A hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres

The sensitive and rapid detection of hydrogen peroxide is very important in the areas of clinical and environmental analyses. A sensitive and selective Horseradish peroxidase (HRP)-hydrogen peroxide (H2O2) biosensor was developed based on acrylic microspheres. Hydrophobic poly(n-butyl acrylate-N-acr...

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Main Authors: Eda Yuhana Ariffin, Nik Nurhanan Nik Mansor, Safitri, Eka, Lee, Yook Heng, Nurul Izzaty Hassan
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/13734/
http://journalarticle.ukm.my/13734/
http://journalarticle.ukm.my/13734/1/09%20Eda%20Yuhana%20Ariffin.pdf
id ukm-13734
recordtype eprints
spelling ukm-137342019-11-29T09:17:00Z http://journalarticle.ukm.my/13734/ A hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres Eda Yuhana Ariffin, Nik Nurhanan Nik Mansor, Safitri, Eka Lee, Yook Heng Nurul Izzaty Hassan, The sensitive and rapid detection of hydrogen peroxide is very important in the areas of clinical and environmental analyses. A sensitive and selective Horseradish peroxidase (HRP)-hydrogen peroxide (H2O2) biosensor was developed based on acrylic microspheres. Hydrophobic poly(n-butyl acrylate-N-acryloxysuccinimide) [poly(nBA-NAS)] microspheres were synthesized using photopolymerization in an emulsion to form an enzyme immobilization matrix. The HRP enzyme was covalently immobilized onto the acrylic microspheres via the succinimide functionality. Field emission scanning electron microscope (FESEM) has been utilized to characterize the screen-printed carbon paste electrode (SPE) constructed from enzyme conjugated acrylic microspheres and gold nanoparticles (AuNPs) composite (HRP/nBA-NAS/AuNPs/SPE). Differential pulse voltammetry was used to assess the biosensor performance. The linear response range of the hydrogen peroxide biosensor obtained was from 1.0 × 10-2 to 1.0 × 10-10 M (R2 = 0.99) with the limit of detection (LOD) approximately at 1.0 × 10-10 M. This is an improvement over many hydrogen peroxide biosensors reported so far. Such improvement may be attributed to the large surface area provided by the acrylic microspheres as a matrix for immobilization of the HRP enzyme. Penerbit Universiti Kebangsaan Malaysia 2019-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13734/1/09%20Eda%20Yuhana%20Ariffin.pdf Eda Yuhana Ariffin, and Nik Nurhanan Nik Mansor, and Safitri, Eka and Lee, Yook Heng and Nurul Izzaty Hassan, (2019) A hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres. Sains Malaysiana, 48 (7). pp. 1409-1416. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil7_2019/KandunganJilid48Bil7_2019.html
repository_type Digital Repository
institution_category Local University
institution Universiti Kebangasaan Malaysia
building UKM Institutional Repository
collection Online Access
language English
description The sensitive and rapid detection of hydrogen peroxide is very important in the areas of clinical and environmental analyses. A sensitive and selective Horseradish peroxidase (HRP)-hydrogen peroxide (H2O2) biosensor was developed based on acrylic microspheres. Hydrophobic poly(n-butyl acrylate-N-acryloxysuccinimide) [poly(nBA-NAS)] microspheres were synthesized using photopolymerization in an emulsion to form an enzyme immobilization matrix. The HRP enzyme was covalently immobilized onto the acrylic microspheres via the succinimide functionality. Field emission scanning electron microscope (FESEM) has been utilized to characterize the screen-printed carbon paste electrode (SPE) constructed from enzyme conjugated acrylic microspheres and gold nanoparticles (AuNPs) composite (HRP/nBA-NAS/AuNPs/SPE). Differential pulse voltammetry was used to assess the biosensor performance. The linear response range of the hydrogen peroxide biosensor obtained was from 1.0 × 10-2 to 1.0 × 10-10 M (R2 = 0.99) with the limit of detection (LOD) approximately at 1.0 × 10-10 M. This is an improvement over many hydrogen peroxide biosensors reported so far. Such improvement may be attributed to the large surface area provided by the acrylic microspheres as a matrix for immobilization of the HRP enzyme.
format Article
author Eda Yuhana Ariffin,
Nik Nurhanan Nik Mansor,
Safitri, Eka
Lee, Yook Heng
Nurul Izzaty Hassan,
spellingShingle Eda Yuhana Ariffin,
Nik Nurhanan Nik Mansor,
Safitri, Eka
Lee, Yook Heng
Nurul Izzaty Hassan,
A hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres
author_facet Eda Yuhana Ariffin,
Nik Nurhanan Nik Mansor,
Safitri, Eka
Lee, Yook Heng
Nurul Izzaty Hassan,
author_sort Eda Yuhana Ariffin,
title A hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres
title_short A hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres
title_full A hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres
title_fullStr A hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres
title_full_unstemmed A hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres
title_sort hydrogen peroxide biosensor from horseradish peroxidase immobilization onto acrylic microspheres
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/13734/
http://journalarticle.ukm.my/13734/
http://journalarticle.ukm.my/13734/1/09%20Eda%20Yuhana%20Ariffin.pdf
first_indexed 2023-09-18T20:05:31Z
last_indexed 2023-09-18T20:05:31Z
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