Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells

Stem cell is defined as the ability of the cell to proliferate themselves and differentiate into more than one type of cells. Human mononucleated cell (MN cell) is a suspension cell that was isolated from peripheral blood that was originated from monocyte-machrophage lineage or hematopoietic stem ce...

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Main Authors:	Siti Afeefah Mohamad Yusof, Rohaya Megat Abdul Wahab, Sahidan Senafi, Zaidah Zainal Ariffin, Mohamad Abdul Razak, Shahrul Hisham Zainal Ariffin
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2011
Online Access:	http://journalarticle.ukm.my/2387/ http://journalarticle.ukm.my/2387/ http://journalarticle.ukm.my/2387/1/04_Siti_Afeefah.pdf

id	ukm-2387
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spelling	ukm-23872016-12-14T06:31:28Z http://journalarticle.ukm.my/2387/ Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells Siti Afeefah Mohamad Yusof, Rohaya Megat Abdul Wahab, Sahidan Senafi,, Zaidah Zainal Ariffin, Mohamad Abdul Razak, Shahrul Hisham Zainal Ariffin, Stem cell is defined as the ability of the cell to proliferate themselves and differentiate into more than one type of cells. Human mononucleated cell (MN cell) is a suspension cell that was isolated from peripheral blood that was originated from monocyte-machrophage lineage or hematopoietic stem cells. The cells were cultured for 30 days in complete media (CM) which consist of Alpha Minimal Essential Medium (αMEM) with 2% (v/v) Penicillin-Streptomycin and 10% (v/v) Newborn Calf Serum (NBCS). The respective cells were differentiated at day 7 after in vitro proliferation in CM into osteoblastic cells by adding ascorbic acid and β-glycerophosphate. In addition, human recombinant Receptor Activator of Nuclear Factor-β Ligand (hrRANKL) and human recombinant Macrophage-Colony Stimulating Factor (hrM-CSF) were added to induce osteoclastic differentiation of MN cells. Cells that were cultured in CM served as a control and were subjected to the same approach as differentiated cells. The 30 days cultured cells in CM showed a significant increment (p < 0.05) of viable cells compared to day 0 (n=3). The specific activity of Alkaline Phosphatase (ALP) for osteoblast differentiation and Tartrate Resistant Acid Phosphatase (TRAP) for osteoclast differentiation were evaluated via biochemical assay until day 14 and day 10 for osteoblast and osteoclast sample, respectively. ALP and TRAP enzyme showed a significant increment (p < 0.05) after 14 and 10 days of differentiation compared to control cells. As a conclusion, human mononucleated cells are believed to have the potential to be defined as a multipotent stem cell based on their fulfillment of stem cell characteristics Universiti Kebangsaan Malaysia 2011-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2387/1/04_Siti_Afeefah.pdf Siti Afeefah Mohamad Yusof, and Rohaya Megat Abdul Wahab, and Sahidan Senafi,, and Zaidah Zainal Ariffin, and Mohamad Abdul Razak, and Shahrul Hisham Zainal Ariffin, (2011) Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells. Sains Malaysiana, 40 (4). pp. 305-309. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Stem cell is defined as the ability of the cell to proliferate themselves and differentiate into more than one type of cells. Human mononucleated cell (MN cell) is a suspension cell that was isolated from peripheral blood that was originated from monocyte-machrophage lineage or hematopoietic stem cells. The cells were cultured for 30 days in complete media (CM) which consist of Alpha Minimal Essential Medium (αMEM) with 2% (v/v) Penicillin-Streptomycin and 10% (v/v) Newborn Calf Serum (NBCS). The respective cells were differentiated at day 7 after in vitro proliferation in CM into osteoblastic cells by adding ascorbic acid and β-glycerophosphate. In addition, human recombinant Receptor Activator of Nuclear Factor-β Ligand (hrRANKL) and human recombinant Macrophage-Colony Stimulating Factor (hrM-CSF) were added to induce osteoclastic differentiation of MN cells. Cells that were cultured in CM served as a control and were subjected to the same approach as differentiated cells. The 30 days cultured cells in CM showed a significant increment (p < 0.05) of viable cells compared to day 0 (n=3). The specific activity of Alkaline Phosphatase (ALP) for osteoblast differentiation and Tartrate Resistant Acid Phosphatase (TRAP) for osteoclast differentiation were evaluated via biochemical assay until day 14 and day 10 for osteoblast and osteoclast sample, respectively. ALP and TRAP enzyme showed a significant increment (p < 0.05) after 14 and 10 days of differentiation compared to control cells. As a conclusion, human mononucleated cells are believed to have the potential to be defined as a multipotent stem cell based on their fulfillment of stem cell characteristics
format	Article
author	Siti Afeefah Mohamad Yusof, Rohaya Megat Abdul Wahab, Sahidan Senafi,, Zaidah Zainal Ariffin, Mohamad Abdul Razak, Shahrul Hisham Zainal Ariffin,
spellingShingle	Siti Afeefah Mohamad Yusof, Rohaya Megat Abdul Wahab, Sahidan Senafi,, Zaidah Zainal Ariffin, Mohamad Abdul Razak, Shahrul Hisham Zainal Ariffin, Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells
author_facet	Siti Afeefah Mohamad Yusof, Rohaya Megat Abdul Wahab, Sahidan Senafi,, Zaidah Zainal Ariffin, Mohamad Abdul Razak, Shahrul Hisham Zainal Ariffin,
author_sort	Siti Afeefah Mohamad Yusof,
title	Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells
title_short	Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells
title_full	Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells
title_fullStr	Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells
title_full_unstemmed	Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells
title_sort	proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells
publisher	Universiti Kebangsaan Malaysia
publishDate	2011
url	http://journalarticle.ukm.my/2387/ http://journalarticle.ukm.my/2387/ http://journalarticle.ukm.my/2387/1/04_Siti_Afeefah.pdf
first_indexed	2023-09-18T19:35:56Z
last_indexed	2023-09-18T19:35:56Z
_version_	1777405270623780864

Proliferation and biochemical analyses of osteoblast/osteoclast differentiation from human mononucleated cells

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