Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques

The reciprocal translocation t(9;22)(q34;q11) which gives rise to the Philadelphia (Ph1) chromosome and BCR/ABL fusion gene, plays a pivotal role in the diagnosis and pathogenesis of chronic myeloid leukemia (CML). In this study, we evaluated the role of fluorescence in situ hybridisation (FISH) in...

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Main Authors:	Reena RMZ, Julia Munchar MJ, Salwati S, Hamidah NH, Zubaidah Z, Sharifah NA, Cheong SK
Format:	Article
Language:	English
Published:	Penerbit UKM 2006
Online Access:	http://journalarticle.ukm.my/1829/ http://journalarticle.ukm.my/1829/ http://journalarticle.ukm.my/1829/1/02._5-13_%28MH_006%29.pdf

id	ukm-1829
recordtype	eprints
spelling	ukm-18292016-12-14T06:30:18Z http://journalarticle.ukm.my/1829/ Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques Reena RMZ, Julia Munchar MJ, Salwati S,, Hamidah NH, Zubaidah Z, Sharifah NA, Cheong SK, The reciprocal translocation t(9;22)(q34;q11) which gives rise to the Philadelphia (Ph1) chromosome and BCR/ABL fusion gene, plays a pivotal role in the diagnosis and pathogenesis of chronic myeloid leukemia (CML). In this study, we evaluated the role of fluorescence in situ hybridisation (FISH) in detecting the BCR/ABL rearrangement in CML patients. The sensitivity, specificity and detection rate of BCR/ABL gene using FISH, PCR and conventional cytogenetics (karyotyping) methods were also compared. 18 bone marrow samples of patients with clinically diagnosed CML and suspected of CML were collected. The sensitivity, specificity and positive predictive values of FISH were altogether Penerbit UKM 2006 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/1829/1/02._5-13_%28MH_006%29.pdf Reena RMZ, and Julia Munchar MJ, and Salwati S,, and Hamidah NH, and Zubaidah Z, and Sharifah NA, and Cheong SK, (2006) Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques. Medicine & Health, 1 (1). pp. 5-13. ISSN 1823-2140 http://www.ppukm.ukm.my/ukmmcjournal/index.php
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The reciprocal translocation t(9;22)(q34;q11) which gives rise to the Philadelphia (Ph1) chromosome and BCR/ABL fusion gene, plays a pivotal role in the diagnosis and pathogenesis of chronic myeloid leukemia (CML). In this study, we evaluated the role of fluorescence in situ hybridisation (FISH) in detecting the BCR/ABL rearrangement in CML patients. The sensitivity, specificity and detection rate of BCR/ABL gene using FISH, PCR and conventional cytogenetics (karyotyping) methods were also compared. 18 bone marrow samples of patients with clinically diagnosed CML and suspected of CML were collected. The sensitivity, specificity and positive predictive values of FISH were altogether
format	Article
author	Reena RMZ, Julia Munchar MJ, Salwati S,, Hamidah NH, Zubaidah Z, Sharifah NA, Cheong SK,
spellingShingle	Reena RMZ, Julia Munchar MJ, Salwati S,, Hamidah NH, Zubaidah Z, Sharifah NA, Cheong SK, Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques
author_facet	Reena RMZ, Julia Munchar MJ, Salwati S,, Hamidah NH, Zubaidah Z, Sharifah NA, Cheong SK,
author_sort	Reena RMZ,
title	Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques
title_short	Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques
title_full	Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques
title_fullStr	Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques
title_full_unstemmed	Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques
title_sort	detection of bcr/abl gene in chronic myeloid leukaemia: comparison of fluorescence in situ hybridisation (fish), conventional cytogenetics and polymerase chain reaction (pcr) techniques
publisher	Penerbit UKM
publishDate	2006
url	http://journalarticle.ukm.my/1829/ http://journalarticle.ukm.my/1829/ http://journalarticle.ukm.my/1829/1/02._5-13_%28MH_006%29.pdf
first_indexed	2023-09-18T19:34:27Z
last_indexed	2023-09-18T19:34:27Z
_version_	1777405177373917184

Detection of BCR/ABL gene in chronic Myeloid Leukaemia: comparison of Fluorescence in situ Hybridisation (FISH), conventional Cytogenetics and Polymerase Chain Reaction (PCR) techniques

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