Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells

Hematopoietic stem cells (HSCs) transplantation was introduced as curative treatment for various diseases. Cryopreservation of HSCs is crucial for long term storage and maintenance of cellular quality; however, it has been reported that cryopreservation itself causes oxidative stress-driven apoptosi...

Full description

Bibliographic Details
Main Authors:	Shawal Maradona Abdul Wahab, Zariyantey Abd Hamid, Ramya Dewi Mathialagan, Izatus Shima Taib
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/14348/ http://journalarticle.ukm.my/14348/ http://journalarticle.ukm.my/14348/1/15%20Shawal%20Maradona%20Abdul%20Wahab.pdf

id	ukm-14348
recordtype	eprints
spelling	ukm-143482020-03-03T08:02:26Z http://journalarticle.ukm.my/14348/ Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells Shawal Maradona Abdul Wahab, Zariyantey Abd Hamid, Ramya Dewi Mathialagan, Izatus Shima Taib, Hematopoietic stem cells (HSCs) transplantation was introduced as curative treatment for various diseases. Cryopreservation of HSCs is crucial for long term storage and maintenance of cellular quality; however, it has been reported that cryopreservation itself causes oxidative stress-driven apoptosis and cell loss. This study investigated impact of supplementing N-acetylcysteine (NAC) as antioxidant during cryopreservation on viability and oxidative stress in HSCs. HSCs were isolated from murine bone marrow, cultured in HSCs growth media and cryopreserved (1×106 cells per vial) together with 10% DMSO and NAC (0, 0.25, 0.5 or 2.0 μM) for 48 h, 2 weeks or 8 weeks at -196°C using controlled-rate-freezing technique. Cell viability and oxidative stress in cryopreserved cells were analysed at each time-point. Cell viability was significantly reduced (p<0.05) following cryopreservation as compared to pre-cryopreservation. NAC supplementation significantly increased cell viability (p<0.05) after 48 h cryopreservation at 0.5 μM and 2.0 μM and after 2 weeks cryopreservation at 0.25 μM compared to the controls. Cryopreservation significantly enhanced GSH level (p<0.05) and reduced MDA level (p<0.05) without affecting SOD activity and PC level in HSCs compared to pre-cryopreservation. NAC supplementation significantly increased GSH level at 0.25 μM in cryopreserved HSCs compared to control. In conclusion, NAC supplementation during cryopreservation showed potential in minimizing cryoinjury by promoting cell viability, increasing antioxidant capacity and reducing oxidative damage in HSCs, however these effects are influenced by both durations of cryopreservation and NAC concentration. Penerbit Universiti Kebangsaan Malaysia 2019-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14348/1/15%20Shawal%20Maradona%20Abdul%20Wahab.pdf Shawal Maradona Abdul Wahab, and Zariyantey Abd Hamid, and Ramya Dewi Mathialagan, and Izatus Shima Taib, (2019) Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells. Sains Malaysiana, 48 (9). pp. 1937-1946. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil9_2019/KandunganJilid48Bil9_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Hematopoietic stem cells (HSCs) transplantation was introduced as curative treatment for various diseases. Cryopreservation of HSCs is crucial for long term storage and maintenance of cellular quality; however, it has been reported that cryopreservation itself causes oxidative stress-driven apoptosis and cell loss. This study investigated impact of supplementing N-acetylcysteine (NAC) as antioxidant during cryopreservation on viability and oxidative stress in HSCs. HSCs were isolated from murine bone marrow, cultured in HSCs growth media and cryopreserved (1×106 cells per vial) together with 10% DMSO and NAC (0, 0.25, 0.5 or 2.0 μM) for 48 h, 2 weeks or 8 weeks at -196°C using controlled-rate-freezing technique. Cell viability and oxidative stress in cryopreserved cells were analysed at each time-point. Cell viability was significantly reduced (p<0.05) following cryopreservation as compared to pre-cryopreservation. NAC supplementation significantly increased cell viability (p<0.05) after 48 h cryopreservation at 0.5 μM and 2.0 μM and after 2 weeks cryopreservation at 0.25 μM compared to the controls. Cryopreservation significantly enhanced GSH level (p<0.05) and reduced MDA level (p<0.05) without affecting SOD activity and PC level in HSCs compared to pre-cryopreservation. NAC supplementation significantly increased GSH level at 0.25 μM in cryopreserved HSCs compared to control. In conclusion, NAC supplementation during cryopreservation showed potential in minimizing cryoinjury by promoting cell viability, increasing antioxidant capacity and reducing oxidative damage in HSCs, however these effects are influenced by both durations of cryopreservation and NAC concentration.
format	Article
author	Shawal Maradona Abdul Wahab, Zariyantey Abd Hamid, Ramya Dewi Mathialagan, Izatus Shima Taib,
spellingShingle	Shawal Maradona Abdul Wahab, Zariyantey Abd Hamid, Ramya Dewi Mathialagan, Izatus Shima Taib, Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
author_facet	Shawal Maradona Abdul Wahab, Zariyantey Abd Hamid, Ramya Dewi Mathialagan, Izatus Shima Taib,
author_sort	Shawal Maradona Abdul Wahab,
title	Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
title_short	Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
title_full	Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
title_fullStr	Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
title_full_unstemmed	Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
title_sort	effect of n-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/14348/ http://journalarticle.ukm.my/14348/ http://journalarticle.ukm.my/14348/1/15%20Shawal%20Maradona%20Abdul%20Wahab.pdf
first_indexed	2023-09-18T20:06:54Z
last_indexed	2023-09-18T20:06:54Z
_version_	1777407218505744384

Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells

Similar Items