Properties of immobilized candida antarctica lipase B on highly macroporous copolymer

In spite of their excellent catalytic properties, enzymes should be improved before their implementation both in industrial and laboratorium scales. Immobilization of enzyme is one of the ways to improve their properties. Candida antarctica lipase B (Cal-B) has been reported in numerous publications...

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Main Authors:	Nurrahmi Handayani, Nemanja Miletic, Katja Loos, Sadijah Achmad, Bumbun Bundjali
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2011
Online Access:	http://journalarticle.ukm.my/2627/ http://journalarticle.ukm.my/2627/ http://journalarticle.ukm.my/2627/1/03_Nurrahmi_Handayani.pdf

id	ukm-2627
recordtype	eprints
spelling	ukm-26272016-12-14T06:32:11Z http://journalarticle.ukm.my/2627/ Properties of immobilized candida antarctica lipase B on highly macroporous copolymer Nurrahmi Handayani, Nemanja Miletic, Katja Loos, Sadijah Achmad, Bumbun Bundjali, In spite of their excellent catalytic properties, enzymes should be improved before their implementation both in industrial and laboratorium scales. Immobilization of enzyme is one of the ways to improve their properties. Candida antarctica lipase B (Cal-B) has been reported in numerous publications to be a particularly useful enzyme catalizing in many type of reaction including regio- and enantio- synthesis. For this case, cross-linking of immobilized Cal-B with 1,2,7,8 diepoxy octane is one of methods that proved significantly more stable from denaturation by heat, organic solvents, and proteolysis than lyophilized powder or soluble enzymes. More over, the aim of this procedure is to improve the activity and reusability of lipase. Enzyme kinetics test was carried out by transesterification reaction between 4-nitrophenyl acetate (pNPA) and methanol by varying substrate concentrations, and the result is immobilized enzymes follows the Michaelis-Menten models and their activity is match with previous experiment. Based on the Vmax values, the immobilized enzymes showed higher activity than the free enzyme. Cross-linking of immobilized lipase indicate that cross-linking by lower concentration of cross-linker, FIC (immobilized lipase that was incubated for 24 h) gave the highest activity and cross-linking by higher concentration of cross-linker, PIC (immobilized lipase that was incubated for 2 h) gives the highest activity. However, pore size and saturation level influenced their activity. Universiti Kebangsaan Malaysia 2011-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2627/1/03_Nurrahmi_Handayani.pdf Nurrahmi Handayani, and Nemanja Miletic, and Katja Loos, and Sadijah Achmad, and Bumbun Bundjali, (2011) Properties of immobilized candida antarctica lipase B on highly macroporous copolymer. Sains Malaysiana, 40 (9). pp. 965-972. ISSN 0126-6039 http:www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In spite of their excellent catalytic properties, enzymes should be improved before their implementation both in industrial and laboratorium scales. Immobilization of enzyme is one of the ways to improve their properties. Candida antarctica lipase B (Cal-B) has been reported in numerous publications to be a particularly useful enzyme catalizing in many type of reaction including regio- and enantio- synthesis. For this case, cross-linking of immobilized Cal-B with 1,2,7,8 diepoxy octane is one of methods that proved significantly more stable from denaturation by heat, organic solvents, and proteolysis than lyophilized powder or soluble enzymes. More over, the aim of this procedure is to improve the activity and reusability of lipase. Enzyme kinetics test was carried out by transesterification reaction between 4-nitrophenyl acetate (pNPA) and methanol by varying substrate concentrations, and the result is immobilized enzymes follows the Michaelis-Menten models and their activity is match with previous experiment. Based on the Vmax values, the immobilized enzymes showed higher activity than the free enzyme. Cross-linking of immobilized lipase indicate that cross-linking by lower concentration of cross-linker, FIC (immobilized lipase that was incubated for 24 h) gave the highest activity and cross-linking by higher concentration of cross-linker, PIC (immobilized lipase that was incubated for 2 h) gives the highest activity. However, pore size and saturation level influenced their activity.
format	Article
author	Nurrahmi Handayani, Nemanja Miletic, Katja Loos, Sadijah Achmad, Bumbun Bundjali,
spellingShingle	Nurrahmi Handayani, Nemanja Miletic, Katja Loos, Sadijah Achmad, Bumbun Bundjali, Properties of immobilized candida antarctica lipase B on highly macroporous copolymer
author_facet	Nurrahmi Handayani, Nemanja Miletic, Katja Loos, Sadijah Achmad, Bumbun Bundjali,
author_sort	Nurrahmi Handayani,
title	Properties of immobilized candida antarctica lipase B on highly macroporous copolymer
title_short	Properties of immobilized candida antarctica lipase B on highly macroporous copolymer
title_full	Properties of immobilized candida antarctica lipase B on highly macroporous copolymer
title_fullStr	Properties of immobilized candida antarctica lipase B on highly macroporous copolymer
title_full_unstemmed	Properties of immobilized candida antarctica lipase B on highly macroporous copolymer
title_sort	properties of immobilized candida antarctica lipase b on highly macroporous copolymer
publisher	Universiti Kebangsaan Malaysia
publishDate	2011
url	http://journalarticle.ukm.my/2627/ http://journalarticle.ukm.my/2627/ http://journalarticle.ukm.my/2627/1/03_Nurrahmi_Handayani.pdf
first_indexed	2023-09-18T19:36:35Z
last_indexed	2023-09-18T19:36:35Z
_version_	1777405311423873024

Properties of immobilized candida antarctica lipase B on highly macroporous copolymer

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