectively for 24 hour. Although primary rat cortical neurons and human neuroblastoma SH-SY5Y were different culture system, the effects of GTT and ATF are similar in both H2O2 –induced culture which strongly suggest that both GTT and ATF act as free radical scavenger to exert their neuroprotective effects. Universiti Kebangsaan Malaysia 2010-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/338/1/ Then Sue-Mian, and Wan Zurinah Wan NgH, and Gapor Mat Top, and Musalmah Mazlan, (2010) Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures. Sains Malaysiana, 39 (1). pp. 145-156. ISSN 0126-6039 http://www.ukm.my/~jsm/contents.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Tocopherols and tocotrienols have been shown in previous studies to protect neurons from oxidative injuries, especially from hydrogen peroxide (H2O2) and buthionine sulfoximine (BSO) induced oxidative stress. In this study, we compared two vitamin E isomers, γ-tocotrienol (GTT) and α-tocopherol (ATF) in their neuroprotective effects against H2O2-induced apoptosis in primary rat cortical neurons and human neuroblastoma cell line SH-SY5Y. Cytotoxicity screening of H2O2, GTT and ATF was done to determine the IC50 levels. To screen for neuroprotective effects, cortical neurons and SH-SY5Y cell cultures were pre-incubated with GTT or ATF, respectively at different concentrations for 1 hour before concurrent treatment of H2O2 at IC50. Results of these treatments were compared to cells treated with H2O2 only and control cells. Cytotoxicology screening showed that IC50 of H2O2 for cortical neuron is at 50 μM while SH-SY5Y have higher IC50 of 100 μM. GTT is cytotoxic to cortical neurons at ≥50 μM and SH-SY5Y at ≥100 μM while ATF did not show any toxicity within the range of concentration tested (1-750 μM). Results from neuroprotection screening showed that GTT and ATF were able to protect both cortical neurons and SH-SY5Y from H2O2-induced oxidative stress at concetration of ≤10 μM. Cellular uptake of GTT is higher in both cortical neurons and SH-SY5Y as compared to ATF when both cortical neuron and SH-SY5Y were incubated with 10 μM GTT or ATF, respectively for 24 hour. Although primary rat cortical neurons and human neuroblastoma SH-SY5Y were different culture system, the effects of GTT and ATF are similar in both H2O2 –induced culture which strongly suggest that both GTT and ATF act as free radical scavenger to exert their neuroprotective effects.
format	Article
author	Then Sue-Mian, Wan Zurinah Wan NgH, Gapor Mat Top, Musalmah Mazlan,
spellingShingle	Then Sue-Mian, Wan Zurinah Wan NgH, Gapor Mat Top, Musalmah Mazlan, Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures
author_facet	Then Sue-Mian, Wan Zurinah Wan NgH, Gapor Mat Top, Musalmah Mazlan,
author_sort	Then Sue-Mian,
title	Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures
title_short	Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures
title_full	Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures
title_fullStr	Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures
title_full_unstemmed	Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures
title_sort	comparison of the effects of α-tocopherol and γ-tocotrienol against oxidative stress in two different neuronal cultures
publisher	Universiti Kebangsaan Malaysia
publishDate	2010
url	http://journalarticle.ukm.my/338/ http://journalarticle.ukm.my/338/ http://journalarticle.ukm.my/338/1/
first_indexed	2023-09-18T19:30:38Z
last_indexed	2023-09-18T19:30:38Z
_version_	1777404936926003200

Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures

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