In Vivo production of nosema bombycisSpores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions

Diamondback moth (DBM), Plutella xylostella and beet armyworm (BAW), Spodoptera exigua are major insect pests of crucifer crops found worldwide. Since these pests are highly resistant to many chemical insecticides, using biological control agent(s) or biopesticides might be a good choice to overcome...

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Main Authors:	Syarafina Ramli, Zainal-Abidin .B.A.H, Idris .A.B
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2011
Online Access:	http://journalarticle.ukm.my/2389/ http://journalarticle.ukm.my/2389/ http://journalarticle.ukm.my/2389/1/05_Syarafina.pdf

id	ukm-2389
recordtype	eprints
spelling	ukm-23892016-12-14T06:31:29Z http://journalarticle.ukm.my/2389/ In Vivo production of nosema bombycisSpores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions Syarafina Ramli, Zainal-Abidin .B.A.H, Idris .A.B, Diamondback moth (DBM), Plutella xylostella and beet armyworm (BAW), Spodoptera exigua are major insect pests of crucifer crops found worldwide. Since these pests are highly resistant to many chemical insecticides, using biological control agent(s) or biopesticides might be a good choice to overcome this problem. Nosema bombycis is a microsporidial pathogen with the potential to control insect pests. This study was aimed at producing N. bombycis spores in vivo and to test their efficacies against DBM and BAW in laboratory conditions. Production of this pathogen was carried out by feeding both DBM and BAW larval instars with artificial diet inoculated with viable spores of N. bombycis. Spore concentrations in the larvae were determined and accumulated. If a concentration of 1.56×108 spores/mL was obtained, the production was continued. For laboratory efficacy tests, artificial diets with different spore concentrations namely 1×104, 1×105 and 1×106 spores/μL were fed to different larval instar. Mortality rates were analysed using the Two-way ANOVA test. For both DBM and BAW, third instar showed the highest mean mortality rates and the mortality in each instars were significantly different (P<0.05). The concentration 1×106 spores/μL caused the highest mean mortality rates and each concentration resulted in significantly different mortality (P<0.05). However, there was no interaction between both factors, where a combination of spore concentrations and instars did not show any significant differences (P>0.05). These results indicated that third instar larvae for both species and 1×106 spores/μL treatments demonstrated the highest efficacy rates Universiti Kebangsaan Malaysia 2011-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2389/1/05_Syarafina.pdf Syarafina Ramli, and Zainal-Abidin .B.A.H, and Idris .A.B, (2011) In Vivo production of nosema bombycisSpores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions. Sains Malaysiana, 40 (4). pp. 311-316. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Diamondback moth (DBM), Plutella xylostella and beet armyworm (BAW), Spodoptera exigua are major insect pests of crucifer crops found worldwide. Since these pests are highly resistant to many chemical insecticides, using biological control agent(s) or biopesticides might be a good choice to overcome this problem. Nosema bombycis is a microsporidial pathogen with the potential to control insect pests. This study was aimed at producing N. bombycis spores in vivo and to test their efficacies against DBM and BAW in laboratory conditions. Production of this pathogen was carried out by feeding both DBM and BAW larval instars with artificial diet inoculated with viable spores of N. bombycis. Spore concentrations in the larvae were determined and accumulated. If a concentration of 1.56×108 spores/mL was obtained, the production was continued. For laboratory efficacy tests, artificial diets with different spore concentrations namely 1×104, 1×105 and 1×106 spores/μL were fed to different larval instar. Mortality rates were analysed using the Two-way ANOVA test. For both DBM and BAW, third instar showed the highest mean mortality rates and the mortality in each instars were significantly different (P<0.05). The concentration 1×106 spores/μL caused the highest mean mortality rates and each concentration resulted in significantly different mortality (P<0.05). However, there was no interaction between both factors, where a combination of spore concentrations and instars did not show any significant differences (P>0.05). These results indicated that third instar larvae for both species and 1×106 spores/μL treatments demonstrated the highest efficacy rates
format	Article
author	Syarafina Ramli, Zainal-Abidin .B.A.H, Idris .A.B,
spellingShingle	Syarafina Ramli, Zainal-Abidin .B.A.H, Idris .A.B, In Vivo production of nosema bombycisSpores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions
author_facet	Syarafina Ramli, Zainal-Abidin .B.A.H, Idris .A.B,
author_sort	Syarafina Ramli,
title	In Vivo production of nosema bombycisSpores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions
title_short	In Vivo production of nosema bombycisSpores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions
title_full	In Vivo production of nosema bombycisSpores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions
title_fullStr	In Vivo production of nosema bombycisSpores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions
title_full_unstemmed	In Vivo production of nosema bombycisSpores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions
title_sort	in vivo production of nosema bombycisspores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions
publisher	Universiti Kebangsaan Malaysia
publishDate	2011
url	http://journalarticle.ukm.my/2389/ http://journalarticle.ukm.my/2389/ http://journalarticle.ukm.my/2389/1/05_Syarafina.pdf
first_indexed	2023-09-18T19:35:59Z
last_indexed	2023-09-18T19:35:59Z
_version_	1777405273622708224

In Vivo production of nosema bombycisSpores and their efficacies against diamondback moth and beet armyworm larvae in laboratory conditions

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