Gamma-tocotrienol alters protein expression of HepG2 cell line

Gamma-tocotrienol (GTT) has been shown to exhibit significant antitumor activity in a variety of tumor cells. Previous findings have demonstrated that GTT had antiprolifera-tive effects on a liver cancer cell line (HepG2) with an IC50 value of 170μM. In this study, two dimensional gel electrophoresi...

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Main Authors:	Farahani ARS, Zakiah J, Abdul Rahman M, Karsani SA, Wan Ngah WZ
Format:	Article
Language:	English
Published:	Penerbit UKM 2008
Online Access:	http://journalarticle.ukm.my/2032/ http://journalarticle.ukm.my/2032/ http://journalarticle.ukm.my/2032/1/06-256-262_%28MS_067%29.pdf

id	ukm-2032
recordtype	eprints
spelling	ukm-20322016-12-14T06:30:43Z http://journalarticle.ukm.my/2032/ Gamma-tocotrienol alters protein expression of HepG2 cell line Farahani ARS, Zakiah J, Abdul Rahman M, Karsani SA, Wan Ngah WZ, Gamma-tocotrienol (GTT) has been shown to exhibit significant antitumor activity in a variety of tumor cells. Previous findings have demonstrated that GTT had antiprolifera-tive effects on a liver cancer cell line (HepG2) with an IC50 value of 170μM. In this study, two dimensional gel electrophoresis (2DE) was used to determine changes in protein expression in HepG2 cell line following treatment with GTT. The ultimate aim is to identify the possible molecular mechanisms involved in GTT antitumor activity. This study is focused on obtaining a 2DE protein profile for HepG2 cell line with and without GTT treatment. In the preliminary analysis of the resulting 2DE profiles, 18 protein spots were found to be differentially expressed in cells treated with GTT. This observa-tion is confirmed by extending the analysis to a larger sample size. By studying the effects of GTT treatment on differential protein expression in HepG2 cells the underly-ing mechanisms involved in the antitumor activity of GTT may be elucidated Penerbit UKM 2008 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2032/1/06-256-262_%28MS_067%29.pdf Farahani ARS, and Zakiah J, and Abdul Rahman M, and Karsani SA, and Wan Ngah WZ, (2008) Gamma-tocotrienol alters protein expression of HepG2 cell line. Medicine & Health, 3 (2). pp. 256-262. ISSN 1823-2140 http://www.ppukm.ukm.my/ukmmcjournal/index.php
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Gamma-tocotrienol (GTT) has been shown to exhibit significant antitumor activity in a variety of tumor cells. Previous findings have demonstrated that GTT had antiprolifera-tive effects on a liver cancer cell line (HepG2) with an IC50 value of 170μM. In this study, two dimensional gel electrophoresis (2DE) was used to determine changes in protein expression in HepG2 cell line following treatment with GTT. The ultimate aim is to identify the possible molecular mechanisms involved in GTT antitumor activity. This study is focused on obtaining a 2DE protein profile for HepG2 cell line with and without GTT treatment. In the preliminary analysis of the resulting 2DE profiles, 18 protein spots were found to be differentially expressed in cells treated with GTT. This observa-tion is confirmed by extending the analysis to a larger sample size. By studying the effects of GTT treatment on differential protein expression in HepG2 cells the underly-ing mechanisms involved in the antitumor activity of GTT may be elucidated
format	Article
author	Farahani ARS, Zakiah J, Abdul Rahman M, Karsani SA, Wan Ngah WZ,
spellingShingle	Farahani ARS, Zakiah J, Abdul Rahman M, Karsani SA, Wan Ngah WZ, Gamma-tocotrienol alters protein expression of HepG2 cell line
author_facet	Farahani ARS, Zakiah J, Abdul Rahman M, Karsani SA, Wan Ngah WZ,
author_sort	Farahani ARS,
title	Gamma-tocotrienol alters protein expression of HepG2 cell line
title_short	Gamma-tocotrienol alters protein expression of HepG2 cell line
title_full	Gamma-tocotrienol alters protein expression of HepG2 cell line
title_fullStr	Gamma-tocotrienol alters protein expression of HepG2 cell line
title_full_unstemmed	Gamma-tocotrienol alters protein expression of HepG2 cell line
title_sort	gamma-tocotrienol alters protein expression of hepg2 cell line
publisher	Penerbit UKM
publishDate	2008
url	http://journalarticle.ukm.my/2032/ http://journalarticle.ukm.my/2032/ http://journalarticle.ukm.my/2032/1/06-256-262_%28MS_067%29.pdf
first_indexed	2023-09-18T19:35:00Z
last_indexed	2023-09-18T19:35:00Z
_version_	1777405211863678976

Gamma-tocotrienol alters protein expression of HepG2 cell line

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