Role of different Salmonella enterica serotypes in THP-1 macrophage-derived foam cell autophagy and inflammatory response

Infected aortic aneurysm is a rare but life-threatening condition. Intimal injury, especially in arteries with atherosclerotic plaques, is vulnerable to Salmonella species. In this study, THP-1 macrophage-derived foam cells were used to mimic the atherosclerotic plaques. We investigated the role of...

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Main Authors:	Wong, Min Yi, Tseng, Yuan-Hsi, Huang, Tsung-Yu, Chiu, Cheng-Hsun, Chen, Chyi-Liang, Huang, Yao-Kuang
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/14406/ http://journalarticle.ukm.my/14406/ http://journalarticle.ukm.my/14406/1/21%20Min%20Yi%20Wong.pdf

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spelling	ukm-144062020-03-10T02:33:51Z http://journalarticle.ukm.my/14406/ Role of different Salmonella enterica serotypes in THP-1 macrophage-derived foam cell autophagy and inflammatory response Wong, Min Yi Tseng, Yuan-Hsi Huang, Tsung-Yu Chiu, Cheng-Hsun Chen, Chyi-Liang Huang, Yao-Kuang Infected aortic aneurysm is a rare but life-threatening condition. Intimal injury, especially in arteries with atherosclerotic plaques, is vulnerable to Salmonella species. In this study, THP-1 macrophage-derived foam cells were used to mimic the atherosclerotic plaques. We investigated the role of different Salmonella serotype infections in foam cell autophagy and inflammatory response. Three different serotypes of Salmonella enterica-Typhimurium, Enteritidis, and Choleraesuis - with or without a virulence plasmid were used in this study. The foam cell model was established and observed under confocal microscopy. The expression of autophagy and inflammasome-related proteins after Salmonella infection was detected by Western blotting, and the secretion of the proinflammatory cytokines interleukin-1β and IL-18 was detected using ELISA. The plasmid-bearing S. enteritidis OU7130- and plasmid-less S. choleraesuis OU7266-infected foam cells both showed a lower expression of beclin-1 at 0.5 h post-infection (hpi) and p62 at 2 hpi as well as a higher expression of caspase-1 at 2 hpi than other infected cells. Moreover, infection of OU7266 induced the highest IL-1β and IL-18 production among the tested strains. In conclusion, the virulence plasmid of S. enteritidis OU7130 induced foam cell inflammatory responses, whereas that of S. choleraesuis played an opposite role. The different serotype of Salmonella may impact the infected aortic aneurysms through regulating inflammatory balance of the macrophage and foam cells in the atherosclerotic plaque. Penerbit Universiti Kebangsaan Malaysia 2019-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14406/1/21%20Min%20Yi%20Wong.pdf Wong, Min Yi and Tseng, Yuan-Hsi and Huang, Tsung-Yu and Chiu, Cheng-Hsun and Chen, Chyi-Liang and Huang, Yao-Kuang (2019) Role of different Salmonella enterica serotypes in THP-1 macrophage-derived foam cell autophagy and inflammatory response. Sains Malaysiana, 48 (10). pp. 2249-2256. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil10_2019/KandunganJilid48Bil10_2019.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Infected aortic aneurysm is a rare but life-threatening condition. Intimal injury, especially in arteries with atherosclerotic plaques, is vulnerable to Salmonella species. In this study, THP-1 macrophage-derived foam cells were used to mimic the atherosclerotic plaques. We investigated the role of different Salmonella serotype infections in foam cell autophagy and inflammatory response. Three different serotypes of Salmonella enterica-Typhimurium, Enteritidis, and Choleraesuis - with or without a virulence plasmid were used in this study. The foam cell model was established and observed under confocal microscopy. The expression of autophagy and inflammasome-related proteins after Salmonella infection was detected by Western blotting, and the secretion of the proinflammatory cytokines interleukin-1β and IL-18 was detected using ELISA. The plasmid-bearing S. enteritidis OU7130- and plasmid-less S. choleraesuis OU7266-infected foam cells both showed a lower expression of beclin-1 at 0.5 h post-infection (hpi) and p62 at 2 hpi as well as a higher expression of caspase-1 at 2 hpi than other infected cells. Moreover, infection of OU7266 induced the highest IL-1β and IL-18 production among the tested strains. In conclusion, the virulence plasmid of S. enteritidis OU7130 induced foam cell inflammatory responses, whereas that of S. choleraesuis played an opposite role. The different serotype of Salmonella may impact the infected aortic aneurysms through regulating inflammatory balance of the macrophage and foam cells in the atherosclerotic plaque.
format	Article
author	Wong, Min Yi Tseng, Yuan-Hsi Huang, Tsung-Yu Chiu, Cheng-Hsun Chen, Chyi-Liang Huang, Yao-Kuang
spellingShingle	Wong, Min Yi Tseng, Yuan-Hsi Huang, Tsung-Yu Chiu, Cheng-Hsun Chen, Chyi-Liang Huang, Yao-Kuang Role of different Salmonella enterica serotypes in THP-1 macrophage-derived foam cell autophagy and inflammatory response
author_facet	Wong, Min Yi Tseng, Yuan-Hsi Huang, Tsung-Yu Chiu, Cheng-Hsun Chen, Chyi-Liang Huang, Yao-Kuang
author_sort	Wong, Min Yi
title	Role of different Salmonella enterica serotypes in THP-1 macrophage-derived foam cell autophagy and inflammatory response
title_short	Role of different Salmonella enterica serotypes in THP-1 macrophage-derived foam cell autophagy and inflammatory response
title_full	Role of different Salmonella enterica serotypes in THP-1 macrophage-derived foam cell autophagy and inflammatory response
title_fullStr	Role of different Salmonella enterica serotypes in THP-1 macrophage-derived foam cell autophagy and inflammatory response
title_full_unstemmed	Role of different Salmonella enterica serotypes in THP-1 macrophage-derived foam cell autophagy and inflammatory response
title_sort	role of different salmonella enterica serotypes in thp-1 macrophage-derived foam cell autophagy and inflammatory response
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/14406/ http://journalarticle.ukm.my/14406/ http://journalarticle.ukm.my/14406/1/21%20Min%20Yi%20Wong.pdf
first_indexed	2023-09-18T20:07:00Z
last_indexed	2023-09-18T20:07:00Z
_version_	1777407225299468288

Role of different Salmonella enterica serotypes in THP-1 macrophage-derived foam cell autophagy and inflammatory response

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