$Melanogenesis inhibition by palm tocotrienol rich fraction in cellular ageing$

Melanogenesis inhibition by palm tocotrienol rich fraction in cellular ageing

Melanin is the pigment that determines skin color. Melanin synthesis is catalysed by the enzyme tyrosinase and is controlled by TYR, TYRP1 and TYRP2 genes. In this study, tocotrienol rich fraction (TRF) was used to inhibit melanin synthesis. TRF contains 75% α-tocotrienol and 25% tocopherol. The obj...

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Main Authors:	Suzana M, Nur Nadia MA, Zahariah I, Chua KH, Yasmin Anum MY, Wan Zurinah WN
Format:	Article
Language:	English
Published:	Penerbit UKM 2009
Online Access:	http://journalarticle.ukm.my/1916/ http://journalarticle.ukm.my/1916/ http://journalarticle.ukm.my/1916/1/4-Page_1-7_%28MS_074%29.pdf

id	ukm-1916
recordtype	eprints
spelling	ukm-19162016-12-14T06:30:26Z http://journalarticle.ukm.my/1916/ Melanogenesis inhibition by palm tocotrienol rich fraction in cellular ageing Suzana M, Nur Nadia MA, Zahariah I, Chua KH, Yasmin Anum MY, Wan Zurinah WN, Melanin is the pigment that determines skin color. Melanin synthesis is catalysed by the enzyme tyrosinase and is controlled by TYR, TYRP1 and TYRP2 genes. In this study, tocotrienol rich fraction (TRF) was used to inhibit melanin synthesis. TRF contains 75% α-tocotrienol and 25% tocopherol. The objective of this study was to determine the effect of tocotrienol rich fraction (TRF) on melanin synthesis by determining melanin content and expression of genes involved in the regulation of melanin synthesis in skin melanocytes. Melanin synthesis was studied by determining melanin level and tyrosinase enzyme activity, while expression of TYR, TYRP1 and TYRP2 genes was determined by quantitative real time reverse transcriptase poly-merase chain reaction (real time RT-PCR). Primary culture of skin melanocytes were divided into two groups; control and cells that were treated with 500 μg/ml tocotrienol rich fraction for 24 hours. Our results showed that there was a reduction in melanin content and tyrosinase activity in skin melanocytes treated with tocotrienol rich fraction compared to the control (p<0.05). Expression of TYRP2 gene in melanocytes treated with tocotrienol rich fraction was also decreased (p<0.05) compared to the control. In conclusion, palm tocotrienol rich fraction has anti pigmentation property that inhibits melanin synthesis in cellular aging by inhibiting tyrosinase activity and down regulating TYRP2 gene expression. Penerbit UKM 2009 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/1916/1/4-Page_1-7_%28MS_074%29.pdf Suzana M, and Nur Nadia MA, and Zahariah I, and Chua KH, and Yasmin Anum MY, and Wan Zurinah WN, (2009) Melanogenesis inhibition by palm tocotrienol rich fraction in cellular ageing. Medicine & Health, 4 (1). pp. 25-34. ISSN 1823-2140 http://www.ppukm.ukm.my/ukmmcjournal/index.php
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Melanin is the pigment that determines skin color. Melanin synthesis is catalysed by the enzyme tyrosinase and is controlled by TYR, TYRP1 and TYRP2 genes. In this study, tocotrienol rich fraction (TRF) was used to inhibit melanin synthesis. TRF contains 75% α-tocotrienol and 25% tocopherol. The objective of this study was to determine the effect of tocotrienol rich fraction (TRF) on melanin synthesis by determining melanin content and expression of genes involved in the regulation of melanin synthesis in skin melanocytes. Melanin synthesis was studied by determining melanin level and tyrosinase enzyme activity, while expression of TYR, TYRP1 and TYRP2 genes was determined by quantitative real time reverse transcriptase poly-merase chain reaction (real time RT-PCR). Primary culture of skin melanocytes were divided into two groups; control and cells that were treated with 500 μg/ml tocotrienol rich fraction for 24 hours. Our results showed that there was a reduction in melanin content and tyrosinase activity in skin melanocytes treated with tocotrienol rich fraction compared to the control (p<0.05). Expression of TYRP2 gene in melanocytes treated with tocotrienol rich fraction was also decreased (p<0.05) compared to the control. In conclusion, palm tocotrienol rich fraction has anti pigmentation property that inhibits melanin synthesis in cellular aging by inhibiting tyrosinase activity and down regulating TYRP2 gene expression.
format	Article
author	Suzana M, Nur Nadia MA, Zahariah I, Chua KH, Yasmin Anum MY, Wan Zurinah WN,
spellingShingle	Suzana M, Nur Nadia MA, Zahariah I, Chua KH, Yasmin Anum MY, Wan Zurinah WN, Melanogenesis inhibition by palm tocotrienol rich fraction in cellular ageing
author_facet	Suzana M, Nur Nadia MA, Zahariah I, Chua KH, Yasmin Anum MY, Wan Zurinah WN,
author_sort	Suzana M,
title	Melanogenesis inhibition by palm tocotrienol rich fraction in cellular ageing
title_short	Melanogenesis inhibition by palm tocotrienol rich fraction in cellular ageing
title_full	Melanogenesis inhibition by palm tocotrienol rich fraction in cellular ageing
title_fullStr	Melanogenesis inhibition by palm tocotrienol rich fraction in cellular ageing
title_full_unstemmed	Melanogenesis inhibition by palm tocotrienol rich fraction in cellular ageing
title_sort	melanogenesis inhibition by palm tocotrienol rich fraction in cellular ageing
publisher	Penerbit UKM
publishDate	2009
url	http://journalarticle.ukm.my/1916/ http://journalarticle.ukm.my/1916/ http://journalarticle.ukm.my/1916/1/4-Page_1-7_%28MS_074%29.pdf
first_indexed	2023-09-18T19:34:42Z
last_indexed	2023-09-18T19:34:42Z
_version_	1777405192639086592

Melanogenesis inhibition by palm tocotrienol rich fraction in cellular ageing

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