Inhibitory effects of Gynura procumbens ethanolic extract on nitric oxide production and inducible nitric oxide synthase (iNOS) protein expression in macrophages

Nitric oxide (NO) overproduction by inducible nitric oxide synthase (iNOS) may be associated with acute and chronic inflammations. Macrophages as important cells in the innate immune system are able to be stimulated and can lead to iNOS activation and excessive NO production. Gynura procumbens is a...

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Main Authors:	Tan, Jiah Ning, Syaratul Dalina Yusoff, Zakiah Jubri, Fhataheya Buang, Tan, Ze Song, Ameerah Budiono, Ibrahim Jantan, Roza Dianita, Endang Kumolosasi, Norazrina Azmi, Norsyahida Mohd Fauzi
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13897/ http://journalarticle.ukm.my/13897/ http://journalarticle.ukm.my/13897/1/20%20Tan%20Jiah%20Ning.pdf

id	ukm-13897
recordtype	eprints
spelling	ukm-138972020-01-10T08:51:14Z http://journalarticle.ukm.my/13897/ Inhibitory effects of Gynura procumbens ethanolic extract on nitric oxide production and inducible nitric oxide synthase (iNOS) protein expression in macrophages Tan, Jiah Ning Syaratul Dalina Yusoff, Zakiah Jubri, Fhataheya Buang, Tan, Ze Song Ameerah Budiono, Ibrahim Jantan, Roza Dianita, Endang Kumolosasi, Norazrina Azmi, Norsyahida Mohd Fauzi, Nitric oxide (NO) overproduction by inducible nitric oxide synthase (iNOS) may be associated with acute and chronic inflammations. Macrophages as important cells in the innate immune system are able to be stimulated and can lead to iNOS activation and excessive NO production. Gynura procumbens is a medicinal plant traditionally used in treating various ailments including inflammation but the mechanism of anti-inflammatory activity of this plant is still elusive. This study was carried out to investigate the anti-inflammatory therapeutic effects of Gynura procumbens ethanolic extract on NO production and iNOS protein expression in RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS). Cell viability of RAW 264.7 macrophages treated with Gynura procumbens ethanolic extract was determined by MTT assay. NO production was determined by Griess assay following Gynura procumbens ethanolic extract treatment alone or in combination with LPS stimulation. Protein expression of iNOS was determined by western blot. RAW 264.7 macrophages viability of more than 90% was observed after 24 h treatment with Gynura procumbens ethanolic extract concentration range of 3.9 μg/mL to 500 μg/mL. Significant inhibition of NO production level has been identified in LPS-stimulated RAW 264.7 cells pre-treated with 250 μg/mL Gynura procumbens ethanolic extract (p<0.05) while all selected concentrations of Gynura procumbens ethanolic extract showed no significant alteration of NO production in the absence of LPS stimulation. Pre-treatment of 250 μg/mL Gynura procumbens ethanolic extract also demonstrated significant suppression of iNOS protein expression in LPS-stimulated RAW 264.7 cells (p<0.05). In conclusion, this study demonstrates that Gynura procumbens ethanolic extract exhibits anti-inflammatory potential through inhibition of NO production and iNOS protein expression in LPS-stimulated macrophages, suggesting that this plant could be further researched for its beneficial use in inflammatory disorders. Penerbit Universiti Kebangsaan Malaysia 2019-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13897/1/20%20Tan%20Jiah%20Ning.pdf Tan, Jiah Ning and Syaratul Dalina Yusoff, and Zakiah Jubri, and Fhataheya Buang, and Tan, Ze Song and Ameerah Budiono, and Ibrahim Jantan, and Roza Dianita, and Endang Kumolosasi, and Norazrina Azmi, and Norsyahida Mohd Fauzi, (2019) Inhibitory effects of Gynura procumbens ethanolic extract on nitric oxide production and inducible nitric oxide synthase (iNOS) protein expression in macrophages. Sains Malaysiana, 48 (8). pp. 1737-1744. