An in vitro study to evaluate the genotoxicity of value-added hydroxyapatite as a bone replacement material

Hydroxyapatite (HA) used for bone replacement is one of the most active areas of ceramic biomaterials research currently. It has been used clinically for the last 20 years due to its excellent biocompatibility, osseoconduction and osseointegration. Many modifications have been done to develop a stro...

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Main Authors:	Akram Hassan, Dasan Swaminathan
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2011
Online Access:	http://journalarticle.ukm.my/2467/ http://journalarticle.ukm.my/2467/ http://journalarticle.ukm.my/2467/1/12_Akram.pdf

id	ukm-2467
recordtype	eprints
spelling	ukm-24672016-12-14T06:31:42Z http://journalarticle.ukm.my/2467/ An in vitro study to evaluate the genotoxicity of value-added hydroxyapatite as a bone replacement material Akram Hassan, Dasan Swaminathan, Hydroxyapatite (HA) used for bone replacement is one of the most active areas of ceramic biomaterials research currently. It has been used clinically for the last 20 years due to its excellent biocompatibility, osseoconduction and osseointegration. Many modifications have been done to develop a stronger, tougher and biocompatible ceramic biomaterial because pure HA is brittle. Researchers in Universiti Sains Malaysia had developed this value added HA that is stronger and less brittle compared to pure HA. The objective of this in vitro study was to evaluate the genotoxic characteristic of the value added HA based material by using Bacterial Reverse Mutation Assay (Ames test). The Bacterial Reverse Mutation Assay of HA was performed on Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and Escherichia coli strain WP2 uvrA using the preincubation method in the presence and absence of an exogenous metabolic activation system. All the bacterial tester strains treated with and without S9 Mix showed no increase of revertant colonies with increase in concentration of test substance for both the dose finding test and the main test. The number of revertant colonies was less than twice that of the solvent control for all the five bacterial strains and this was reproducible for both the dose finding test and the main test. The numbers of revertant colonies in the negative and positive controls were within the background data of our laboratory. In conclusion the results of the tests showed that the value added HA was considered to have no reverse mutagenic potential under the present test conditions Universiti Kebangsaan Malaysia 2011-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2467/1/12_Akram.pdf Akram Hassan, and Dasan Swaminathan, (2011) An in vitro study to evaluate the genotoxicity of value-added hydroxyapatite as a bone replacement material. Sains Malaysiana, 40 (2). pp. 163-171. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Hydroxyapatite (HA) used for bone replacement is one of the most active areas of ceramic biomaterials research currently. It has been used clinically for the last 20 years due to its excellent biocompatibility, osseoconduction and osseointegration. Many modifications have been done to develop a stronger, tougher and biocompatible ceramic biomaterial because pure HA is brittle. Researchers in Universiti Sains Malaysia had developed this value added HA that is stronger and less brittle compared to pure HA. The objective of this in vitro study was to evaluate the genotoxic characteristic of the value added HA based material by using Bacterial Reverse Mutation Assay (Ames test). The Bacterial Reverse Mutation Assay of HA was performed on Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and Escherichia coli strain WP2 uvrA using the preincubation method in the presence and absence of an exogenous metabolic activation system. All the bacterial tester strains treated with and without S9 Mix showed no increase of revertant colonies with increase in concentration of test substance for both the dose finding test and the main test. The number of revertant colonies was less than twice that of the solvent control for all the five bacterial strains and this was reproducible for both the dose finding test and the main test. The numbers of revertant colonies in the negative and positive controls were within the background data of our laboratory. In conclusion the results of the tests showed that the value added HA was considered to have no reverse mutagenic potential under the present test conditions
format	Article
author	Akram Hassan, Dasan Swaminathan,
spellingShingle	Akram Hassan, Dasan Swaminathan, An in vitro study to evaluate the genotoxicity of value-added hydroxyapatite as a bone replacement material
author_facet	Akram Hassan, Dasan Swaminathan,
author_sort	Akram Hassan,
title	An in vitro study to evaluate the genotoxicity of value-added hydroxyapatite as a bone replacement material
title_short	An in vitro study to evaluate the genotoxicity of value-added hydroxyapatite as a bone replacement material
title_full	An in vitro study to evaluate the genotoxicity of value-added hydroxyapatite as a bone replacement material
title_fullStr	An in vitro study to evaluate the genotoxicity of value-added hydroxyapatite as a bone replacement material
title_full_unstemmed	An in vitro study to evaluate the genotoxicity of value-added hydroxyapatite as a bone replacement material
title_sort	in vitro study to evaluate the genotoxicity of value-added hydroxyapatite as a bone replacement material
publisher	Universiti Kebangsaan Malaysia
publishDate	2011
url	http://journalarticle.ukm.my/2467/ http://journalarticle.ukm.my/2467/ http://journalarticle.ukm.my/2467/1/12_Akram.pdf
first_indexed	2023-09-18T19:36:08Z
last_indexed	2023-09-18T19:36:08Z
_version_	1777405283289530368

An in vitro study to evaluate the genotoxicity of value-added hydroxyapatite as a bone replacement material

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