High sensitivity au-based Kretschmann Surface Plasmon Resonance sensor for urea detection

Kretschmann-based Surface Plasmon Resonance (SPR) optical sensor was applied to detect the presence of kidney wastes such as urea in solutions. To enhance the sensitivity of the SPR sensor, nanolaminated gold film (thickness of 50 nm) was used. In this work, the SPR response to urea in various conce...

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Main Authors:	P. Susthitha Menon, Fairus Atida Said, Gan, Siew Mei, Mohd Ambri Mohamed, Ahmad Rifqi Md Zain, Sahbudin Shaari, Burhanuddin Yeop Majlis
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13709/ http://journalarticle.ukm.my/13709/ http://journalarticle.ukm.my/13709/1/04%20P.%20Susthitha%20Menon.pdf

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recordtype	eprints
spelling	ukm-137092019-11-29T08:56:28Z http://journalarticle.ukm.my/13709/ High sensitivity au-based Kretschmann Surface Plasmon Resonance sensor for urea detection P. Susthitha Menon, Fairus Atida Said, Gan, Siew Mei Mohd Ambri Mohamed, Ahmad Rifqi Md Zain, Sahbudin Shaari, Burhanuddin Yeop Majlis, Kretschmann-based Surface Plasmon Resonance (SPR) optical sensor was applied to detect the presence of kidney wastes such as urea in solutions. To enhance the sensitivity of the SPR sensor, nanolaminated gold film (thickness of 50 nm) was used. In this work, the SPR response to urea in various concentrations were measured and investigated using optical wavelengths of 670 nm and 785 nm. The signals were compared between pure urea solution versus mixed solution in the presence of the 0.227 μmol urease enzyme. The proposed mixed solution is to eliminate doping and gel entrapment process for enzyme immobilization in conventional method. Angular interrogation technique was used to measure the sensor performance in urea detection using pure and mixed solutions with urea concentration of 0 - 800 mM. Upon exposure to mixed solution using 785 nm optical wavelength, the nanolaminated gold film exhibited higher SPR sensitivity as much as 7.8 M-1 than a pure urea solution (1.4 M-1). The coupling activity between urea and urease molecules in the mixed solution near the nanolaminated gold film surface lead to sensitivity enhancement. Angle shifting of mixed solution on 50 nm-thick nanolaminated gold film using 670 nm optical wavelength was greater by ~50% compared to 785 nm. Sensorgram data shows a steady and linear increment in SPR incident angle shifting when urea concentration increased. To the best of our knowledge, this is the first time that Kretschmann-based SPR has been used for urea sensing at 670 nm and 785 nm optical wavelengths. Penerbit Universiti Kebangsaan Malaysia 2019-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13709/1/04%20P.%20Susthitha%20Menon.pdf P. Susthitha Menon, and Fairus Atida Said, and Gan, Siew Mei and Mohd Ambri Mohamed, and Ahmad Rifqi Md Zain, and Sahbudin Shaari, and Burhanuddin Yeop Majlis, (2019) High sensitivity au-based Kretschmann Surface Plasmon Resonance sensor for urea detection. Sains Malaysiana, 48 (6). pp. 1179-1185. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil6_2019/KandunganJilid48Bil6_2019.html
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institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Kretschmann-based Surface Plasmon Resonance (SPR) optical sensor was applied to detect the presence of kidney wastes such as urea in solutions. To enhance the sensitivity of the SPR sensor, nanolaminated gold film (thickness of 50 nm) was used. In this work, the SPR response to urea in various concentrations were measured and investigated using optical wavelengths of 670 nm and 785 nm. The signals were compared between pure urea solution versus mixed solution in the presence of the 0.227 μmol urease enzyme. The proposed mixed solution is to eliminate doping and gel entrapment process for enzyme immobilization in conventional method. Angular interrogation technique was used to measure the sensor performance in urea detection using pure and mixed solutions with urea concentration of 0 - 800 mM. Upon exposure to mixed solution using 785 nm optical wavelength, the nanolaminated gold film exhibited higher SPR sensitivity as much as 7.8 M-1 than a pure urea solution (1.4 M-1). The coupling activity between urea and urease molecules in the mixed solution near the nanolaminated gold film surface lead to sensitivity enhancement. Angle shifting of mixed solution on 50 nm-thick nanolaminated gold film using 670 nm optical wavelength was greater by ~50% compared to 785 nm. Sensorgram data shows a steady and linear increment in SPR incident angle shifting when urea concentration increased. To the best of our knowledge, this is the first time that Kretschmann-based SPR has been used for urea sensing at 670 nm and 785 nm optical wavelengths.
format	Article
author	P. Susthitha Menon, Fairus Atida Said, Gan, Siew Mei Mohd Ambri Mohamed, Ahmad Rifqi Md Zain, Sahbudin Shaari, Burhanuddin Yeop Majlis,
spellingShingle	P. Susthitha Menon, Fairus Atida Said, Gan, Siew Mei Mohd Ambri Mohamed, Ahmad Rifqi Md Zain, Sahbudin Shaari, Burhanuddin Yeop Majlis, High sensitivity au-based Kretschmann Surface Plasmon Resonance sensor for urea detection
author_facet	P. Susthitha Menon, Fairus Atida Said, Gan, Siew Mei Mohd Ambri Mohamed, Ahmad Rifqi Md Zain, Sahbudin Shaari, Burhanuddin Yeop Majlis,
author_sort	P. Susthitha Menon,
title	High sensitivity au-based Kretschmann Surface Plasmon Resonance sensor for urea detection
title_short	High sensitivity au-based Kretschmann Surface Plasmon Resonance sensor for urea detection
title_full	High sensitivity au-based Kretschmann Surface Plasmon Resonance sensor for urea detection
title_fullStr	High sensitivity au-based Kretschmann Surface Plasmon Resonance sensor for urea detection
title_full_unstemmed	High sensitivity au-based Kretschmann Surface Plasmon Resonance sensor for urea detection
title_sort	high sensitivity au-based kretschmann surface plasmon resonance sensor for urea detection
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13709/ http://journalarticle.ukm.my/13709/ http://journalarticle.ukm.my/13709/1/04%20P.%20Susthitha%20Menon.pdf
first_indexed	2023-09-18T20:05:28Z
last_indexed	2023-09-18T20:05:28Z
_version_	1777407128282071040

High sensitivity au-based Kretschmann Surface Plasmon Resonance sensor for urea detection

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