$Semi-purified fraction of Clinacanthus nutans induced apoptosis in human cervical cancer, SiHa cells via up-regulation of bax and down-regulation of Bcl-2$

Semi-purified fraction of Clinacanthus nutans induced apoptosis in human cervical cancer, SiHa cells via up-regulation of bax and down-regulation of Bcl-2

Cervical cancer is a leading cause of death in women, worldwide, and second in Malaysia. Nowadays, modern cancer treatment comes with negative and adverse side effects to the patients. Traditional medicinal plants such as Clinacanthus nutans has been locally recognized for its medicinal properties a...

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Main Authors:	Nik Aina Syazana Nik Zainuddin, Hussin Muhammad, Nik Fakhuruddin Nik Hassan, Nor Hayati Othman, Yusmazura Zakaria
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/14354/ http://journalarticle.ukm.my/14354/ http://journalarticle.ukm.my/14354/1/21%20Nik%20Aina%20Syazana%20Nik%20Zainuddin.pdf

id	ukm-14354
recordtype	eprints
spelling	ukm-143542020-03-03T08:05:48Z http://journalarticle.ukm.my/14354/ Semi-purified fraction of Clinacanthus nutans induced apoptosis in human cervical cancer, SiHa cells via up-regulation of bax and down-regulation of Bcl-2 Nik Aina Syazana Nik Zainuddin, Hussin Muhammad, Nik Fakhuruddin Nik Hassan, Nor Hayati Othman, Yusmazura Zakaria, Cervical cancer is a leading cause of death in women, worldwide, and second in Malaysia. Nowadays, modern cancer treatment comes with negative and adverse side effects to the patients. Traditional medicinal plants such as Clinacanthus nutans has been locally recognized for its medicinal properties and was claimed for cancer treatment. According to our pilot study, a fraction of C. nutans has shown a potent inhibition on human cervical cancer cells, SiHa, in vitro. Hence, this study aimed to investigate the underlying anticancer mechanism of a semi-purified fraction (SF1) of C. nutans towards cervical cancer cells. Bioassay guided fractionation and further purification was carried out. All collected fractions of C. nutans were examined for cytotoxicity activity towards HeLa, SiHa and NIH cells by MTT Assay. SF1 exhibited better cytotoxic activity with best growth inhibition against SiHa cells (IC50 value = 9.60 μg/mL). Cytoselective effects were shown as no cytotoxicity observed in the treated normal NIH cells. SF1 induced early apoptosis event in SiHa cells as resulted by FITC-Annexin V/propidium iodide staining by flowcytometric analysis. The protein expression by flowcytometry using specific antibody conjugated fluorescent dye showed up-regulation of Bax and down-regulation Bcl-2 as well as increment of cytochrome C levels upon treatment with SF1. In conclusion, the findings suggested that SF1 demonstrated antiproliferative effects and induced apoptosis in SiHa cells through up-regulation of Bax and down-regulation of Bcl-2 via mitochondrial-dependent pathway. Penerbit Universiti Kebangsaan Malaysia 2019-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14354/1/21%20Nik%20Aina%20Syazana%20Nik%20Zainuddin.pdf Nik Aina Syazana Nik Zainuddin, and Hussin Muhammad, and Nik Fakhuruddin Nik Hassan, and Nor Hayati Othman, and Yusmazura Zakaria, (2019) Semi-purified fraction of Clinacanthus nutans induced apoptosis in human cervical cancer, SiHa cells via up-regulation of bax and down-regulation of Bcl-2. Sains Malaysiana, 48 (9). pp. 1997-2006. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil9_2019/KandunganJilid48Bil9_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Cervical cancer is a leading cause of death in women, worldwide, and second in Malaysia. Nowadays, modern cancer treatment comes with negative and adverse side effects to the patients. Traditional medicinal plants such as Clinacanthus nutans has been locally recognized for its medicinal properties and was claimed for cancer treatment. According to our pilot study, a fraction of C. nutans has shown a potent inhibition on human cervical cancer cells, SiHa, in vitro. Hence, this study aimed to investigate the underlying anticancer mechanism of a semi-purified fraction (SF1) of C. nutans towards cervical cancer cells. Bioassay guided fractionation and further purification was carried out. All collected fractions of C. nutans were examined for cytotoxicity activity towards HeLa, SiHa and NIH cells by MTT Assay. SF1 exhibited better cytotoxic activity with best growth inhibition against SiHa cells (IC50 value = 9.60 μg/mL). Cytoselective effects were shown as no cytotoxicity observed in the treated normal NIH cells. SF1 induced early apoptosis event in SiHa cells as resulted by FITC-Annexin V/propidium iodide staining by flowcytometric analysis. The protein expression by flowcytometry using specific antibody conjugated fluorescent dye showed up-regulation of Bax and down-regulation Bcl-2 as well as increment of cytochrome C levels upon treatment with SF1. In conclusion, the findings suggested that SF1 demonstrated antiproliferative effects and induced apoptosis in SiHa cells through up-regulation of Bax and down-regulation of Bcl-2 via mitochondrial-dependent pathway.
format	Article
author	Nik Aina Syazana Nik Zainuddin, Hussin Muhammad, Nik Fakhuruddin Nik Hassan, Nor Hayati Othman, Yusmazura Zakaria,
spellingShingle	Nik Aina Syazana Nik Zainuddin, Hussin Muhammad, Nik Fakhuruddin Nik Hassan, Nor Hayati Othman, Yusmazura Zakaria, Semi-purified fraction of Clinacanthus nutans induced apoptosis in human cervical cancer, SiHa cells via up-regulation of bax and down-regulation of Bcl-2
author_facet	Nik Aina Syazana Nik Zainuddin, Hussin Muhammad, Nik Fakhuruddin Nik Hassan, Nor Hayati Othman, Yusmazura Zakaria,
author_sort	Nik Aina Syazana Nik Zainuddin,
title	Semi-purified fraction of Clinacanthus nutans induced apoptosis in human cervical cancer, SiHa cells via up-regulation of bax and down-regulation of Bcl-2
title_short	Semi-purified fraction of Clinacanthus nutans induced apoptosis in human cervical cancer, SiHa cells via up-regulation of bax and down-regulation of Bcl-2
title_full	Semi-purified fraction of Clinacanthus nutans induced apoptosis in human cervical cancer, SiHa cells via up-regulation of bax and down-regulation of Bcl-2
title_fullStr	Semi-purified fraction of Clinacanthus nutans induced apoptosis in human cervical cancer, SiHa cells via up-regulation of bax and down-regulation of Bcl-2
title_full_unstemmed	Semi-purified fraction of Clinacanthus nutans induced apoptosis in human cervical cancer, SiHa cells via up-regulation of bax and down-regulation of Bcl-2
title_sort	semi-purified fraction of clinacanthus nutans induced apoptosis in human cervical cancer, siha cells via up-regulation of bax and down-regulation of bcl-2
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/14354/ http://journalarticle.ukm.my/14354/ http://journalarticle.ukm.my/14354/1/21%20Nik%20Aina%20Syazana%20Nik%20Zainuddin.pdf
first_indexed	2023-09-18T20:06:54Z
last_indexed	2023-09-18T20:06:54Z
_version_	1777407219369771008

Semi-purified fraction of Clinacanthus nutans induced apoptosis in human cervical cancer, SiHa cells via up-regulation of bax and down-regulation of Bcl-2

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