Optimization of cryopreserved single cell suspension of gastric epithelial cells for successful DotScanTM antibody microarray

Cell cryopreservation plays a pivotal role for successful immunological assays. DotScanTM antibody microarray employs live cells with satisfactory cell viability for detection of clusters of differentiation (CD) antigens in various diseases. Effective cryopreservation contributes to successful captu...

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Main Authors:	Asif Sukri, Alfizah Hanafiah, Nik Ritza Kosai Nik Mahmood, Mustafa Mohamed Taher, Isa Mohamed Rose, Ramelah Mohamed
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:	http://journalarticle.ukm.my/13632/ http://journalarticle.ukm.my/13632/ http://journalarticle.ukm.my/13632/1/19%20Asif%20Sukri.pdf

id	ukm-13632
recordtype	eprints
spelling	ukm-136322019-11-10T03:30:39Z http://journalarticle.ukm.my/13632/ Optimization of cryopreserved single cell suspension of gastric epithelial cells for successful DotScanTM antibody microarray Asif Sukri, Alfizah Hanafiah, Nik Ritza Kosai Nik Mahmood, Mustafa Mohamed Taher, Isa Mohamed Rose, Ramelah Mohamed, Cell cryopreservation plays a pivotal role for successful immunological assays. DotScanTM antibody microarray employs live cells with satisfactory cell viability for detection of clusters of differentiation (CD) antigens in various diseases. Effective cryopreservation contributes to successful capture of cells on DotScanTM antibody microarray. The purpose of this study was to evaluate the effect of cells viability on cells binding to specific CD antigens. Cells with similar concentration but different percentage of cell viabilities were captured on DotScanTM slides. Cells stored for 5 days at -80ºC and added with freezing medium in stepwise manner had higher viabilities as compared to cells stored for 6 months at -80ºC and added with freezing medium in direct manner (p=0.012). Cells with 88% viability had higher binding to CD44/CD29 control antibodies as compared to cells with 33% viability. Short-term storage of cells and the technique for freezing medium addition to cells play a critical role for successful cryopreservation of single cell suspension. Penerbit Universiti Kebangsaan Malaysia 2019-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13632/1/19%20Asif%20Sukri.pdf Asif Sukri, and Alfizah Hanafiah, and Nik Ritza Kosai Nik Mahmood, and Mustafa Mohamed Taher, and Isa Mohamed Rose, and Ramelah Mohamed, (2019) Optimization of cryopreserved single cell suspension of gastric epithelial cells for successful DotScanTM antibody microarray. Sains Malaysiana, 48 (5). pp. 1105-1110. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil5_2019/KandunganJilid48Bil5_2019.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Cell cryopreservation plays a pivotal role for successful immunological assays. DotScanTM antibody microarray employs live cells with satisfactory cell viability for detection of clusters of differentiation (CD) antigens in various diseases. Effective cryopreservation contributes to successful capture of cells on DotScanTM antibody microarray. The purpose of this study was to evaluate the effect of cells viability on cells binding to specific CD antigens. Cells with similar concentration but different percentage of cell viabilities were captured on DotScanTM slides. Cells stored for 5 days at -80ºC and added with freezing medium in stepwise manner had higher viabilities as compared to cells stored for 6 months at -80ºC and added with freezing medium in direct manner (p=0.012). Cells with 88% viability had higher binding to CD44/CD29 control antibodies as compared to cells with 33% viability. Short-term storage of cells and the technique for freezing medium addition to cells play a critical role for successful cryopreservation of single cell suspension.
format	Article
author	Asif Sukri, Alfizah Hanafiah, Nik Ritza Kosai Nik Mahmood, Mustafa Mohamed Taher, Isa Mohamed Rose, Ramelah Mohamed,
spellingShingle	Asif Sukri, Alfizah Hanafiah, Nik Ritza Kosai Nik Mahmood, Mustafa Mohamed Taher, Isa Mohamed Rose, Ramelah Mohamed, Optimization of cryopreserved single cell suspension of gastric epithelial cells for successful DotScanTM antibody microarray
author_facet	Asif Sukri, Alfizah Hanafiah, Nik Ritza Kosai Nik Mahmood, Mustafa Mohamed Taher, Isa Mohamed Rose, Ramelah Mohamed,
author_sort	Asif Sukri,
title	Optimization of cryopreserved single cell suspension of gastric epithelial cells for successful DotScanTM antibody microarray
title_short	Optimization of cryopreserved single cell suspension of gastric epithelial cells for successful DotScanTM antibody microarray
title_full	Optimization of cryopreserved single cell suspension of gastric epithelial cells for successful DotScanTM antibody microarray
title_fullStr	Optimization of cryopreserved single cell suspension of gastric epithelial cells for successful DotScanTM antibody microarray
title_full_unstemmed	Optimization of cryopreserved single cell suspension of gastric epithelial cells for successful DotScanTM antibody microarray
title_sort	optimization of cryopreserved single cell suspension of gastric epithelial cells for successful dotscantm antibody microarray
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2019
url	http://journalarticle.ukm.my/13632/ http://journalarticle.ukm.my/13632/ http://journalarticle.ukm.my/13632/1/19%20Asif%20Sukri.pdf
first_indexed	2023-09-18T20:05:17Z
last_indexed	2023-09-18T20:05:17Z
_version_	1777407117290897408

Optimization of cryopreserved single cell suspension of gastric epithelial cells for successful DotScanTM antibody microarray

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