Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia

Coccidiosis, caused by the Eimeria species, greatly affects the poultry industry. Severity of the disease varies depending on the identity of the infecting parasites, encouraging identification of Eimeria species circulating on a farm as a valuable component of chicken management. Conventional metho...

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Main Authors:	Loo, Shu-San, Lim, Lik-Sin, Nurul-Ain Efendi, Blake, Damer P., Kawazu, Shin-ichiro, Wan, Kiew-Lian
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13736/ http://journalarticle.ukm.my/13736/ http://journalarticle.ukm.my/13736/1/11%20Shu-San%20Loo.pdf

id	ukm-13736
recordtype	eprints
spelling	ukm-137362019-11-29T09:18:06Z http://journalarticle.ukm.my/13736/ Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia Loo, Shu-San Lim, Lik-Sin Nurul-Ain Efendi, Blake, Damer P. Kawazu, Shin-ichiro Wan, Kiew-Lian Coccidiosis, caused by the Eimeria species, greatly affects the poultry industry. Severity of the disease varies depending on the identity of the infecting parasites, encouraging identification of Eimeria species circulating on a farm as a valuable component of chicken management. Conventional methods of Eimeria species identification are time consuming and can be subjective in nature. Given these limitations, molecular approaches have been developed for specific detection of Eimeria species. In this study, faecal samples were collected from commercial broiler farms and subjected to microscopic examination for Eimeria occurrence. Eimeria species were putatively identified by morphological characterisation and grouped into three categories based on oocyst size. Molecular detection of Eimeria species occurrence in these samples was then performed using two published PCR assays (the individual components of a SCAR-based multiplex PCR, and assays developed for quantitative PCR, termed PCR-SCAR 1 and PCR-SCAR 2 here) and a LAMP assay. Comparison of the results obtained demonstrated that the three molecular methods were capable of detecting all Eimeria species of the reference Houghton strain, but showed varying efficiencies in detecting Malaysian field isolates. PCR-SCAR 2 was found to be the most effective, detecting all seven Eimeria species and indicating the presence of Eimeria parasites in most flocks. Differences in the ability of the molecular methods to detect Eimeria may be a consequence of sequence divergence between isolates from different regions, implying that development of region-specific methods using local Eimeria strains may be required to improve the efficiency of molecular assays for Eimeria detection. Penerbit Universiti Kebangsaan Malaysia 2019-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13736/1/11%20Shu-San%20Loo.pdf Loo, Shu-San and Lim, Lik-Sin and Nurul-Ain Efendi, and Blake, Damer P. and Kawazu, Shin-ichiro and Wan, Kiew-Lian (2019) Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia. Sains Malaysiana, 48 (7). pp. 1425-1432. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil7_2019/KandunganJilid48Bil7_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Coccidiosis, caused by the Eimeria species, greatly affects the poultry industry. Severity of the disease varies depending on the identity of the infecting parasites, encouraging identification of Eimeria species circulating on a farm as a valuable component of chicken management. Conventional methods of Eimeria species identification are time consuming and can be subjective in nature. Given these limitations, molecular approaches have been developed for specific detection of Eimeria species. In this study, faecal samples were collected from commercial broiler farms and subjected to microscopic examination for Eimeria occurrence. Eimeria species were putatively identified by morphological characterisation and grouped into three categories based on oocyst size. Molecular detection of Eimeria species occurrence in these samples was then performed using two published PCR assays (the individual components of a SCAR-based multiplex PCR, and assays developed for quantitative PCR, termed PCR-SCAR 1 and PCR-SCAR 2 here) and a LAMP assay. Comparison of the results obtained demonstrated that the three molecular methods were capable of detecting all Eimeria species of the reference Houghton strain, but showed varying efficiencies in detecting Malaysian field isolates. PCR-SCAR 2 was found to be the most effective, detecting all seven Eimeria species and indicating the presence of Eimeria parasites in most flocks. Differences in the ability of the molecular methods to detect Eimeria may be a consequence of sequence divergence between isolates from different regions, implying that development of region-specific methods using local Eimeria strains may be required to improve the efficiency of molecular assays for Eimeria detection.
format	Article
author	Loo, Shu-San Lim, Lik-Sin Nurul-Ain Efendi, Blake, Damer P. Kawazu, Shin-ichiro Wan, Kiew-Lian
spellingShingle	Loo, Shu-San Lim, Lik-Sin Nurul-Ain Efendi, Blake, Damer P. Kawazu, Shin-ichiro Wan, Kiew-Lian Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia
author_facet	Loo, Shu-San Lim, Lik-Sin Nurul-Ain Efendi, Blake, Damer P. Kawazu, Shin-ichiro Wan, Kiew-Lian
author_sort	Loo, Shu-San
title	Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia
title_short	Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia
title_full	Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia
title_fullStr	Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia
title_full_unstemmed	Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia
title_sort	comparison of molecular methods for the detection of eimeria in domestic chickens in malaysia
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13736/ http://journalarticle.ukm.my/13736/ http://journalarticle.ukm.my/13736/1/11%20Shu-San%20Loo.pdf
first_indexed	2023-09-18T20:05:31Z
last_indexed	2023-09-18T20:05:31Z
_version_	1777407132087353344

Comparison of molecular methods for the detection of Eimeria in domestic chickens in Malaysia

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