Comparison of phenolic constituent in Hibiscus sabdariffa cv. UKMR-2 calyx at different harvesting times

The metabolic changes in the phenolics content of Hibiscus sabdariffa cv. UKMR-2 were investigated at different harvesting times based on the High-Performance Liquid Chromatographic method using a photodiode array detector (HPLC-PDA) with gradient elution. The antioxidant activity was also determine...

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Main Authors:	Siti Aishah Mohd Ali, Che Radziah Che Mohd Zain, Jalifah Latip
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13735/ http://journalarticle.ukm.my/13735/ http://journalarticle.ukm.my/13735/1/10%20Siti%20Aishah%20Mohd%20Ali.pdf

id	ukm-13735
recordtype	eprints
spelling	ukm-137352019-11-29T09:17:36Z http://journalarticle.ukm.my/13735/ Comparison of phenolic constituent in Hibiscus sabdariffa cv. UKMR-2 calyx at different harvesting times Siti Aishah Mohd Ali, Che Radziah Che Mohd Zain, Jalifah Latip, The metabolic changes in the phenolics content of Hibiscus sabdariffa cv. UKMR-2 were investigated at different harvesting times based on the High-Performance Liquid Chromatographic method using a photodiode array detector (HPLC-PDA) with gradient elution. The antioxidant activity was also determined using 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity (DPPH) assay. The cultivation was executed under a control condition, and the calyces were separately harvested at four maturation stages. UKMR-2 calyces were extracted with water via sonication (50°C, 30 min). HPLC-PDA analysis showed two predominant anthocyanins, namely delphinidin-3-O-sambubioside (2.07-2.47 mg/g DW) and cyanidin-3-O-sambubioside (0.55-1.01 mg/g DW). In addition, ascorbic acid (3.34-9.88 mg/g DW), caffeic acid (0.08-0.09 mg/g DW) and chlorogenic acid (0.50-0.65 mg/g DW) were also detected in all maturity stages. Cyanidin-3-O-sambubioside content increased as the calyx became more mature, whereas the delphinidin-3-O-sambubioside, ascorbic acid, and chlorogenic acid content declined towards calyx maturity. The antioxidant activity gradually increased as the calyx ripening progressed. However, the activities did not differ significantly between the stages (p>0.05). The high content of total phenolic, total anthocyanins, and free radical scavenging activity were detected at Stage 4 (28-30 DAA) in UKMR-2, suggesting that this stage is the most appropriate maturity stage for harvesting H. sabdariffa cv. UKMR-2 calyces compared to the other maturity stages. Penerbit Universiti Kebangsaan Malaysia 2019-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13735/1/10%20Siti%20Aishah%20Mohd%20Ali.pdf Siti Aishah Mohd Ali, and Che Radziah Che Mohd Zain, and Jalifah Latip, (2019) Comparison of phenolic constituent in Hibiscus sabdariffa cv. UKMR-2 calyx at different harvesting times. Sains Malaysiana, 48 (7). pp. 1417-1424. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil7_2019/KandunganJilid48Bil7_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The metabolic changes in the phenolics content of Hibiscus sabdariffa cv. UKMR-2 were investigated at different harvesting times based on the High-Performance Liquid Chromatographic method using a photodiode array detector (HPLC-PDA) with gradient elution. The antioxidant activity was also determined using 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity (DPPH) assay. The cultivation was executed under a control condition, and the calyces were separately harvested at four maturation stages. UKMR-2 calyces were extracted with water via sonication (50°C, 30 min). HPLC-PDA analysis showed two predominant anthocyanins, namely delphinidin-3-O-sambubioside (2.07-2.47 mg/g DW) and cyanidin-3-O-sambubioside (0.55-1.01 mg/g DW). In addition, ascorbic acid (3.34-9.88 mg/g DW), caffeic acid (0.08-0.09 mg/g DW) and chlorogenic acid (0.50-0.65 mg/g DW) were also detected in all maturity stages. Cyanidin-3-O-sambubioside content increased as the calyx became more mature, whereas the delphinidin-3-O-sambubioside, ascorbic acid, and chlorogenic acid content declined towards calyx maturity. The antioxidant activity gradually increased as the calyx ripening progressed. However, the activities did not differ significantly between the stages (p>0.05). The high content of total phenolic, total anthocyanins, and free radical scavenging activity were detected at Stage 4 (28-30 DAA) in UKMR-2, suggesting that this stage is the most appropriate maturity stage for harvesting H. sabdariffa cv. UKMR-2 calyces compared to the other maturity stages.
format	Article
author	Siti Aishah Mohd Ali, Che Radziah Che Mohd Zain, Jalifah Latip,
spellingShingle	Siti Aishah Mohd Ali, Che Radziah Che Mohd Zain, Jalifah Latip, Comparison of phenolic constituent in Hibiscus sabdariffa cv. UKMR-2 calyx at different harvesting times
author_facet	Siti Aishah Mohd Ali, Che Radziah Che Mohd Zain, Jalifah Latip,
author_sort	Siti Aishah Mohd Ali,
title	Comparison of phenolic constituent in Hibiscus sabdariffa cv. UKMR-2 calyx at different harvesting times
title_short	Comparison of phenolic constituent in Hibiscus sabdariffa cv. UKMR-2 calyx at different harvesting times
title_full	Comparison of phenolic constituent in Hibiscus sabdariffa cv. UKMR-2 calyx at different harvesting times
title_fullStr	Comparison of phenolic constituent in Hibiscus sabdariffa cv. UKMR-2 calyx at different harvesting times
title_full_unstemmed	Comparison of phenolic constituent in Hibiscus sabdariffa cv. UKMR-2 calyx at different harvesting times
title_sort	comparison of phenolic constituent in hibiscus sabdariffa cv. ukmr-2 calyx at different harvesting times
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13735/ http://journalarticle.ukm.my/13735/ http://journalarticle.ukm.my/13735/1/10%20Siti%20Aishah%20Mohd%20Ali.pdf
first_indexed	2023-09-18T20:05:31Z
last_indexed	2023-09-18T20:05:31Z
_version_	1777407131941601280

Comparison of phenolic constituent in Hibiscus sabdariffa cv. UKMR-2 calyx at different harvesting times

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