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil8_2019/KandunganJilid48Bil8_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Nitric oxide (NO) overproduction by inducible nitric oxide synthase (iNOS) may be associated with acute and chronic inflammations. Macrophages as important cells in the innate immune system are able to be stimulated and can lead to iNOS activation and excessive NO production. Gynura procumbens is a medicinal plant traditionally used in treating various ailments including inflammation but the mechanism of anti-inflammatory activity of this plant is still elusive. This study was carried out to investigate the anti-inflammatory therapeutic effects of Gynura procumbens ethanolic extract on NO production and iNOS protein expression in RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS). Cell viability of RAW 264.7 macrophages treated with Gynura procumbens ethanolic extract was determined by MTT assay. NO production was determined by Griess assay following Gynura procumbens ethanolic extract treatment alone or in combination with LPS stimulation. Protein expression of iNOS was determined by western blot. RAW 264.7 macrophages viability of more than 90% was observed after 24 h treatment with Gynura procumbens ethanolic extract concentration range of 3.9 μg/mL to 500 μg/mL. Significant inhibition of NO production level has been identified in LPS-stimulated RAW 264.7 cells pre-treated with 250 μg/mL Gynura procumbens ethanolic extract (p<0.05) while all selected concentrations of Gynura procumbens ethanolic extract showed no significant alteration of NO production in the absence of LPS stimulation. Pre-treatment of 250 μg/mL Gynura procumbens ethanolic extract also demonstrated significant suppression of iNOS protein expression in LPS-stimulated RAW 264.7 cells (p<0.05). In conclusion, this study demonstrates that Gynura procumbens ethanolic extract exhibits anti-inflammatory potential through inhibition of NO production and iNOS protein expression in LPS-stimulated macrophages, suggesting that this plant could be further researched for its beneficial use in inflammatory disorders.
format	Article
author	Tan, Jiah Ning Syaratul Dalina Yusoff, Zakiah Jubri, Fhataheya Buang, Tan, Ze Song Ameerah Budiono, Ibrahim Jantan, Roza Dianita, Endang Kumolosasi, Norazrina Azmi, Norsyahida Mohd Fauzi,
spellingShingle	Tan, Jiah Ning Syaratul Dalina Yusoff, Zakiah Jubri, Fhataheya Buang, Tan, Ze Song Ameerah Budiono, Ibrahim Jantan, Roza Dianita, Endang Kumolosasi, Norazrina Azmi, Norsyahida Mohd Fauzi, Inhibitory effects of Gynura procumbens ethanolic extract on nitric oxide production and inducible nitric oxide synthase (iNOS) protein expression in macrophages
author_facet	Tan, Jiah Ning Syaratul Dalina Yusoff, Zakiah Jubri, Fhataheya Buang, Tan, Ze Song Ameerah Budiono, Ibrahim Jantan, Roza Dianita, Endang Kumolosasi, Norazrina Azmi, Norsyahida Mohd Fauzi,
author_sort	Tan, Jiah Ning
title	Inhibitory effects of Gynura procumbens ethanolic extract on nitric oxide production and inducible nitric oxide synthase (iNOS) protein expression in macrophages
title_short	Inhibitory effects of Gynura procumbens ethanolic extract on nitric oxide production and inducible nitric oxide synthase (iNOS) protein expression in macrophages
title_full	Inhibitory effects of Gynura procumbens ethanolic extract on nitric oxide production and inducible nitric oxide synthase (iNOS) protein expression in macrophages
title_fullStr	Inhibitory effects of Gynura procumbens ethanolic extract on nitric oxide production and inducible nitric oxide synthase (iNOS) protein expression in macrophages
title_full_unstemmed	Inhibitory effects of Gynura procumbens ethanolic extract on nitric oxide production and inducible nitric oxide synthase (iNOS) protein expression in macrophages
title_sort	inhibitory effects of gynura procumbens ethanolic extract on nitric oxide production and inducible nitric oxide synthase (inos) protein expression in macrophages
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13897/ http://journalarticle.ukm.my/13897/ http://journalarticle.ukm.my/13897/1/20%20Tan%20Jiah%20Ning.pdf
first_indexed	2023-09-18T20:05:52Z
last_indexed	2023-09-18T20:05:52Z
_version_	1777407154468159488

Inhibitory effects of Gynura procumbens ethanolic extract on nitric oxide production and inducible nitric oxide synthase (iNOS) protein expression in macrophages

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